Hasan KARADAĞ, Cumhur KIRILMIŞ, Emine EROĞLU

Bazı 2-aminotiazol Türevlerine Maruz Kalmış Glutatyon Redüktaz Aktivitesindeki Değişimlerinin Belirlenmesi

Bu çalışmada, 2-Aminotiazol türevleri olan 4,4’-(disulfanediylbis(methylene))bis(thiazol-2-amine) dihyrochloride (DMTA) ve 2-amino-4-(chloromethyl)thiazole hydrochloride (ACT)’in 0 dan 500 mg/L ye değişen derişimlerinin ekmek mayası (Saccharomyces cerevisiae) glutatyon redüktazı (GR) üzerine olan etkileri araştırılmıştır. 25, 50, 100, 250 ve 500 mg/L derişimlere maruz bırakılma ile % GR aktivitesindeki değişimler, DMTA uygulamalarında sırasıyla -5,29 ; -3,85; -2,40 ; -6,73 ve -10,58 olarak hesaplanırken, ACT uygulamalarında sırasıyla +0,98; 0,00; -0,49; -2,45 ve 0,00 olarak hesaplanmıştır. Bu çalışma, DMTA derişimlerinin artışı ile GR aktivitesinde hafif bir düşüş olduğunu ve ACT derişimlerinin artışı ile GR aktivitesinde önemli bir değişimin olmadığını göstermiştir. Ancak kontrol aktivitelerine göre, bu 2-aminotiazol türevlerinin etkileştiği GR aktivitelerinde herhangi bir istatistiksel değişim gözlemlenmemiştir (p > 0,05, n = 3).

Determination of Glutathione Reductase Activity Changes Exposed to Some 2-Aminothiazole Derivatives

In this work, effects of concentrations ranging from 0 to 500 mg/L of some 2-aminothiazole derivatives such as 4,4’-(disulfanediylbis(methylene))bis(thiazol-2-amine) dihyrochloride (DMTA) and 2-amino-4-(chloromethyl)thiazole hydrochloride (ACT) on glutathione reductase from baker's yeast (Saccharomyces cerevisiae) (GR) were investigated. With exposure of 25, 50, 100, 250 and 500 mg/L concentrations, % GR activity changes were calculated as -5.29 ; -3.85 ; -2.40 ; -6.73 and -10.58 in DMTA applications, while these changes were calculated as +0.98 ; 0.00 ; -0.49 ; -2.45 and 0.00 in ACT applications, respectively. This work indicated that there was a slight decrease in GR activity with the increase of DMTA concentrations and there was no significant change in GR activity with the increase of ACT concentrations. But according to control activities, no statitistical changes were observed in GR activities with exposure of these 2-aminothiazole derivatives (p > 0.05, n=3).

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