Epibrassinolid, Gibberellik Asit ve Naftalen Asetik Asittin Bazı Nar Çeşitlerinde Çiçek Tozu Çimlenme Oranlarına Etkisi

Bu araştırma, Çanakkale Onsekiz Mart Üniversitesi Dardanos Yerleşkesi içerisindeki nar koleksiyon bahçesinde bulunan ‘Caner I’, ‘Caner II’ çeşitleri ve ‘26–3’ nar tipinde yürütülmüştür. Bitki büyüme düzenleyicilerden epibrassinolid (Epi–B1) 0.001, 0.01, 0.1 ppm, gibberellik asit (GA3) 25, 50, 100 ppm ve naftalen asetik asit (NAA) 0,5, 1,0 ve 2,5 ppm konsantrasyonlarında çiçek tozlarını çimlendirme ortamına uygulanmıştır. Çiçek tozu çimlenme denemeleri in vitro koşullarda ‘agar–petri’ yöntemi ile yapılmıştır. ‘Caner I’, ‘Caner II’ çeşitleri ve ‘26–3’ tipinde bulunan erkek (steril) ve hermafrodit (fertil) çiçek tiplerinin oranları saptanmıştır. Uygulanan büyümeyi düzenleyicilerin çiçek tozlarının çimlenme oranlarına etkisi her iki çiçek tipinde de belirlenmiştir. İncelenen nar tip ve çeşitlerinde yaklaşık olarak %75 steril çiçek ve %25 oranında fertil çiçek belirlenmiştir. En yüksek çiçek tozu çimlenme oranları epibrassinolid uygulamasının 0,1 ppm konsantrasyonunda elde edilmiştir

Anahtar Kelimeler:

Punica granatum, Epibrassinolid, Gibberellik asit, NAA, Polen çimlenme

Effect of Epibrassinolide, Gibberellic acid and Naphthalene Acetic Acid on Pollen Germination of Some Pomegranate Cultivars

This research was carried out on ‘Caner I’, ‘Caner II’ cultivars and type ‘26–3’ grown at the pomegranate collection orchard located at Dardanos campus of Çanakkale Onsekiz Mart University, Çanakkale, Turkey. Plant growth regulators, epibrassinolide (Epi–B1) at 0.001, 0.01 and 0.1 ppm, gibberellic acid (GA3) at 25, 50 and 100 ppm and naphthalene acetic acid (NAA) at 0.5, 1.0 and 2.5 ppm concentrations were applied to the pollen germination medium. Pollen germination experiments were done using agar in the petri method. Ratios of male (sterile) and hermaphrodite (fertile) flower types were determined on ‘Caner I’, ‘Caner II’ cultivars and type ‘26–3’. Effects of the plant growth regulators on pollen germination ratios were assessed on both types of flowers. Approximately, 25%hermaphrodite and 75%male flowers were found on the cultivars and the type. The highest pollen germination percentages were obtained from the 0.1 ppm Epi–Bl application

Keywords:

