Kumbirai Deon MANDEBERE, F. Seyma GOKDEMIR, Gonul SOLMAZ, Sumer ARAS

COMPARISON OF TOXIC AND ANTITOXIC EFFECTS OF CISPLATIN AND CAMP, AT CYP1A1 GENE EXPRESSION LEVEL OF WISTAR ALBINO TYPE RAT LIVER

Öz Throughout the course of cancer treatment, cisplatin has proven to be asuccessful chemotherapy agent. Several researches have been conducted in vivo and/or invitro on gene expression changes occurring with the impact of this agent. The current studyfocuses on the alterations in CYP1A1 gene expression levels in liver cells by cisplatin andcAMP treatment. CYP1A1 gene which the expression levels were analyzed, encodes amember of the cytochrome P450 superfamily of enzymes. Throughout the study, livertissues from Wistar-type albino rat, also known as the laboratory rat, were used. Fornormalization of the data, the housekeeping gene TATA box binding protein (TBP) waspreferred. The results showed that; gene expression was reduced in tissues subjectedindependently to cisplatin and cAMP, however the tissues treated with cAMP showedfurther reduction in gene expression levels. In contrast, tissues subjected to both cisplatinand cAMP showed an increase in the level of expression. This study aims to provide analternative perspective for comparative toxicology cases and will also serve as a guide forelucidation of cases connected with forensic toxicology.

Anahtar Kelimeler:

cisplatin, cAMP, Wistar albino, cytochrome p450, gene expression, forensic toxicology

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