Ayşe Yılmaz, Wafi ATTAALLAH, Gökhan Biçim, Evren Uçar, Nazlı Arda, A. Özdemir Aktan, A. Suha Yalçın

Deneysel meme kanseri modelinde süt proteinlerinin koruyucu etkisi

Amaç: Süt serumu proteinlerinin insan sağlığına üzerine birçok yararlı etkileri olduğu bilinmektedir. Çalışmamızda, sıçanlarda N-metil-N-nitrozure ile indüklenen meme kanseri modelinde süt serumu proteinlerinin koruyucu etkileri araştırılmıştır.Yöntemler: Çalışmamızda deneysel meme kanseri modeli oluşturmak üzere 130 günlük Sprague-Dawley türü, dişi, virjin sıçanlara 100 gün ara ile iki kez N-metil-N-nitrozüre enjekte edildi. Çalışma boyunca sıçanlara haftada iki kez orogastrik gavajla süt serumu proteinleri verildi. Sıçanlar 180 gün sonunda sakrifiye edildi doku örnekleri toplandı. Karaciğer, böbrek ve meme dokularındaki glutatyon, malondialdehit ve protein karbonil değerleri karşılaştırıldı.Bulgular: Tüm dokularda en yüksek malondialdehit ve protein karbonil değerleri N-metil-N-nitrozüre grubundaki sıçanlarda en düşük malondialdehit ve protein karbonil değerleri de kontrol grubunda gözlendi. N-metil-N-nitrozüre ile birlikte süt serumu proteinleri verildiğinde ise bu değerlerdeki artışların baskılanarak kontrol grubu değerlerine yaklaştığı gözlendi.Sonuç: Çalışmamızda N-metil-N-nitrozüre ile oluşturulan meme kanseri modelinde N-metil-N-nitrozüre etkisiyle oluşan biyokimyasal değişikliklere karşı süt serumu proteinlerinin koruyucu etkisi olduğu gözlenmiştir.Anahtar Kelimeler: Süt serumu proteinleri, deneysel meme kanseri, N-metil-N-nitrozüre

Anahtar Kelimeler:

Süt serumu proteinleri, deneysel meme kanseri, N-metil-N-nitrozüre

Protective effect of whey proteins in experimental breast cancer model

Objective: It is known that whey proteins have a number of effects on maintaining human health. In this study protective effect of whey proteins in N-methyl-N-nitrosourea induced breast cancer model was investigated.Methods: In our study, female, virgin, 130 days old Sprague- Dawley rats were used to form a cancer model. N-methyl-Nnitrosourea (50 mg/kg) was injected two times by a 100 days break. During the study, whey proteins were given to the rats by orogastric gavage 2 times a week. At the end of 180 days, rats were sacrificed and samples were obtained. Glutathione, malondialdehyde and protein carbonyls levels were determined in liver, kidney and breast tissues.Results: Among all groups, the highest malondialdehyde and protein carbonyl levels were detected in the N-methyl-Nnitrosourea group. The increase in these values were reversed back by whey proteins.Conclusion: The protective role of whey proteins against biochemical changes were observed in N-methyl-N-nitrosourea induced breast cancer model.

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