Punica granatum, Epibrassinolide, Gibberellic acid, NAA, Pollen germination,

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Engin, H., Hepaksoy, S., 2003. Bazı nar çeşitlerinin çiçek tozu çimlenme güçlerinin belirlenmesi. Ege Üniv. Ziraat Fak. Derg. 40 (3): 9–16.
Eti, S., 1991. Bazı meyve tür ve çeşitlerinde değişik in vitro testler yardımıyla çiçek tozu canlılık ve çimlenme yeteneklerinin belirlenmesi. Ç.Ü. Ziraat Fakültesi Dergisi. 6 (1): 69–80.
Gökbayrak, Z., Engin, H., 2015. Effect of plant growth regulators on enhancing in vitro pollen germination in grapevine cultivars. 3rd Balkan Symposium on Fruit Growing, 15–18 Eylül 2015. Belgrad, Sırbistan.
Gözlekçi, S., Kaynak, L., 2000. Investigations on pollen production and quality in some standards pomegranate (Punica granatum L.) cultivars. Options No: 42: 71–78.
Grove, M.D., Spencer, G.F., Rohwedder, W.K., Mandava, N.B., Worley, J.F, Warthen, J.D., Steffens, G.L., Flippen–Anderson, J.L., Cook Jr., J.C., 1979. Brassinolide, a plant growth promoting steroid isolated from Brassica napus pollen. Nature. 281: 216–217.
Hewitt, F.R., Hough, T., O’Neill, P., Sasse, J.M., Williams, E.G., Rowon, K.S., 1985. Effect of brassinolide and other growth regulators on the germination and growth of pollen tubes of Prunus avium using a multiple hanging–drop assay. Aust. J. Plant Physiol. 12: 201–211.
Imani, A., Nazarian, M., 2013. Effects of boron and growth regulators on germination of Punica granatum pollen grains. Advanced Crop Science. 3 (4): 268–272.
Josan, J.S., Jawanda, J.S., Uppal, D.P., 1980. Studies on the floral biology of pomegranate. II. Anthesis, dehiscence, polen studies and receptivity of stigma. Punjab Horticultural Journal. 19 (1–2): 66–70.
Kang, Y.Y., Guo, S.R., 2011. Role of brassinosteroids on horticultural crops. In: Brassinosteroids: A Class of Plant Hormone. 269–288.
O’Kelly, J.C., 1955. External carbohydrates in growth and respiration of pollen tubes “in vitro”. American Journal of Botany. 42 (3): 322–326.
Reig, C., Mesejo, C., Martínez–Fuentes, A., Agustí, M., 2014. Naphthaleneacetic acid impairs ovule fertilization and early embryo development in loquat (Eriobotrya japonica Lindl.). Scientia Horticulturae. 165: 246–251.
Sharma, C.M., Gaur, R.D., 1982. Studies on morphology, germination and viability of pomegranate (Punica granatum L.) pollen. Journal of Palynology. 20 (2): 87–92.
Shulman, Y., Fain Berstein, L., Lavee, S., 1984. Pomegranate fruit development and maturation. Journal of Horticultural Science. 59 (2): 265–274.
Singh, I., Shono, M., 2003. Effect of 24–epibrassinolide on pollen viability during heat–stress in tomato. Indian J. Exp. Bot. 41: 174–176.
Taylor, P.E., Spuck, K., Smith P.M., Sasse, J.M., Yokota, T., Gritfiths, P.G., Cameron, D.W. 1993. Detection of brassinosteroids in pollen of Lolium perenne L. by immunocytochemistry. Planta. 189: 91–100.
Tosun, F., Koyuncu, F., 2007. Kirazlarda (Prunus avium L.) çiçek tozu çimlenmesi ve çiçek tozu çim borusu gelişimi üzerine bazı kimyasal uygulamaların etkileri. Akdeniz Üniversitesi Ziraat Fakültesi Dergisi. 20 (2): 219–224.
Varasteh, F., Arzani, K., 2009. Classification of some Iranian pomegranate (Punica granatum) cultivars by pollen morphology using scanning electron microscopy. Hort. Environ. Biotechnol. 50 (1): 24–30.
Vasil, I.K., 1964. Effect of boron on pollen germination and pollen tube growth. In: Pollen Physiology and Fertilization, Linkens, H.F. (ed.) North–Holland publishing Company, Amsterdam. 107–119.
Viti, R., Bartolini–Vitagliano, C., 1990. Growth regulators on pollen germination in olive. Acta Hort. 286: 227–230.
Voyiatzsis, D.G., Paraskevopoulou–Paroussi, G., 2005. Factors affecting the quality and in vitro germination capacity of strawberry pollen. International Journal of Fruit Science. 5 (2): 25–35.
Wetzstein, H.Y., Ravid, N., Wilkins, E., Martinelli, A.P., 2011. A morphological and histological characterization of bisexual and male flower types in pomegranate J. Amer. Soc. Hort. Sci. 136 (2): 83–92.
Xue, X.W.J., Zhang, A.L.C., 2008. Effects of plant growth regulating substances on pollen germination and tube growth in Chaohong peach. Journal of Northwest A & F University (Natural Science Edition), 2008–04.
Zhou, R., Zhang, C., 2010. Effect of gibberellin and paclobutrazol on pollen germination and tube growth in pear. Journal of Henan Institute of Science and Technology (Natural Science Edition), 2010–02.