Özlem KARA, Asuman KİLİTÇİ, Gülçin DAĞLIOĞLU

Resveratrolün sıçan böbreğinde cisplatine bağlı hasar üzerindeki koruyucu etkisi

Amaç: Bu çalışmanın amacı, resveratrolün sıçan böbreğinde cisplatin kaynaklı hasar üzerine koruyucu etkisini değerlendirmektir. Gereç ve Yöntem: 30 adet dişi Wistar-Albino sıçan üç gruba ayrıldı: Grup 1'de (kontrol grubu) 3 gün boyunca 1 mL %0.9 NaCl (salin) intraperitoneal olarak uygulandı. Grup 2'de (cisplatin grubu) 3 gün süreyle 7.5 mg/kg intraperitoneal sisplatin verildi. Grup 3'te (cisplatin + resveratrol grubu) 7,5 mg/kg cisplatin ve 10 mg/kg resveratrol intraperitoneal olarak verildi. Sağ böbrekler tüm gruplarda cerrahi olarak ekstirpe edildi. Hem kanda hem de dokularda malondialdehit (MDA) seviyeleri ve katalaz (CAT) ve süperoksit dismutaz (SOD) aktiviteleri ölçüldü. Ayrıca böbrek dokusundan hazırlanan slaytlar ışık mikroskobu ve immünohistokimya ile incelenerek damar tıkanıklığı, kanama, tübül dejenerasyonu ve glomerüler hasar gibi toksisite belirteçleri değerlendirildi. Bulgular: Grup 2'de doku hasarı diğer gruplara göre anlamlı derecede yüksekti. Grup 2'de diğer gruplara göre MDA düzeyleri anlamlı olarak yüksek, SOD ve CAT aktiviteleri daha düşüktü. Sonuç: Kısa dönem bulgularımıza göre resveratrol sıçan böbreğinde cisplatinin zararlı etkisini önlemede etkili bir molekül olabilir.

Protective effect of resveratrol on cisplatin induced damage in rat kidney

Purpose: The aim of this study was to evaluate the protective effect of resveratrol on cisplatin induced damage in rat kidney. Materials and Methods: 30 female Wistar-Albino rats were allocated to form three groups: In group 1 (control group), 1 mL of 0.9% NaCl (saline) was administered intraperitoneally for 3 days. In group 2 (cisplatin group), 7.5 mg / kg intraperitoneal cisplatin was given for 3 days. In group 3 (cisplatin + resveratrol group) 7.5 mg / kg cisplatin and 10 mg / kg resveratrol were given via intraperitoneal route. Right kidneys were surgically extirpated in all groups. Malondialdehyde (MDA) levels and activities of catalase (CAT) and superoxide dismutase (SOD) were measured in both blood and tissues. Also, toxicity markers such as vascular congestion, hemorrhage, tubule degeneration and glomerular damage were assessed by examining the slides prepared from kidney tissue with microscopy. Results: Tissue damage was significantly higher in group 2 than other groups. The MDA levels were significantly higher and the activities of SOD, and CAT were lower in group 2 than other groups. Conclusion: According to our short term findings, resveratrol might be an effective molecule to prevent the harmful effect of cisplatin in rat kidney.

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