Deneysel diyabetik nefropatide ırbesartan'ın apoptoz üzerine koruyucu etkileri
Amaç: Bu çalışmada, Angiotensin II tip 1 reseptör blokeri olan irbesartan'ın Streptozotocin (STZ)-diyabetik sıçanlarda apoptoz ve apoptozu düzenleyen antiapoptotik (Bcl-2) proteini üzerine etkileri araştırıldı. Yöntem: Çalışmada 24 adet erkek Wistar tipi albino sıçandan oluşan 3 grup kullanıldı. I.grup; sağlıklı kontrol, 2.grup; tedavisiz STZ-diyabetik(60 mg/kg, tek doz, i.p), 3-grup; irbesartan(15 mg/kg/gün, intragastric 4 hafta) uygulanan STZ-diyabetik sıçan grubu olarak düzenlendi. Deney süresince tüm gruplardaki sıçanların kan glukoz ve mikroalbuminüri düzeyleri ölçüldü. Deney sonunda alınan böbrek dokuları nötral formalde tespit edilip parafine gömüldü. Böbrek doku kesitlerine apoptoz tespiti amacıyla TÜNEL metodu uygulandı. Bcl-2 antikoru kullanılarak immunohistokimyasal boyama yapıldı. Bulgular: İrbesartan uygulanan diyabetik grupta mikroalbuminüri düzeyleri tedavisiz diyabetik grupa kıyasla anlamlı olarak azaldı (p
Protective effects of irbesartan on apoptosis in experimental diabetic nephropathy
Objectives: The aim of this study is to investigate effects of irbesartan as an Ang II type I blocker on apoptosis and anti-apoptotic protein Bcl-2 in the streptozotocin (STZ)-induced diabetic rat. Methods: 24 male Wistar albino rats were used for three groups. The first group was the non-diabetic control group. Second group was the untreated STZ-induced diabetic group, (60 mg/kg, single dose, i.p). The third group was irbesartan treated (15 mg/kg/day, intragastric, for 4 week) STZ-diabetic rats. During the period of the experiment, blood glucose and microalbuminuria levels of the rats were measured. At the end of the study renal tissue samples were fixed in neutral formalin and embedded in paraffin. Tissue sections were examined for apopto¬sis by TUNEL method and for anti-apoptotic protein Bcl-2 by immunohistochemical staining. Results: The microalbuminuria levels of the irbesartan treated diabetic: group were found reduced when compared with the untreated dia¬betic group (p
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- ISSN: 1300-5227
- Başlangıç: 1998
- Yayıncı: -
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