Suleyman Emre MEŞELİ, Gonca ERKÖSE GENÇ, Gizem ÇOLAKOĞLU, Suleyman PELİT, Esra KOÇ, Birsay GÜMRÜ TARÇIN

THE EVALUATION OF THE COLONIZATION AND BIOFILM FORMATION CAPACITY OF THE CANDIDA SPECIES ISOLATED FROM DENTURE WEARER PATIENTS

Abstract Aim: CAim: Candida species, although being a member of the oral flora, may exhibit pathological features under certain conditions. The aim of this study was to determine the distribution and biofilm production of Candida species that were isolated from denture related stomatitis (DRS) patients and to compare with healthy subjects. andida species, although being a member of the oral flora, may exhibit pathological features under certain conditions. The aim of this study was to determine the distribution and biofilm production of Candida species that were isolated from denture related stomatitis (DRS) patients and to compare with healthy subjects. Materials and Methods: The 56 non-smoker, systemically healthy, maxillary removable denture wearer subjects included in this study and diagnosed as DRS(N=27) and healthy(N=29). Samples from the palatal mucosal surface of patients were collected by sterile swabs during 20 seconds. Cultivation and selective isolation of Candida species were performed on CHROMagar (CHROMagar® Candida, CHROMagar, Paris, France) at 37ºC for 2 days. Isolated Candida species were identified with API ID 32 C (bioMérieux®, France). Biofilm formation by Candida species were determined by a visual tube method. Results: The prevalence of the yeasts in the healthy group was found to be 37.94%, while in the DRS group it was 100% (p<0.001). The isolation rate of C. albicans in patients with DRS significantly higher (p<0.001). Biofilm formation was observed in a total of 37 oral yeast isolates, 9 isolates from healthy denture wearers and 28 isolates from DRS patients. The number of C. albicans and C. glabrata isolates showing biofilm formation ability in the DRS group was significantly higher than the healthy group (p<0.05). Conclusions: Within the limitations of this study, our results suggest that Candida species play a major role on the development of DRS. While C. albicans was the most isolated species in DRS, C. glabrata was as important as C. albicans on the pathogenesis of DRS. keywords: Biofilms, Candida, denture stomatitis, mycology, oral pathology HAREKETLİ PROTEZ KULLANAN HASTALARDA KANDİDA TÜRLERİNİN KOLONİZASYONUNUN VE BİYOFİLM OLUŞTURMA KAPASİTELERİNİN DEĞERLENDİRİLMESİ Öz Amaç: Candida türleri, oral floranın doğal üyesi olmakla birlikte, belirli koşullar altında patolojik özellikler sergileyebilir. Bu çalışmanın amacı, protez stomatiti (PS) olan hastalardan ve protez kullanan sağlıklı bireylerden elde edilen Candida türlerinin dağılımını ve biyofilm üretime kapasitelerini karşılaştırmaktırGereç ve Yöntem: Sigara içmeyen, sistemik olarak sağlıklı, maksiller tam damak protez kullanan 56 kişi bu çalışmaya dahil edildi. Katılımcılar PS (N = 27) ve sağlıklı (N = 29) olmak üzere gruplandırıldı. Hastaların palatal mukoza yüzeyinde 20 saniye boyunca steril swablar gezdirilerek sürüntü örnekleri elde edildi. Candida türlerinin kültivasyon ve seçici izolasyonu 2 gün boyunca 37 ° C'de CHROMagar (CHROMagar® Candida, CHROMagar, Paris, Fransa) besiyerinde gerçekleştirildi. İzole edilen Candida türlerinin identifikasyonu API ID 32 C (bioMérieux®, Fransa) ile gerçekleştirildi. Candida türlerinin biyofilm oluşturma potansiyelleri görsel tüp yöntemi ile saptandıBulgular: Sağlıklı gruptaki mayaların prevalansı % 37.94 iken, PS grubunda % 100'dü (p <0.001). PS'li hastalarda C. albicans'ın izolasyon oranı anlamlı derecede daha yüksekti (p <0.001). Sağlıklı katılımcılardaki 9 PS katılımcılardaki 28 izolatta biyofilm oluşumu gözlendi. PS grubunda biyofilm oluşumunu gösteren C. albicans ve C. glabrata izolatlarının sayısı sağlıklı gruba göre anlamlı olarak yüksekti (p <0.05). Sonuç: Bu çalışmanın sınırları dahilinde, sonuçlarımız Candida türlerinin PS'nin gelişiminde önemli bir rol oynadığını göstermektedir. C. albicans, PS'de en sık izole edilen tür olsa da C. glabrata da PS'nin patogenezinde C. albicans kadar önemlidir. Anahtar Kelimeler: Biyofilm, Kandida, protez stomatitisi, mikoloji, oral patoloji

Anahtar Kelimeler:

