Alfa-lipoik asit, antioksidan etkisi ile sıçanlarda lipopolisakkarit kaynaklı karaciğer hasarını azaltır
Amaç: Potansiyel bir antioksidan olarak kabul edilen lipoik asidin (LA), karaciğer doku hasarında oksidatif stres parametreleri üzerindeki etkisi yeterince incelenmemiştir. Bu nedenle, sıçanlarda lipopolisakkaridle (LPS) indüklenen karaciğer hasarında LA'nın oksidan ve antioksidan parametrelerle ilişkisini incelemeyi amaçladık. Yöntemler: Birinci gruba (n=10), 20 mg/kg tek doz salin enjekte edildi (kontrol), ikinci gruba (n=10), tek doz 20 mg/kg LPS enjekte edildi (LPS) ve üçüncü gruba (n=10) 3 gün boyunca enjekte edilen 10 mg/kg LA sonrasında tek doz 20 mg/kg LPS enjekte edildi (LA+LPS). Glutatyon peroksidaz (GPX), katalaz (CAT) ve süperoksit dismutaz (SOD) aktiviteleri ve malondialdehid (MDA) düzeyleri spektrofotometrik yöntemle belirlendi. 3-Nitrotirosin (3-NT) seviyesi, yüksek performanslı sıvı kromatografisi ile ölçüldü. Bulgular: LA+LPS grubunda 3-NT ve MDA düzeylerinin LPS grubuna göre anlamlı derecede düşük olduğu bulundu. Ayrıca, LA+LPS'nin antioksidan enzim aktivite değerlerinin kontrol grubu değerlerine yaklaştığı bulundu. Sonuç: Bu bulgular, LA grubunda oksidan/antioksidan durumunun dengelendiğini göstermektedir. Bundan dolayı, LA takviyesinin, sıçanlarda LPS kaynaklı karaciğer hasarındaki oksidatif stresi önlemede faydalı olduğu öne sürülebilir
Alpha-lipoic acid alleviates lipopolysaccharide-induced liver damage in rats via antioxidant effect
Objective: The effect of lipoic acid (LA), which is considered to be a potential antioxidant, on oxidative stressparameters in liver tissue damage has not been sufficiently investigated. Therefore, we aimed to investigate therelationship of LA with oxidant and antioxidant parameters in lipopolysaccharide (LPS) induced liver damage in rats.Methods: First group (n=10) was injected a single dose of 20 mg/kg saline (control), second group (n=10) wasinjected a single dose of 20 mg/kg LPS (LPS) and third group (n=10) was injected a single dose of 20 mg/kg LPS at theend of third day of 10 mg/kg/day LA injection (LA+LPS). The glutathione peroxidase (GPX), catalase (CAT) andsuperoxide dismutase (SOD) activities and the levels of malondialdehyde (MDA) were determined byspectrophotometric method. 3-Nitrotyrosine (3-NT) level was measured by high performance liquid chromatography. Results: It was found that 3-NT and MDA levels were significantly lower in LA+LPS group than those of LPS group.Moreover, it was found that antioxidant enzyme activity values of LA+LPS approached to the values of control group.Conclusion: These findings indicate that the oxidant/antioxidant status is balanced in the LA group. Therefore, it maybe suggested that LA supplementation to be beneficial for preventing oxidative stress in LPS-induced liver damage inrats.
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- Erenler AK, Yapar D, Terzi Ö. Comparison of
Procalcitonin and C-reactive Protein in Differential
Diagnosis of Sepsis and Severe Sepsis in Emergency
Department. Dicle Med J. 2017; 44: 175-82.
- Ekinci A. Investigation of serum oxidative stress levels
in patients with nasal polyps. Dicle Med J. 2018; 45: 1-7
- Oluwole OG, Ologe O, Alabi A, Tunde YG, Umukoro S.
Anti-inflammatory effects and anti-oxidant capacity of
Myrathius arboreus (Cecropiaceae) in experimental
models. J Basic Clin Physiol Pharmacol. 2017; 28: 521-
9.
- Islam BU, Habib S, Ali SA, Moinuddin, Ali A. Role of
Peroxynitrite-Induced Activation of Poly(ADP-Ribose)
Polymerase (PARP) in Circulatory Shock and Related
Pathological Conditions. Cardiovasc. Toxicol. 2017; 17:
373-83.
- Ahsan H. 3-Nitrotyrosine: A biomarker of nitrogen free
radical species modified proteins in systemic
autoimmunogenic conditions. Hum Immunol. 2013; 74:
1392-9.
- Kurutas EB. The importance of antioxidants which play
the role in cellular response against
oxidative/nitrosative stress: current state. Nutr J. 2016;
15: 71.
- Huk-Kolega HB, Skibska P, Kleniewska A, Piechota Ł,
Michalski AG. “Rola kwasu liponowego w zdrowiu
ichorobie,” Polski Merkuriusz Lekarski. 2011; 31: 183–
5.
- Ozgun E, Ozgun GS, Usta U, et al. The effect of lipoic
acid in the prevention of myocardial infarction in
diabetic rats. Bratisl Lek Listy. 2018; 119: 664-9.
