Deniz TORUN, Erhan PARILTAY, Şefik GÜRAN, Salih KOZAN, Ferit AVCU, Ali Uğur URAL, Zehra Dilşad ÇOBAN

Low Zip 4 gene expression levels in RPMI - 8226 and ARH - 77 cell lines support the possible role of zip 4 transporter protein in plasma cell tumorogenesis

Amaç. Mltipl myelom ve plazma hücreli lösemi, plazma hücresinden köken alan kanserlerdir.Hastalığın ileri evresinde multipl myelom nadiren plazma hücreli lösemi formuna döner. Kimyasalbir element olarak çinko farklı büyüme faktörleri üzerinden memeli hücrelerinde çoğalma vefarklılaşmayı düzenler. Taşıyıcı bir protein olarak Zip 4 ise hücrede çinko metabolizmasında aktifrol alır. Pankreas ve karaciğer kanserlerinde artmış Zip 4 gen ekspresyonu bulunurken, prostatkanserinde düşük Zip 4 gen ekspresyonu tanımlanmaktadır. Yöntemler. Bu çalışmada, sırası ilemultipl myelom ve plazma hücreli lösemilere örnek olabilecek RPMI -8226 ve ARH -77 hücrehatlarında Zip 4 gen ekspresyon düzeyleri bakılmıştır. Bulgular. Her iki hücre hattında Zip 4 genekspresyon düzeyi normale göre daha düşüktür (RPMI -8226 hücre hattında 0,000157 ve ARH - 77 hücre hattında 0,000227). Elde edilen veriler istatistiksel olarak anlamlıdır (P < 0,05). Her ikihücre hattında Zip 4 gen ekspresyon düzeyleri birbirine çok yakın olarak bulunmuştur. Aralarındaistatistiksel olarak anlamlı bir fark da bulunmamıştır (P = 0,547). Sonuç. Sonuçlar plazma hücrediskrazilerinde Zip 4 gen ekspresyonunun olası rolünü destekler niteliktedir. Her iki hücre hattındabirbirine yakın bulunan Zip 4 gen ekpresyon düzeyi Zip 4'ün multipl myelomanın plazma hücrelilösemiye dönüşmesinde rolü olmadığını ortaya koymaktadır.

RPMI-8226 ve ARH-77 hücre hatlarında düşük Zip 4 gen eksp resyon düzeyi bulgusu p lazma hücrelerinden köken alan tümörlerin gelişiminde Zip 4 taşıyıcı p roteininin olası rolünü desteklemektedir

Aim. Multiple myeloma and plasma cell leukemia are cancers of plasma cells. Multiple myelomararely transforms to plasma cell leukemia during the progression period. Zinc as a chemicalelement modulates proliferation and differentiation of cells by affecting several growth factors. Zip4 modifies zinc metabolism in a cell as a transporter protein. While high Zip 4 gene expressionwas found in pancreas and hepatocellular carcinoma, low Zip 4 gene expression was observed inprostate carcinoma. Methods. Here, Zip 4 expression levels were studied in RPMI -8226 andARH -77 cell lines as examples of multiple myeloma and plasma cell leukemia, respectively.Results. We found lower Zip 4 gene expression levels in both cell lines than that of the normalcontrol (0,000157 in RPMI -8226, 0,000227 in ARH -77 cell lines and 1 in normal control) Thefindings were statistically significant (P < 0.05). The expression levels of Zip 4 gene in both celllines were approximately similar. No statistical significance was found between the expressionlevels of Zip 4 in both cell lines (P = 0.547). Conclusion. The results of this study support thepossible role of Zip 4 gene expression in plasma cell dyscrasias. The similar result of Zip 4 geneexpression level in both cell lines may show that Zip 4 has no role in the transformation ofmultiple myeloma to plasma cell leukemia.

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