Ömer POYRAZ, Fahrettin GÖZE, Metin ÇALIŞIR, Derya Özdemir DOĞAN, Vildan BOSTANCI, Aysun AKPINAR

Ratlarda deneysel periodontitis modellerinde sistemik ve lokal interlökin 1BETA(IL-1?) ve IL-10 seviyelerine ozonun etkisi

Amaç: Bu çalışmanın amacı deneysel periodontitisli ratlarda ozonun uygulanmasıyla histopatolojik ve morfometrik değişiklikleri analiz etmektir. Yöntem: 24 rat rastgele olarak üç gruba ayrıldı. Bu gruplar: ligatürsüz tedavi grubu (NL) (n=8), sadece ligatür uygulanan tedavi grubu (LO) (n=8) ve ligatür+ozon uygulanan tedavi grubu olarak belirlendi (LOZ) (n=8). Deneysel periodontitis oluşturmak için ratların sağ mandibular 1. molarlarının gingival marjinlerine 4/0 ipek sutur yerleştirildi. 14 gün sonra ratlar sakrifiye edildi. Her gruptaki ratların alveolar kemik seviyelerindeki değişiklikler klinik olarak ölçüldü, ve her gruptaki ratların osteoklast morfolojileri, osteoklast ve osteoblast aktiviteleri, enflamatuar hücre infiltrasyonlarını (ICI) belirlemek için dokuları histopatolojik olarak incelendi. Serum ve sitokin seviyeleri ratlara özel IL-1? ve IL-10 Eliza kitleri kullanılarak ölçüldü. Bulgular: Mandibular diş çevresindeki alveolar kemik kaybı NL ve LOZ grupları ile karşılaştırıldığında LO grubunda önemli oranda yüksek bulundu. (p

THE effects of ozone on the local and systemic interleukin 1? (IL-1?) and IL-10 levels experimental periodontitis model in rats

Objective: The purpose of this study was to analyze the morphometric and histopathologic changes associated with experimental periodontitis in rats in response to the administration of ozone. Method: Twenty-four rats were randomly divided into three experimental groups: a nonligated (NL) treatment group (n = 8), a ligature-only (LO) treatment group (n = 8), and a ligature plus ozone (60 second/each tooth a day) (LOZ) treatment group. In order to induce experimental periodontitis, a 4/0 silk suture was placed at the gingival margin of the right-mandibular first molars of the rats. The study duration was 14 days, and then the animals were sacrified. Changes in the alveolar bone levels of rats in each group were measured clinically, and the tissues of the rats in each group were examined histopathologically to determine inflammatory cell infiltration (ICI), osteoblast and osteoclast activities, and osteoclast morphology. Serum and gingival cytokine levels were measured by using the rat-specific IL-1? and IL-10 ELISA kits. Results: Alveolar bone loss around the mandibular molar tooth was significantly higher in the LO group compared with NL and LOZ groups (p

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