Haydar ALTINOK

Lakkazın poli (Vinil Alkol) -Kalsiyum Aljinat Kürelerine İmmobilizasyonu

Tramates versicolor' dan elde edilen lakkaz enzimi (L), polivinil alkol-kalsiyum aljinat (PVA-CaAlj) kürelerine hapsedildi. Michaelis-Menten sabiti (Km) ve maksimum reaksiyon hızı (Vmax) değerleri sırasıyla serbest enzim için 1.70x10-2 mM ve 2.08x10-3 mM.dak-1 olarak bulundu.İmmobilize enzim için Km ve Vmax değerleri de sırasıyla 2.87x10-2 mM ve 5.30 x 10-3 mM.dak-1 olarak bulundu. Optimum pH değerleri serbest enzim için 5.0 ve immobilize enzim için 6.0 olarak belirlendi. Optimum sıcaklık sırasıyla serbest lakkaz ve immobilize lakkaz için 40oC ve 45oC olarak belirlendi. 4 oC tutulan serbest lakkazın 30 günlük depolama sonrasında orijinal aktivitesinin % 60'ı koruduğu bulunurken aynı koşullarda tutulan immobilize enzimin ise orijinal aktivitesinin% 85'ini koruduğu bulundu. İmmobilize enzimin 10 kez tekrar kullanım sonrasında orijinal aktivitesinin % 75'ini koruduğu bulunmuştur.

Immobilization of Laccase in poly (Vinyl Alcohol)-Calcium Alginate Beads

Laccase enzyme (L) obtained from Tramates versicolor wasentrapped into polyvinyl alcohol–calcium alginate (PVA-CaAlj) beads.Michaelis-Menten constant (Km) and maximum reaction rate (Vmax) values werefound to be 1.70x10-2 mM and 2.08x10-3 mM.min-1for free enzyme respectively. Km and Vmax values were found as 2.87x10-2mM and 5.30 x 10-3 mM.min-1 for entrapped enzymesrespectively. Optimum pH was determined as 5.0 and 6.0 and optimum temperaturedetermined as 40oC and 45oC for free laccase andentrapped laccase respectively. After 30 days of storage at 4 oCfree laccase retained 60 % of its original activity. Also after 30 days ofstorage at 4 oC, entrapped enzymes were retained 85 % its originalactivity. Immobilized enzyme was used repeatedly 10 times, were retained 75% ofits original activities.

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