Merve ERGÜL, Ahmet ALTUN, Şenay ÇETİNUS, Tijen KAYA, Eren ÇETİNUS, Tuna TEMİZ

Thyme essential oil inhibits proliferation of DLD-1 colorectal cancer cells through antioxidant effect

Amaç. Kekik uçucu yağı gibi biyolojik aktif ekstraktların çeşitli kanser hücreleri üzerindekietkilerini ve etki mekanizmalarını araştırma konusu oldukça önemli bir konudur. Bu çalışmadakekik uçucu yağının insan DLD-1 kolorektal kanser hücrelerinin büyümesini inhibe edip etmediği,eğer ediyorsa bu inhibisyona antioksidan etkisinin katkısını belirlemek amaçlanmıştır. Yöntem.İnsan DLD-1 kolorektal kanser hücrelerine kekik uçucu yağı uygulayarak, uçucu yağın hücreproliferasyonu üzerindeki etkilerini gerçek zamanlı hücre analiz sistemi (Xcelligence Sistemi) iledeğerlendirdik. Ayrıca kekik uçucu yağının antikanser etkinlik gösterdiği konsantrasyonlarınnormal hücrelerde sitotoksik olup olmadığını belirlemek amacıyla L929 fibroblast hücrelerine deaynı konsantrasyonları uyguladık. Daha sonra kekik uçucu yağının antioksidan etkinliğini DPPHyöntemini kullanarak değerlendirdik. Bulgular. Kekik uçucu yağı uygulanan DLD-1 hücreleri,konsantrasyona bağımlı olarak hücre indeksinde azalan değerler gösterdi. IC50 değeri 0,347mg/mL olarak bulundu. Diğer taraftan kekik uçucu yağı L929 fibroblast hücreleri üzerineuygulandığında sitotoksik etkisi gözlenmedi. Bu deney için IC50 değeri 22 mg/mL olarak bulundu.Bunun yanında kekik uçucu yağı DPPH yönteminde güçlü antioksidan etkinlik gösterdi. Sonuç.Kekik uçucu yağının antiproliferatif ve antioksidan etkileri dikkate alındığında, kolorektal kansertedavisinde alternatif bir seçenek olabileceği sonucuna varılmaktadır.

Kekik uçucu yağı antioksidan etki aracılığı ile DLD-1 kolorektal kanser hücrelerinin prolif erasyonunu inhibe ediyor

Aim. It is a very hot topic to investigate the effects and the mechanisms of action of biologicalactive extracts such as thyme essential oil on various cancer cells. This paper examines the abilityof thyme essential oil to inhibit the growth of DLD-1 colorectal cancer cells and if it is the case,determine the contribution of antioxidant effect to this ability. Methods. To do this, we treatedhuman DLD-1 colorectal cancer cells with thyme essential oil and assessed its effects on cellproliferation by using real time cell analyzing system (Xcelligence System). Furthermore, weapplied the same concentrations to the L929 fibroblast cells in order to determine if thymeessential oil has cytotoxicity on normal cells in the same concentrations it showed anticancereffects. Then, antioxidant ability of thyme essential oil was evaluated by using DPPH assay.Results. Thyme essential oil treated DLD-1 cells exhibited decreasing cell index values in aconcentration-dependent manner. An inhibitor concentration 50 (IC50) value of 0.347 mg/mL wasachieved. On the other hand, when thyme essential oil was tested on L929 fibroblast cells, it wasobserved that thyme essential oil did not show cytotoxic effect on L929 fibroblast cells. IC50 valuewas 22 mg/mL for this experiment. Besides, thyme essential oil showed a strong antioxidant effecton DPPH assay. Conclusion. When the antiproliferative and the antioxidant effects of thymeessential oil are considered, it can be concluded that thyme essential oil may be an alternativeoption in the treatment of colorectal cancer.

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