Sabire YERLİKAYA, Merve ÇİFTÇİ, Hülya ŞEN ARSLAN, Aysun İŞLER

Sarı Soğan (Allium Cepa) Kabuğu Ekstraktının Çiğ, UHT ve Pastörize Sütlere İnoküle Edilen Bazı Patojenler Üzerindeki Antimikrobiyal Etkisinin Belirlenmesi

Bu araştırmada sarı soğan kabuğu ekstraktının (YOPE), pastörize, UHT ve çiğ sütlere inoküle edilen bazı patojenler üzerindeki inhibitör etkisi incelenmiştir. Süt örneklerine %10 (v/v) YOPE ile ilave edilerek 4 ºC’de 5 gün inkübasyona bırakılmıştır. Süt örneklerinin pH ve rengi depolamanın 1, 3 ve 5. günlerinde belirlenmiştir. Ayrıca, süt numunelerine ayrı ayrı 0,0, 5,0 ve 7,0 log kob/mL konsantrasyonlarında E. coli ATCC 25922 ve S. aureus ATCC 25923 inoküle edilmiştir. Bahsedilen numunelerin tümüne ayrı ayrı %0,0 ve 5,0 (v/v) konsantrasyonunda YOPE ilave edilmiştir. YOPE en yüksek inhibitör etkiyi 7,00±0,24 log kob/mL konsantrasyonunda E. coli ile kontamine edilmiş pastörize sütte gösterirken; en düşük inhibitör etkileri 7,00±0,87 log kob/mL konsantrasyonunda S. aureus ile 5,00±0,21 log kob/mL konsantrasyonunda E.coli ile kontamine edilmiş çiğ sütte göstermiştir.

Anahtar Kelimeler:

Antimikrobial aktivite, Süt, E. coli, S. aureus

Determining Antibacterial Effect of Yellow Onion (Allium Cepa) Peel Extract on Some Pathogen Inoculated in Raw, UHT and Pasteurized Milks

In this research, yellow onion peel extract (YOPE) was tested against some pathogens inoculated into pasteurized, UHT and raw milks. Milk samples were added with YOPE at 10% (v/v) and were left at 4 ºC for 5 days. The pH and colour of milk samples were determined at 1, 3 and 5th day of storage. Moreover, the milk samples were inoculated by 0.0, 5.0 and 7.0 log cfu/mL of Escherichia coli ATCC 25922 and by 0.0, 5.0 and 7.0 log cfu/mL of S. aureus ATCC 25923. All the above samples were then added with YOPE at a rate of 0.0 or 5.0% (v/v). YOPE had the greatest inhibitory effect on E. coli inoculated pasteurized milk at a concentration of 7.00±0.24 log cfu/mL, whereas the lowest inhibitory effect was shown on the raw milk inoculated with 7.00±0.87 log cfu/mL S. aureus and 5.00±0.21 log cfu/mL E. coli.

Keywords:

Antibacterial activity, Milk, E. coli, S. aureus,

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