Anticancer and antiangiogenic effects of methanol extracts of Lonicera caprifolium L. on C6 rat glioma cells

Amaç: Gliomolar yüksek morbitite ve mortalite oranı olan beyin tümörleridir. Gliomaların tedavisi için, mevcut klinik tedaviye tamamlayıcı olabilecek yeni ve güçlü tedavi çözümleri geliştirmek önemlidir. Lonicera caprifolium L.'nin (L. caprifolium) bitkisel geleneksel tıpta çeşitli kullanımları vardır. Bu çalışma, Lonicera caprifolium L. (Bahçe Hanımelisi) ekstraktının fenolik asit düzeylerini ve DNA hasarı koruma potansiyelini belirlemek ve C6 sıçan glioma hücre hatları ve normal L929 fare fibroblast hücre hatları üzerindeki toksisitesinin inceleyerek Lonicera caprifolium ekstraktının anti-tümör etkisini araştırmak için gerçekleştirilmiştir. Bu çalışmada ayrıca ekstraktın antianjiyogenik potansiyelinin araştırılması amaçlanmıştır. Yöntem: Ekstraktların fenolik asit içeriği, HPLC analizi ile tespit edildi. DNA hasarı koruma potansiyeli pBR322 plazmid DNA üzerinde değerlendirildi. Ekstraktın kanser hücrelerinin proliferasyonu üzerindeki etkisi XTT testi ile değerlendirildi. Anti-anjiyogenik etki koryoallonterik membran modeli ile tespit edildi. Bulgular: Ekstrakt vanillik asitten (273,003 µg/g), zengin bulundu; klorogenik asit miktarı hemen hemen göz ardı edilebilir seviyede (0,028 µg/g) tespit edildi. 0.005-0.05 mg/ml aralığındaki ekstrakt, tüm DNA bantlarını UV ve H2O2' nin tehlikeli etkisine karşı korudu. Kontrol grubu ile karşılaştırıldığında ekstraktın varlığı önemli ölçüde C6 hücre çoğalmasını azalttı (p

C6 sıçan glioma hücreleri üzerinde Lonicera caprifolium L. metanol ekstraktlarının antikanser ve antianjiyogenik etkileri

Objective: Gliomas are brain tumors with high morbidity and mortality. For the treatment of gliomas, it is important to develop new and powerful treatments that could complement existing clinical treatment. Lonicera caprifolium L. (L. caprifolium) has various uses in herbal traditional medicine. This study was conducted to determine the phenolic acid levels and DNA damage protection potential of L. caprifolium extract, and to explore the antitumor effect of the extract by investigating its toxicity on C6 rat glioma cell lines and normal L929 mouse fibroblast cell lines. We also aimed to investigate the antiangiogenic potential of the extract. Method: Phenolic acid content was determined by HPLC analysis. DNA damage protection potential was evaluated on pBR322 plasmid DNA.The effect of extracts on the proliferation of cancer cells was evaluated by XTT assay. Antiangiogenic effect was determined with Chorioallantoic membrane model. Results: The extract was found rich in vanillic acid (273.003 µg/g); while the amount of chlorogenic acid was almost at negligible level (0.028 µg/g). 0.005-0.05 mg / ml extract protected against the hazardous effects of UV and H2O2 in all DNA bands. The presence of the extract significantly reduced C6 cell proliferation compared to control (p

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