Solmaz SUSAM, Nevin İLHAN, Fatma TEDİK, Buket BERK, Ayşe Şebnem İLHAN

Etanol ile indüklenmiş akut gastrik ülser modelinde tangeretin ve pomegrenatın ayrı ayrı ve kombinasyon halinde koruyucu etkisi

Amaç: Bu çalışma, "Akut gastrik ülserinden korunma konusunda pomegrenat ve tangeretinin ayrı ayrı veya birlikte kullanılması yararlı mıdır?" sorusuna cevap bulmak amacıyla tasarlanmıştır. Gereç ve Yöntem: Mide dokusunda tangeretin and pomegranatın gastroprotektif etkisi, seçili inflamatuar sitokinler [tümör nekroz faktörü-α (TNF-α), interlökin-6 (IL-6), IL-1β ve IL-10)], lipid peroksit düzeyleri ve enzimatik antioksidan aktiviteleri ölçülerek değerlendirildi. Bulgular: Tüm gruplar kontrol, gastrik ülser, POM+EtOH, TAN+EtOH ve POM+TAN+EtOH şeklinde sırasıyla yazıldığında; IL-1β sitokin düzeyleri: 0,147, 0,24, 0,228, 0,195 ve 0,182 pg/g protein olarak ölçüldü. IL-6 düzeyleri; 16,857, 25,923, 19,797, 18,838 ve 17,896 pg/ mg protein idi. TNF-α düzeyleri 39,916, 49,97, 44,678, 41,673 ve 40,844 pg/mg protein idi. Son olarak IL-10 düzeyleri ise 33,496, 28,071, 29,693, 30,073 ve 30,008 pg/mg protein olarak ölçüldü. SOD aktiviteleri 18,038, 13,731, 15,506, 14,439, 15,943 olarak tayin edildi. CAT aktiviteleri ise 674,638, 639,964, 673,382, 664,691, 671,203 idi. Protein karbonil düzeyleri 26,799, 40,30, 33,052, 34,579 ve 32,79 olarak ölçüldü. Son olarak MDA düzeyleri ise 5,239, 9,814, 6,695, 5,771 ve 5,836 olarak bulundu. Kısaca; POM ve TAN, antioksidan fonksiyonlarını malondialdehit (MDA) ve protein karbonil düzeylerini düşürerek ve süperoksit dismutaz (SOD) ve katalaz (CAT) aktivitesini artırarak gösterdiler. Ayrıca bu ajanlar anti-inflamatuar etkilerini TNF-α, IL-6 ve IL-1β düzeylerini azaltarak ve IL-10 düzeylerini yükselterek sergilemişlerdir. Sonuç: Tangeretin ve pomegrenatın etanol kaynaklı akut gastrik ülserine karşı potansiyel bir gastroprotektif etkiye sahip olduğu ve kombine tedavinin tek başına POM veya TAN'ın etkisinden daha faydalı olduğunu göstermektedir. Ayrıca bu etkinin, seçilen her iki flavonoidin sinerjistik etki göstererek inflamasyon parametrelerinin düzeylerini azaltabilmesi ve antioksidan düzeylerini yükseltebilmesinden kaynaklandığı düşünülmektedir.

Anahtar Kelimeler:

Mide ülseri, pomegranate, tangeretin, etanol, rat

The protective effect of tangeretin and pomegranate separately and in combination on ethanol-induced acute gastric ulcer model

Purpose: This study was designed to find an answer to the question, "Is it beneficial to use pomegranate (POM) and tangeretin (TAN) separately or in combination, for the prevention of acute gastric ulcer?". Materials and Methods: The gastroprotective effect of tangeretin and pomegranate was determined by measuring the levels of the selected inflammatory cytokines [tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), IL-1β and IL-10], lipid peroxides, and enzymatic activities of antioxidants in gastric tissue samples. Results: When all groups are written as control, gastric ulcer, POM+EtOH, TAN+EtOH and POM+TAN+EtOH, respectively; IL-1β cytokine levels were measured as 0.147, 0.24, 0.228, 0.195 and 0.182 pg/g protein. IL-6 levels; 16,857, 25,923, 19,797, 18,838 and 17,896 pg/mg protein. TNF-α levels were 39,916, 49.97, 44,678, 41,673 and 40,844 pg/mg protein. Finally, IL-10 levels were measured as 33,496, 28,071, 29,693, 30,073 and 30,008 pg/mg protein. SOD activities were determined as 18,038, 13,731, 15,506, 14,439, and 15,943. CAT activities were 674,638, 639,964, 673,382, 664,691, and 671,203. Protein carbonyl levels were measured as 26,799, 40,30, 33,052, 34,579 and 32,79. Finally, MDA levels were found as 5,239, 9,814, 6,695, 5,771 and 5,836. Briefly, POM and TAN showed their antioxidant functions by decreasing the levels of malondialdehyde (MDA), and protein carbonyl and increasing the activity of superoxide dismutase (SOD) and catalase (CAT). And also, these protective agents exhibited their anti-inflammatory functions by decreasing the content of TNF-α, IL-6, and IL-1β, and increasing the IL-10 levels. Conclusion: Tangeretin and pomegrenate have a potential gastroprotective effect against ethanol-induced acute gastric ulcer and that the combined treatment is more beneficial than the effect of POM or TAN alone. In addition, this effect is thought to be due to the fact that both selected flavonoids can show a synergistic effect, reducing the levels of inflammation parameters and increasing antioxidant levels.

Keywords:

Gastric ulcer, pomegranate, tangeretin, ethanol; rat,

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