Murat BAŞAR, Tülay İREZ, Oktay ARDA, Mehmet TÜRKER

Süperovulasyon protokolünde kullanılan GnRH agonistinin oosit olgunluğu ve çapına etkileri

Amaç: Bu çalışmada FSH ve hCG kullanılarak süperovule edilen farelerde GnRHa’nın ovaryum morfolojisi, oosit çapı, sayısı ve olgunluğuna etkilerinin incelenmesi amaçlanmıştır. Yöntem: Çalışmada, 3 aylık (25-30 gr), dişi BALB/C fareler kullanılmış ve kontrol, süperovulasayon protokolü uygulanmış ve süperovulasyon protokolünden önce GnRHa uygulanmış grup olmak üzere 3 gruba ayrılmıştır (n=10/grup). Kontrol grubundaki farelere i.p. olarak %0.09’luk NaCl enjeksiyonu uygulanmıştır. Süperovulasyon, 5 IU FSH (i.p.) ve bu enjeksiyondan 48 saat sonra hCG (i.p.) enjeksiyonu uygulanarak sağlanmıştır.Bulgular: Süperovülasyondan önce GnRHa ile muamele edilen gruptaki farelere süperovulasyon uygulamasından 24 saat önce GnRHa (20 mg/kg) i.m. olarak uygulanmıştır. Fareler, hCG enjeksiyonundan 13 saat sonra servikal dislokasyon yöntemiyle sakrifiye edilmişlerdir. Ovaryumlar ve tuba uterinalar histolojik inceleme için alınmıştır. Sonuç: Önceden GnRHa ile muamele edilen grupta, kontrol ve süperovulasyon gruplarına göre oosit sayısı, oosit olgunluğu, ovulasyon oranı ve oosit çapları istatistiksel olarak anlamlı derecede yüksektir (p

Morphological effect of different superovulation protocols to the mouse ovary

Objectives: The objective of this study was to evaluate the effects of GnRHa on; ovarian morphology, oocyte diameter, number and maturation of oocytes in superovulated mice with FSH and hCG. Methods: Three month old BALB/C female mice(weight: 25-30 g) were assigned to 3 experimental groups, which were: control, superovulated and superovulated with GnRHa pretreatment (n=10 per group). Control mice had an i.p. injection of 0.1 ml 0.9% NaCl.Results: Superovulation induced with 5 IU FSH (i.p.) and 5 IU hCG (i.p.) 48 hours later. Mice in the superovulated with GnRHa pretreatment group received GnRHa (20 mg/kg) 24 hours before superovulation. Mice were sacrifice by cervical dislocation thirteen hours after hCG administration. The ovaries and oviducts harvested for histological assessment. Conclusion: The oocyte numbers and their maturation, ovulation rate, and the diameters of the oocytes were higher in GnRHa pretreated group than the control and superovulated ones (p

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