- Baranowska M, Bartoszek A. Antioxidant and
antimicrobial properties of bioactive phytochemicals
from cranberry. Postepy Hig Med Dosw. 2016; 70:
1460-8.
- Prauchner CA, Prestes Ade S, da Rocha JB. Effects of
diphenyl diselenide on oxidative stress induced by
sepsis in rats. Pathol Res Pract. 2011; 207: 554–8.
- Li X, Wang L, Han W, et al. Amentoflavone protects
against hydroxyl radical-induced DNA damage via
antioxidant mechanism. Turk J Biochem. 2014; 39: 30–
36.
- Güzel EE, Kaya N, Kocamüftüoğlu GO, et al.
Investigation of the Effects of Alpfa Lipoic Acid on
Changes in Maternal Rat Lung Caused by Tobacco
Smoke Exposure in Pregnancy Dicle Med J. 2018; 45:
297-307.
- Zalejska-Fiolka J, Wielkoszyński T, Kasperczyk S,
Kasperczyk A, Birkner E. Effects of oxidized cooking oil
and α-lipoic acid on liver antioxidants: enzyme
activities and lipid peroxidation in rats fed a high fat
diet. Biol Trace Elem Res. 2010; 138: 272-81.
- Lowry OH, Rosebrough NJ, Farr AL, Randall RJ.
Protein measurement with the Folin phenol reagent. J
Biol Chem. 1951; 193: 265-75.
- Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides
in animal tissues by thiobarbituric acid reaction. Anal
Biochem. 1979; 95: 351-8.
- Cimen B, Türközkan N, Seven I, Unlü A, Karasu C.
Impaired Na+, K+-ATPase activity as a mechanism of
reactive nitrogen species-induced cytotoxicity in guinea
pig liver exposed to lipopolysaccharides. Mol Cell
Biochem. 2004; 259: 53-7.
- Liu S, Adcock IM, Old RW, Barnes PJ, Evans TW.
Lipopolysaccharide treatment in vivo induces
widespread tissue expression of inducible nitric oxide
synthase mRNA. Biochem Biophys Res Commun. 1993;
196: 1208-13.
- Yasmineh WG, Kaur TP, Blazar BR, Theologides A.
Serum catalase as marker of graft-vs-host disease in
allogeneic bone marrow transplant recipients: pilot
study. Clin Chem. 1995; 41: 1574-80.
- Paglia DE, Valentine WN. Studies on the quantitative
and qualitative characterization of erythrocyte
glutathione peroxidase. J Lab Clin Med. 1967; 70: 158–
69.
- Sun Y, Oberley LW, Li Y. A simple method for clinical
assay of superoxide dismutase. Clin Chem. 1988; 34:
497–500.
- de S Moreira D, Figueiró PW, Siebert C, et al. Chronic
Mild Hyperhomocysteinemia Alters Inflammatory and
Oxidative/Nitrative Status and Causes Protein/DNA
Damage, as well as Ultrastructural Changes in Cerebral
Cortex: Is Acetylsalicylic Acid Neuroprotective?
Neurotox Res. 2018; 33: 580-92.
- Bharrhan S, Chopra K, Arora SK, Toor JS, Rishi P.
Down-regulation of NF-κB signalling by polyphenolic
compounds prevents endotoxin-induced liver injury in
a rat model. Innate Immun. 2012; 18: 70-9.
- Petronilho F, Florentino D, Danielski LG, et al. AlphaLipoic
Acid Attenuates Oxidative Damage in Organs
After Sepsis. Inflammation. 2016; 39: 357-65.
- El-Awady MS, Nader MA, Sharawy MH. The inhibition
of inducible nitric oxide synthase and oxidative stress
by agmatine attenuates vascular dysfunction in rat
acute endotoxemic model. Environ Toxicol Pharmacol.
2017; 55: 74-80.
- Petronilho F, Florentino D, Danielski LG, et al. AlphaLipoic
Acid Attenuates Oxidative Damage in Organs
After Sepsis. Inflammation. 2016; 39: 357-65.
- Zhang HX, Liu SJ, Tang XL, et al. H2S Attenuates LPSInduced
Acute Lung Injury by Reducing
Oxidative/Nitrative Stress and Inflammation. Cell
Physiol Biochem. 2016; 40: 1603-12.
- Paradies G, Paradies V, Ruggiero FM, Petrosillo G.
Protective role of melatonin in mitochondrial
dysfunction and related disorders. Arch Toxicol. 2015;
89: 923-39.
- Choi YJ, Kim HS, Lee J, et al. Down-regulation of
oxidative stress and COX-2 and iNOS expressions by
dimethyl lithospermate in aged rat kidney. Arch Pharm
Res. 2014; 37: 1032-8.
- Sadi G, Kartal Dİ, Güray T. Regulation of Glutathione
S-Transferase Mu with type 1. Turk J Biochem. 2013;
38; 92–100.
- Safa J, Ardalan MR, Rezazadehsaatlou M, et al. Effects
of alpha lipoic acid supplementation on serum levels of
IL-8 and TNF-α in patient with ESRD undergoing
hemodialysis. Int Urol Nephrol. 2014; 46: 1633-8.