Mastitis Patojenlerine Karşı Ocimum basilicum L.’ un Antibakteriyel ve Antioksidan Aktiviteleri

En yaygın bakteriyel ajanlar meme bezlerinde iltihaplanmaya (mastitis) neden olmakta, ki bu hastalık ineklerde düşük verime neden olur. Bu bakteriler Staphylococcus aureus ve koagülaz-negatif Staphylococci (KNS)’dir. Bu çalışmanın amacı, Ocimum basilicum L.’nin çeşitli özütlerinin antibakteriyel ve antioksidan potansiyellerinin araştırılmasıdır. Çalışmada kullanılan mastitis etkenleri toplam 7 bakteri olup, bunlardan 2 tanesi Staphylococcus aureus, diğer 5 bakteri ise KNS’dir. Antibakteriyel aktivite çalışmaları Kirby-Bauer disk difüzyon metodu kullanılarak gerçekleştirilmiştir. Bu çalışma kapsamında yapılan diğer bir antibakteriyel aktivite testi, minimum inhibitör konsantrasyon (MİK) testidir. Antioksidan aktivite çalışmaları 2,2-difenil-1pikrilhidrazil (DPPH) radikal süpürme yöntemi ile belirlenmiştir. Çalışma en geniş inhibisyon zonunun, O. basilicum’un metanol özütünden sağlandığını göstermiştir. Bu çalışmanın sonucu 9 mm’dir. En düşük MİK değeri 3250 µg/mL olarak kayıt edilmiştir. Çalışmada kullanılan O. basilicum, mastitis patojenlerine karşı antibakteriyel aktiviteye sahiptir ve yüksek antioksidan aktivite sulu özütlerden elde edilmiştir (%72). Sonuç olarak, yeni antibakteriyel ve antioksidan ajanların geliştirilmesinde, O. basilicum’ un iyi bir aday olduğunu ortaya koymaktadır.

Antibacterial and Antioxidant Activities of Ocimum basilicum L. Against Mastitis Pathogens

The most common bacterial agents cause inflammation in the mammary gland (mastitis), which disease cause to less yields in cows. These bacteria are Staphylococcus aureus and Coagulase-negative Staphylococci (CNS). Therefore, these bacteria were selected in this study. The purpose of this study is to research the antibacterial and antioxidant potential of the various extracts of Ocimum basilicum L. The mastitis agents used in the study are 7 bacteria in total; 2 of them are Staphylococcus aureus and the other 5 bacteria are CNS. The antibacterial activities studies were carried out using Kirby-Bauer disc diffusion technique. The other antibacterial activity test performed within the scope of this study was minimum inhibitory concentration (MIC) test. Antioxidant activities were determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method. This study showed that the largest inhibition zone was obtained from the methanol extract of O. basilicum. Result of this study is 9 mm. The lowest MIC value was recorded as 3250 μg/mL. It was found that O. basilicum used in the study had antibacterial activity against mastitis pathogens, and high antioxidant activity could be obtained from the water extracts (72%). Consequently, O. basilicum is a good candidate in developing new antibacterial and antioxidant agents.

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