Özlem ALTINDAĞ LEBLEBİCİ, Gülnur KAPLANOĞLU TAKE, Bahriye ARAL SİRAV, CEMİLE MERVE SEYMEN

Cep Telefonu Radyasyonunda Melatonin'in Testis Dokusunda Olası Koruyucu Etkisi

Amaç: Çalışmamızda cep telefonu radyasyonuna kronik etkin kalmanın testis dokusu üzerinde meydana getireceği değişimler ve melatonin kullanımının olası koruyucu etkisinin ışık mikroskobik düzeyde incelenmesi amaçlandı. Yöntemler: 24 adet Wistar albino cinsi erkek sıçan 4 gruba ayrılarak 90 günlük deney süresince; kontrol grubuna hiçbir uygulama yapılmazken, 2. gruba her gün 30 dk 2100 MHz radyasyon uygulaması, 3. gruba her gün derialtı melatonin uygulaması, 4. gruba ise radyasyon uygulamasından 40 dk önce derialtı melatonin ve sonrasında 30 dk radyasyon uygulaması yapıldı. Deney bitiminde yüksek doz anestezi altında feda edilen deneklerden testis dokuları alındı, ağırlıkları ölçülerek ışık mikroskobik izleme yöntemlerinden geçirildi. Örneklere yapısal değişimleri incelemek amacıyla Hematoksilen Eozin, bazal membran kalınlığının değerlendirilmesi amacıyla da PAS boyamaları yapıldı; germinal epitel kalınlığı ve seminifer tübül çapı ile bazal membran kalınlığı ölçülerek istatistiksel analizler yapıldı. Bulgular: Radyasyon uygulaması ile birlikte seminifer tübüllerde düzenli yapının bozulduğu ve seminifer epitel hücreleri arasında ödem oluştuğu izlendi. Radyasyon ve melatonin uygulanan grupta seminifer tübül yapısının radyasyon grubuna karşı oldukça korunduğu dikkati çekti. Tüm gruplarda testis ağırlığında istatistiksel olarak anlamlı bir farklılık saptanmazken, radyasyon uygulanan grupta bazal membranın daha kalın olduğu, germinal epitel kalınlığının azaldığı, seminifer tübül çapının ise arttığı istatistiksel olarak belirlendi. Sonuç: Sonuç olarak yüksek doz cep telefonu radyasyonunun testis histolojik yapısına, özellikle spermatogenezis ve spermiyogenezis düzeyinde etkili olduğu belirlenirken, melatoninin koruyucu özellik gösterdiği kanısına varıldı

Possible Protective Effect Of Melatonin On Testis In The Case Of Mobile Phone Radiation

Objective: Investigation the possible protective effect of melatonin on testis in the case of mobile phone radiation by using light microscopic examinations. Methods: 24 male Wistar albino rats were divided into 4 equal groups. Throughout in 90-day experiment, there was no application performed to control group. The second group was exposed to 2100 MHz radiation for 30 minutes. Subcutaneous melatonin was enjected to the third group. Subcutaneous melatonin enjection was applied 40 minutes before radiation and then radiation was exposed for 30 minutes to the fourth group. At the end of the experiment, testis tissues were taken from subjects which was sacrificed under high-dose anesthesia and also measured. Light microscobic monitoring methods were applied. In order to examine structural changes, slides were stained with Hematoxylin-Eosin and to evaluate the basal membrane, PAS staining were performed. Measurements of seminiferous tubules, germinal epithelium and basement membrane thickness were analyzed statistically. Results: Regular structure of seminiferous epithelium disrupted, edema was seen between seminiferous epithelium cells. In the radiation and melatonin group it was seen that, the structure of seminiferous tubule was highly protected. Testicular weight showed no differences between experimental groups statistically, but in radiation group the thickness of basement membrane and the diameter of the seminiferous tubule increased however, the thickness of germinal epithelium decreased according to statistical analyses. Conclusion: As a result, high frequency mobile phone radiation leads to some structural changes on testis especially in spermatogenesis and spermiogenesis levels. Also it was concluded that melatonin showed protective property against these changes

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