Yavuz SİLİĞ, Serap BÖLÜKBAŞI ŞAHİN, Hüseyin ANIL, Safiye ÖZTÜRK EMİRDAĞ

Effects of egonol that isolated from seeds of Styrax officinalis (L.) on lipid peroxidation and antioxidant defense system in human erythrocytes

Bu çalışmada; Styrax officinalis L. tohumlarından izole edilen egonolün, insan eritrositleri antioksidan savunma sistemi üzerine etkisinin araştırılması amaçlanmıştır. Yöntem: Egonolün deney ortamındaki son derişimleri 0.2, 0.4, 0.6, 0.8 ve 1 mg/ml olacak şekilde eritrositler üzerine eklendi ve eritrosit peletler 15 dakika süreyle inkübe edildi. İnsan eritrosit hemolizatlarında; glutatyon-s-transferaz (GST), katalaz (KAT), süperoksit dismutaz (SOD) ve glutatyon peroksidaz (GPx) enzim aktiviteleri ile malondialdehit (MDA) düzeyleri belirlendi. Bulgular: Egonol uygulanan insan kan eritrosit grupları, kontrol grubuyla karşılaştırıldığında, SOD ve GST enzim aktivitelerinde doz bağımlı olarak belirgin artışlar gözlendi (p

İnsan eritrositlerinde lipid peroksidasyonu ve antioksidan defans üzerine Styrax officinalis (L.) tohumlarından izole edilen egonol'ün etkileri

The aim of the present study was to investigate the effects on antioxidant defense system in human erythrocytes exposed to egonol that isolated from seeds of Styrax officinalis L. Method: A solution of Egonol was added so that final concentrations of Egonol were 0.2, 0.4, 0.6, 0.8, and 1 mg/ml and erythrocyte pellets were incubated for 15 minutes. Malondialdehyde (MDA) level, activities of glutathione peroxidase (GPx), catalase (CAT), superoxide dismutase (SOD), glutathione-S-transferase (GST) glutathione were determined in hemolysis of human erythrocytes. Results: Activities of SOD and GST were markedly increased in erythrocyte of human blood compared to the control group (p

