Fatma ÇOLAKOĞLU, HASAN HÜSEYİN DÖNMEZ, Fatma KILIÇ

Determination of ANAE and AcP-ase Positivity in the Peripheral Blood of Pregnant Women with Hypothyroidism

Objective: Pregnancy is a stress factor for thyroid functions. The most commonly observed thyroid disorder is hypothyroidism. The immune system is affected both by pregnancy and hypothyroidism. This disorder causes a decrease in humoral and cell-mediated immune responses. This study was aimed at the comparison of alpha-naphthyl acetate esterase- and acid phosphatase-positive T lymphocyte distribution between healthy and hypothyroid pregnant women. Methods: Blood samples were taken from healthy and hypothyroid pregnant women between June-December 2016. The sampled subjects included 10 healthy non-pregnant women (HC), 10 healthy pregnant women (HP), and 30 hypothyroid pregnant women in different trimesters of pregnancy (HpPTRI, HpPTRII and HpPTRIII). From each of these groups, six smears were prepared and histologically stained for ANAE and AcP-ase activity and with May Grünwald-Giemsa. Results: The lowest ANAE- and AcP-ase-positive T lymphocyte percentages were detected in Group HP, and these percentages were higher in the hypothyroid pregnant groups, compared to Group HC (P

Hipotiroidli Gebe Kadınların Periferik Kanında ANAE ve AcP-az Pozitivitesinin Belirlenmesi

Amaç: Gebelik tiroid bezi fonksiyonları için bir stres faktörüdür. En sık görülen tiroid bozukluğu ise hipotiroidizmdir. Bağışıklık sistemi hem gebelikten hem de hipotiroidizmden etkilenmektedir. Bu bozukluk, humoral ve hücre aracılı immün cevaplarda azalmaya neden olmaktadır. Bu çalışma, sağlıklı gebeler ile hipotiroidli gebeler arasındaki alfa naftil asetat esteraz ve asit fosfataz pozitif T lenfosit dağılımlarını karşılaştırmayı amaçlamaktadır. Yöntemler: 2016 Haziran-Aralık ayları arasında sağlıklı ve hipotiroidi olan gebelerden kan örnekleri alındı. Örneklemede 10 sağlıklı gebe olmayan kadın (SK), 10 sağlıklı gebe (SG) ve gebeliğin farklı trimesterlerindeki 30 hipotiroidi gebe (HpGTRI, HpGTRII ve HpGTRIII) kadının kan örnekleri vardı. Bu gruplardaki kişilerin her birinden ANAE ve AcP-az enzim aktivitesi ile May Grünwald-Giemsa için altı adet kan frotisi hazırlanarak histolojik olarak boyandı. Bulgular: En düşük ANAE- ve AcP-az pozitif T lenfosit yüzdeleri sağlıklı gebe grubunda (Grup SG) belirlenirken; bu oranın hipotiroidli gebelerde (HpGTRI, HpGTRII ve HpGTRIII) sağlıklı gebe olmayanlardan (Grup SK) daha yüksek olduğu bulundu (P

PDF

___

1. Robertson SA, Petroff MG, Hunt JS. Knobil and Neill’s Physiology of Reproduction, 3rd edn. Philadelphia, USA: Elsevier, 2017: 1835-74.

2. Orgul G, Soyak B, Portakal O, et all. Total blood lymphocyte count alteration during and after pregnancy. GORM. 2017; 23: 11-3.

3. Stagnaro-Green A, Abalovich M, Alexander E, et all. Guideliness of the American Thyroid Association fort he diagnosis and management of the thyroid disease during pregnancy and postpartum. Thyroid. 2011; 21: 1081-125.

4. Abdelsamad DM, Ke R, Kutteh WH. Reference Module in Biomedical Sciences, 2nd edn. Philadelphia, USA: Elsevier, 2018: 1-5.

5. Karaca N, Akpak YK. Thyroid disorders and fertility. IJRMS. 2015; 3: 1299-304.

6. De Vito P, Incerpi S, Pederson JZ, et all. Thyroid hormones as modulators of immune activities at the cellular level. Thyroid. 2011; 21: 879-90.

7. Van Der Spek AH, Fliers E, Boelen A. Thyroid hormone metabolism in innate immune cells. J Endocrinol. 2017; 232: R67-R81.

8. Bayraktaroğlu AG, Şimşek Ö, Kürüm A, et all. Determination of alpha-naphthyl acetate esterase (ANAE) activity in peripheral blood leukocytes of pregnant, adult, and kitten Angora cats. Turk J Vet Anim Sci. 2015; 39: 57-61.

9. Dönmez HH, Eken E, Beşoluk K, et all. The histological characteristics and localisation of ACP-ase and ANAE positive lymphocytes in the eosophageal tonsil of the duck (Anas platyrhynchos). Avian Biol Res. 2012; 5: 11- 5.

10. Demir R, Yılmazer S, Öztürk M, et all. Histolojik Boyama Teknikleri Başvuru Kitabı, 1. baskı, Ankara, Palme Yayıncılık, 2007: 267-9.

11. Sahay Kumar R, Nagesh Sri V. Hypothyroidism in pregnancy. IJEM. 2012; 16: 364-70.

12. Warning JC, McCracker SA, Morris JM. A balancing act: mechanisms by which the fetus avoids rejection by the maternal immune system. Reprod. 2011; 141: 715-24.

13. Akbulut B, Sur E, Okur DN. Determination of the AgNOR parameters, MN frequency ANAE and ACP-ase positivity of PBL in pregnants. Selçuk Tıp Derg. 2015; 31: 344-50.

14. Sur E, Aydın İ, Öznurlu Y, ve ark. Merinos ırkı sağlıklı gebe koyunların perifer kan lenfositlerinde alfa naftil asetat esteraz ve asit fosfataz aktivitelerinin belirlenmesi. Kafkas Univ Vet Fak Derg. 2013; 19: 483- 8.

15. Pisek L, Travnicek J, Salat J, et all. Changes in white blood cells in sheep blood during selenium supplementation. Vet Med. 2008; 53: 255-9.

16. Lissauer DM, Eldershaw SA, Inman CF, et all. Progesterone promotes maternal-fetal tolerance by reducing human maternal T-cell polyfunctionality and inducing a spesific cytokine profile. Eur J Immunol. 2015; 45: 2858-72.

17.Bilandzic N, Simic B, Terzic S, et all. The influence of levamisole on cortisol concentration and peripheral blood in artificially stressed pigs. Vet Arc. 2010; 80: 477-89.

18. Vianna P, Bauer ME, Dornfeld D, et all. Distress conditions during pregnancy may lead to pre-eclampsia by increasing cortisol levels and altering lymphocyte sensivity to glucocorticoids. Med Hypotheses. 2011; 77: 188-91.

19. Stagnaro-Green A, Pearce E. Thyroid disorders in pregnancy. Nat Rev Endocrinol. 2012; 8: 650-8.

20. Korevaar TIM, Rijke de YB, Chaker L, et all. Stimulation of thyroid function by human chorionic gonadotropin during pregnancy: A risk factor for thyroid disease and a mechanism for known risk factors. Thyroid. 2017; 27: 440-50.

21. Rosol T, Walling BE. Immunopathology in Toxicology and Drug Development, 1st Edn. Switzerland: Springer Int Publishing AG, 2017: 649-94.

22. Gartner LP, Hiatt JL. Color Textbook of Histology, 3rd edn. Philadelphia, USA: Elsevier, 2007: 219-49.