Biofilms, Candida, denture stomatitis, mycology

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1. Gumru B, Tanju K, Uygun-Can B, Ozbayrak S. Distribution and phospholipase activity of Candida species in different denture stomatitis types. Mycopathol 2006;162:389-94.
2. Uludamar A, Ozyesil AG, Ozkan Kulak Y. Clinical and microbiological efficacy of three different treatment methods in the management of denture stomatitis. Gerodontology 2011;28:104-10.
3. Yarborough A, Cooper L, Dugum I, Mendonça G, McGraw K, Stoner L. Evidence regarding the treatment of denture stomatitis. J Prosthodont 2016;25:288-301.
4. Guggenheimer J, Moore PA, Rossie K, Myers D, Mongelluzzo MB, Block HM, Weyant R, Orchard T. Insulin-dependent diabetes mellitus and oral soft tissue pathologies: II. Prevalance and characteristics of Candida and Candidal lesions. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2000;89:570-6.
5. Dorocka-Bobkowska B, Zozulinska-Ziolkiewicz D, Wierusz-Wysocka B, Hedzelek W, Szumala-Kakol A, Budzt-Jörgensen E. Candida-associated denture stomatitis in type 2 diabetes mellitus. Diabetes Res Clin Pract 2010;90:81-6.
6. Ercalik-Yalcinkaya S, Ozcan M. Association between oral mucosal lesions and hygiene habits in a population of removable prosthesis wearers. J Prosthodont 2015;24:271-8.
7. Nevalainen MJ, Narhi TO, Ainamo A. Oral mucosal lesions and oral hygiene habits in the home-living elderly. J Oral Rehabil 1997;24:332-7.
8. Marcos-Arias C, Vicente JL, Sahand IH, Eguia A, De-Juan A, Madariaga L, Aguirre JM, Eraso E, Quindos G. Isolation of Candida dubliniensis in denture stomatitis. Arch Oral Biol 2009;54:127-31.
9. Barbeau J, Seguin J, Goulet JP, de Koninck L, Avon SL, Lalonde B, Rompre P, Deslauriers N. Reassessing the presence of Candida albicans in denture-related stomatitis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2003;95:51-9.
10. Yigit N, Aktas E, Dagistan S, Ayyildiz A. Investigating biofilm production, coagulase and hemolytic activity in Candida species isolated from denture stomatitis patients. Eurasian J Med 2011;43:27-32.
11. Finkel JS, Mitchell AP. Genetic control of Candida albicans biofilm development. Nat Rev Microbiol 2011;9:109-18.
12. Mohandas V, Ballal M. Distribution of Candida species in different clinical samples and their virulence. biofilm formation, proteinase and phospholipase production: a study on hospitalized patients in southern India. J Glob Infect Dis 2011;3:4-8.
13. Nett JE. Future directions for anti-biofilm therapeutics targeting Candida. Expert Rev Anti Infect Ther 2014;12:375-82.
14. Sardi JC, Scorzoni L, Bernardi T, Fusco-Almeida AM, Mendes Gianninni MJ. Candida species: current epidemiology, pathogenicity, biofilm formation, natural antifungal products and new therapeutic options. J Med Microbiol 2013;62:10-24.
15. Nweze EI, Ghannoum A, Chandra J, Ghannoum MA, Mukherjee PK. Development of a 96-well catheter-based microdilution method to test antifungal susceptibility of Candida biofilms. J Antimicrob Chemother 2012;67:149-63.
16. Nikawa H, Egusa H, Makihira S, Okamoto T, Kurihara H, Shiba H, Amano H, Murayama T, Yatani H, Hamada T. An in vitro evaluation of the adhesion of Candida species to oral and lung tissue cells. Mycoses 2006;49:14-7.
17. Bilhan H, Sulun T, Erkose G, Kurt H, Erturan Z, Kutay O, Bilgin T. The role of Candida albicans hyphae and Lactobacillus in denture-related stomatitis. Clin Oral Invest 2009;13:363-8.
18. Silness J, Loe H. Periodontal disease in pregnancy. ii. correlation between oral hygiene and periodontal condition. Acta Odonto Scand 1964;22:121-35.
19. Gokce G, Cerikcioglu N, Yagci A. Acid proteinase, phospholipase, and biofilm production of Candida species isolated from blood cultures. Mycopathologia 2007;164:265-9.
20. Uygun-Can B, Kadir T, Gumru B. Effect of oral antiseptic agents on phospholipase and proteinase enzymes of Candida albicans. Arch Oral Biol 2016;62:20-7.
21. Kadir T, Pisiriciler R, Akyuz S, Yarat A, Emekli N, Ipbuker A. Mycological dna cytological examination of oral candida carriage in diabetic patients and non-diabetic control subjects: through analysis of local aetiologic and systemic factors. J Oral Rehabil 2002;29:452-7.
22. Samaranayake LP, MacFarlane TW. Factors affecting the in vitro adherence of the fungal oral pathogen Candida albicans to epithelial cells of human origin. Arch Oral Biol 1982;27:869-73.
23. Ghannoum MA. Potential role of phospholipases in virulence and fungal pathogenesis. Clin Microbiol Rev 2000;13:122-43.
24. Dar-Odeh NS, Sherabi AA. Oral candidosis in patients with removable dentures. Mycoses 2003;46:187-91.
25. Coco Bj, Bagg J, Cross LJ, Jose A, Cross J, Ramage G. Mixed Candida albicans and Candida glabrata populations associated with the pathogenesis of denture stomatitis. Oral Microbiol Immunol 2008;23:377-83.
26. Gaucha LMR, Pedrosa SS, Silveira-Gomes F, Esteves RA, Marques-da-Silva SH. Isolation of Candida spp. from denture-related stomatitis in Pará, Brazil. Braz J Microbiol 2018;49:148-51.
27. Dorocka-Bobkowska B, Konopka K, Duzgunes N. Influence of antifungal polyenes on the adhesion of Candida albicans and Candida glabrata to human epithelial cells in vitro. Arch Oral Biol 2003;48:805-14.
28. Darwazeh AM, Hammad MM, Al-Jamaei AA. The relationship between oral hygiene and oral olonization with Candida species in healthy adult subjects. Int J Dent Hyg 2010;8:128-33.
29. Moalic E, Gestalin A, Quinio D, Gest PE, Zerilli A, Le Flohic AM. The extent of oral fungal flora in 353 students and possible relationships between dental caries. Caries Res 2001;35:49-55.
30. Wang H, Wang Y, Chen J, Zhan Z, Li Y, Xu J. Oral yeast flora and its sequence diversity among a large cohort of medical students in Hainan, China. Mycopathologia 2007;164:65-72.