PDF

___

Davis P H. Flora of Turkey and the East Aegean Islands[M]. Vol. 4. University of Edinburgh Press, Edinburgh: 1972.
Vardar V, Oflas S. Preliminary studies on the Styrax oil[J]. S Qual Plant Mater Veg 1973; 22: 145-8.
Takanashi M, Takizawa Y. New egonol glycoside having D-xylose from immature seeds of styrax obassia Sieb. et Zucc. J Oleo Sci ; 51: 423-6. Pauletti PM, Araşjo AR, Young MC, et al. nor-Lignans from the leaves of Styrax ferruyineus (Styracaceae) with antibacterial and Phytochemistry 2000; 55:597-601
Min BS, Na MK, Oh SR, Ahn et al. New furofuran and butyrolactone lignans with antioxidant activity from the stem bark of Styrax japonica [J]. J Nat Prod 2004; :1980-4.
Hirano T, Gotoh M, Oka K. Natural flavonoids and lignans are potent cytostatic agents against human leukemic HL-60 cells [J]. Life Sci 1994; 55: 1061-9.
Teles HL, Hemerly JP, Paulettit PM, et al. Cytotoxic lignans from the stems of Styrax camporum (Styracaceae) [J]. Nat Prod Res ; 19: 319-23. Oztürk SE, Akgül Y, Anil H. Synthesis and antibacterial activity of egonol derivatives [J]. Bioorg Med Chem 2008; 16: 4431-7
Emirdağ-Öztürk S, Karayildirim T, Anil H. Synthesis of egonol derivatives and their antimicrobial activities [J]. Bioorg Med Chem ; 19: 1179-88
Reiter C, Capcı Karagöz A, Fröhlich T, et al. Synthesis and study of cytotoxic activity of 1,2,4- trioxane- hybrid Plasmodium multidrug-resistant leukemia cells [J]. Eur J Med Chem 2014; 75: 403-12. against falciparum and human
Miller HE, Rigelhof F, Marquart L, et al. Whole grain products and antioxidants [J]. Cereal Food world 2000; 45: 59-63.
Joshipura KJ, Hu FB, Manson JE, et al. The effect of fruit and vegetables intake on risk for coronary Annuals of Internal Medicines ; 134: 1106-14. J]. Arouma OI. Methodological consideration for characterizing potential antioxidant actions of bioactive compounds in plant foods [J]. Mutation Research ; 9: 523-4.
Sadrazadeh SMH, Graf E, Panter SS, et al. Hemoglobin, a biological Fenton reagent [J]. Journal of Biological Chemistry 1984; 259: -6.
McCord JM. Oxygen-derived free radicals in postischemic tissue injury [J]. N Engl J Med. Jan ; 312: 159-63. Halliwell B and Gutteridge JMC. Free radicals in biology and medicine Press.Oxford. 1989. Clarendon
Dodge JT, Mitchell C, Hanahan J. The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes [J]. Archives Biophysics 1963; 100: 119-30. and Drabkin DL. The crystallographic and optical properties of the hemoglobin of man in comparison with those of other species [J]. The Journal of Biological Chemistry ; 164: 703-23.
Warholm M, Guthenberg C, Bohr CV Transferases from human liver [J]. Method Enzymol 1985; 113: 499- McCord Glutathione-S- JM, Fridovich I. An for enzymic erythrocuprein (hemocuprein) [J]. J Biol Chem 1969; 244: 6049-55. Worthington Enzyme Worthington
Corporation Freehold, New Jersey, pp.77-80. 1993 Worthington Catalase, Biochemical
Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase [J]. Lab Clin Med 1967; 70: 158-69.
Yagi K. Assay for blood plasma or serum Enzymology 1984; 105: 328-31. in
Min BS, Oh SR, Ahn KS, et al. Anti-complement activity of erythrocytes J]. Bukowska B, Damage to erythrocytes caused by 2,3,7,8- tetrachlorodibenzo-p-dioxin vitro) [J]. Cellular Molecular Biology Letters 2004; 9: 261-70.
Bukowska B, Kowalska S, Phenol and catechol induce prehemolytic and hemolytic changes in human erythrocytes [J]. Toxicology Letters 2004; 152: 73-84.
Chasseaud LF. The role of glutathione and glutathione S- transferases in the metabolism of chemical carcinogens and other electrophilic agents [J]. Adv Cancer Res 1979; 29:175-274.
Cervello I, Lafuente A, Giralt M, et al. Enhanced glutathione S- transferase (GST) activity in pregnant benzo(a)pyrene [J]. Placenta ; 13: 273-80. with Mosialou E, Morgenstern R. Activity of rat liver microsomal glutathione transferase toward products of lipid peroxidation and studies of the effect of inhibitors on protection peroxidation [J]. Arch Biochem Biophys 1989; 275: 289-94.
Hayes JD, Pulford DJ. The glutathione supergene family: regulation of GST and the contribution of the isoenzymes chemoprotection resistance [J]. Crit Rev Biochem Mol 1995; 3: 445-600. cancer drug and Fiander H, Schneider H.
Compounds that induce isoforms of glutathione S-transferase with properties of a critical enzyme in defense against oxidative stress J]. Biochem Bioph Res Co 1999; : 591-95.
Gutteridge JMC, Halliwell B. Antioxidants: ormedia hype? Antioxidants in nutrition, health and disease, Oxford University Press. 1992; 40- Halliwell elixirs of life B. Free radicals, antioxidants, and human disease: curiosity, cause, or consequence J]? Lancet 1994; 344: 721-4.
Combs GF, Clark LC. Can dietary selenium modify cancer risk[J]? Nutrition Reviews, 1985; 43: 325- Freeman BA, Crapo JD. Hyperoxia radical production in rat lungs and lung mitochondria[J]. J Biol Chem ; 256: 10986-92. oxygen
Baskaran S, Lakshimi S, Prasad PR. Effect of cigarette smoke on lipid peroxidation and antioxidant enzymes in albino rat [J]. Indian J Exp Biol 1999; 37: 1196-200.