Selmin TOPLAN, Birsen AYDEMİR, Ali Rıza KIZILER, Bülent ALICI, İlhan ONARAN, Abdullah AKSU

İdiyopatik infertil erkeklerde antioksidan sistem ile semen parametreleri arasındaki ilişkiler

Amaç: İdiyopatik infertilitede antioksidan savunma sistemlerine ait çeşitli bileşenlerin rolü araştırılmış olmakla birlikte, bu bileşenlerin aynı hasta grubu içerisinde birlikte değerlendirildiği bir çalışmaya rastlanamamıştır. Çalışmanın amacı idiyopatik infertil hasta ve fertil kontrol gruplarında seminal plazma selenyum, redükte glutatyon (GSH), katalaz (CAT) ve süperoksit dismutaz (SOD) aktivitelerinin ölçümü ve semen parametrelerinin (sperm sayımı, motilite ve morfoloji) analizi yapılarak birbirleri ile olan ilişkileri her iki grupta karşılaştırmaktır.Yöntem: Çalışma grupları 28 kişilik idiyopatik infertil ile 30 kişilik fertil kontrol gruplarından oluşturulmuştur. Semen analizleri Dünya Sağlık Örgütü rehberine göre sperm sayımı, motilite ve morfolojisi değerlendirilmiştir. Seminal plazma SOD ve CAT aktiviteleri, GSH konsantrasyonları biyokimyasal yöntemler ile ölçülmüştür. Seminal plazma selenyum düzeyleri ise atomik absorpsiyon spektrofotometresi ile belirlenmiştir.Bulgular: Bulgularımızda sperm sayısının idiyopatik infertil grubunda fertil kontrol grubuna göre anlamlı olarak düşük saptanmıştır (p

Relationships between the semen parameters and the antioxidant system in idiopathic infertile patients

Objectives: Although it has been addressed that the decreases in levels of various antioxidant system components such as selenium, reduced glutathione (GSH), catalase (CAT) and superoxide dismutase (SOD) play a role in idiopathic infertility, there is not any report concerning altogether evaluation of these components in same study. The aim of the present study was to determine seminal plasma levels of selenium and GSH, activities of CAT and SOD and sperm parameters in men with idiopathic infertile compared with fertile males, and to evaluate the relationships among these parameters.Methods: Twenty-eight men with idiopathic infertility and 30 healthy fertile men were recruited to this study. Semen analysis (sperm count, motility, morphology) was performed according to the World Health Organization (WHO) guidelines to obtain sperm concentration, motility, and morphology. Activities of SOD and CAT and GSH levels in seminal plasma were measured by biochemical methods. Selenium levels in seminal plasma were determined by atomic absorption spectrophotometer.Results: The sperm count in the idiopathic infertile male group were found lower than those in fertile male group (p

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