Hazal İPEKÇİ, Tuğba Tunalı AKBAY, Göksel ŞENER

Sulu Sarımsak Ekstresinin Deneysel Sepsis Modelinde Böbrek Hasarı Üzerine Etkisi

Amaç: Sepsis, patojenlere veya onların salgıladığı maddelere karşı verilen sistemik inflamatuvar bir yanıttır. Bu çalışmada sepsiste oluşan böbrek hasarına karşı sulu sarımsak ekstresinin olası koruyucu etkisi incelenmiştir. Yöntemler: Çalışmamızda sıçanlar; ‘kontrol’, ‘sepsis’, ‘sepsis+sarımsak’ ve ‘sepsis+ön-tedavili sarımsak’ grupları olmak üzere 4 gruba ayrılmıştır. Sepsis modeli çekal ligasyon ve perforasyon yöntemi ile oluşturulmuştur. ‘Sepsis+ön-tedavili sarımsak’ grubuna sepsis oluşumundan 15 gün önce başlanarak, 250 mg/kg/gün dozunda sulu sarımsak ekstresi oral yoldan uygulanmıştır. ‘Sepsis+sarımsak’ grubunda ise tedavi sepsis uygulamasından hemen sonra tek doz (250 mg/kg) olarak yapılmıştır. Sepsis oluşumundan 12 saat sonra bütün gruplardaki sıçanlar dekapite edilerek, böbrek dokuları alınmıştır. Böbrek dokusunda, glutatyon (GSH) ve malondialdehit (MDA) seviyeleri, süperoksit dismutaz (SOD), doku faktörü (TF), katalaz (CAT) ve miyeloperoksidaz (MPO) aktivitesi tayin edilmiştir. Bulgular: Sepsise bağlı olarak böbrek dokusunda artan MDA düzeyleri ve MPO aktivitesi ile azalan GSH düzeyleri ve SOD ve CAT aktiviteleri sulu sarımsak ekstresi ile geri çevrilmiştir. TF aktivitesi sepsiste değişmemiştir. Kı- salmış pıhtı oluşum zamanı, artmış TF aktivitesini göstermektedir. Bu doğ- rultuda ön-tedavili sepsis sarımsak grubunda TF aktivitesi artmıştır. Sonuç: Sulu sarımsak ekstresi kullanımının yeni sepsis tedavi yöntemleri geliştirilirken oksidan-antioksidan dengesi açısından dikkate alınması gerektiğini düşünmekteyiz.

Anahtar Kelimeler:

Sepsis, böbrek, sulu sarımsak ekstresi, oksidatif stres, doku faktörü

Effect of an Aqueous Garlic Extract on Kidney Damage in an Experimental Model of Sepsis

Objective: Sepsis is a systemic inflammatory response against pathogens or substances secreted by pathogens. In this study, the potential protective effect of an aqueous garlic extract (AGE) against sepsis-induced kidney injury. Methods: Rats were divided into four groups: control, sepsis, sepsis+AGEgarlic, and sepsis+pretreated garlic. Sepsis was induced using cecal ligation and perforation. An AGE was orally administered to rats in the sepsis+pretreated garlic group at a dose of 250 (mg/kg/day) for 15 days prior to sepsis induction. In rats in the sepsis+garlic group, the AGE was administered at a single dose (250 mg/kg) immediately after sepsis induction. Twelve hours after sepsis induction, all rats were decapitated and kidney tissues were taken. Glutathione (GSH) and malondialdehyde (MDA) levels and superoxide dismutase (SOD), tissue factor (TF), catalase (CAT), and myeloperoxidase (MPO) activities were determined in the kidney issue. Results: Increased MDA levels and MPO activity and decreased GSH level and SOD and CAT activities due to sepsis were reversed by the AGE. TF activity did not change in sepsis. Shortened clot formation time shows increased TF activity. Accordingly, kidney TF activity significantly increased in mice in the pre-treated garlic group. Conclusion: AGE usage should be considered in developing new sepsis treatment strategies in terms of oxidant and antioxidant balance.

Keywords:

Sepsis, kidney, aqueous garlic extract, oxidative stress, tissue factor,

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