Fazilet UĞUR DUMAN, Gizem AKKAŞ AKGÜN, Hasan AYDEDE, Semin AYHAN, Peyker TEMİZ

Tiroid papiller karsinom, papiller mikrokarsinom ve tiroid papiller karsinomun lenf nodu metastazında apoptoz ve hücre siklusu ile ilişkili belirleyicilerin (p16, p2l, p27, p53, bcl-2, bax, bcl-xl ve siklin-D1) doku microarray yöntemiyle saptanması

Giriş ve Amaç: Bu çalışmanın amacı, tiroid papiller karsinom ve lenf nodu metastazının patogenezinde apoptoz ve hücre döngüsü ile ilişkili gen ürünlerinin rolünü belirlemektir. Gereç ve Yöntemler: Tiroid papiller karsinom (n = 35), tiroid papiller mikrokarsinom (TPMK) (n = 22), TPK-lenf nodu metastazı (TPK-LNM) (n = 12) ve adenomatöz nodülde (AN) ( n = 20) doku mikroarray yöntemi (TMA) kullanılarak p16, p21, p27, p53, bcl-2, bax, bcl-xL ve siklin D1'in immünohistokimyasal olarak boyanması incelendi. Bulgular: AN'lerin Bcl-2 boyaması, malign gruplarınkilerden önemli ölçüde farklıydı. p53, p16, p21 boyama yüzdeleri, malign gruplarda benign lezyonlara göre anlamlı olarak daha yüksekti. TPC-LNM grubu, primer tümör gruplarından daha yüksek p16 ve siklin D1 pozitifliğine sahipti. p27 boyanmasındaki en dikkat çekici fark , TPK-LNM ve TPK grupları arasındaydı. Sonuç: Hücre döngüsü düzenleyicilerinin, özellikle bcl-2 ailesinin, TPC karsinogenezinde önemli roller oynadığı sonucuna vardık. Siklin-CDK kompleksi (p16, p21, p27) üzerinde etkili olan sikline bağımlı kinaz inhibitörleri, malignite, progresyon ve kötü prognoz potansiyeli ile daha çok ilişkiliydi. p53, hem apoptozu hem de hücre döngüsünü düzenleyen proteinlerle etkileşime girerek TPC patogenezinde önemli bir rol oynar.

Anahtar Kelimeler:

tiroid papiller karsinom, , p16, , p21, , p27, , p53, , bcl-2, , bax, , bcl-xl, , siklinD1,

Microarray analysis of cell cycle and apoptosis biomarkers (p16, p21, p27, p53, Bcl-2, Bax, Bcl-xL and Cyclin D1) in thyroid papillary carcinoma, papillary microcarcinoma and lymph node metastasis of thyroid papillary carcinoma

AObjective: The aim of this study was to identify the role of apoptosis and cell cycle associated gene products in the pathogenesis of thyroid papillary carcinoma (TPC) and its lymph node metastasis. Materials and Methods: Cases of thyroid papillary carcinoma (n=35), thyroid papillary microcarcinoma (TPMC) (n=22), TPC-lymph node metastasis (TPC-LNM) (n=12), and adenomatous nodule (AN) (n=20) were examined using tissue microarray method (TMA) by immunohistochemistry staining for p16, p21, p27, p53, bcl-2, bax, bcl-xL and cyclin D1. Results: Bcl-2 staining of the ANs was significantly differed from those of malignant groups. p53, p16, p21 staining percentages were significantly higher in the malignant groups than in the benign lesions. TPC-LNM group had higher p16 and cyclin D1 positivity than the primary tumor groups. The most remarkable difference of p27 staining was between the TPC-LNM and TPC groups. Conclusion: We concluded that cell cycle regulators, especially bcl-2 family, play important roles in TPC carcinogenesis. The cyclin-dependent kinase inhibitors acting on the cyclin-CDK complex (p16, p21, p27) were more associated with potential for malignancy, progression and poor prognosis. p53 plays an important role in the TPC pathogenesis by interacting with the proteins regulating both apoptosis and the cell cycle.

Keywords:

thyroid papillary carcinoma, , p16, p21, p27, p53, bcl2, bax, bclXl, cyclinD1,

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