Objective: This study aimed to evaluate the vaccine hesitancy, anti-vaccination, and anxiety levels of medical secretaries during the pandemic. Methods: This cross-sectional study was conducted on 161 medical secretaries working at the time of the Study. Sociodemographic characteristics form, Coronavirus Anxiety Scale (CAS), Vaccine Hesitancy Scale (VHS) adapted to the pandemic, and Anti-vaccination Scale (AVS) were used in the questionnaire form used to collect the data of the study. Results: Median (IQR) CAS, VHS, and AVS scores of the participants were 2 (IQR=3), 32 (IQR=10), and 58 (IQR=16), respectively. 35.4 % of the participants were exposed to the COVID-19, and 87% were vaccinated against COVID-19. Participants' hesitations about the childhood and COVID-19 vaccines were 15.5 % and 49.1%, respectively. A significant relationship was found between the presence of Coronavirus anxiety and educational status (p=0.035), hesitancy against childhood vaccine (p=0.016), and working in COVID-19 Units (p=0.044). A statistically significant relationship was found between VHS scores and hesitancy against childhood vaccine (p=0.001), hesitancy against COVID-19 vaccine (p