Şadiye GÜNPINAR, Olgu ALPERAT KILIÇ, Mehmet TOSUN, Tülin FIRAT

Palatal Mukozal Hasar Oluşturulan Tavşanlarda Profilaktik Hypericum Perforatum Yağ Uygulamasının Antioksidan Özellikleri

Amaç: Bu çalışmada, topikal Hypericum perforatum (HP) yağının tavşanlarda profilaktik olarak uygulanmasının antioksidan ve yumuşak doku yara iyileştirici özelliklerini belirlemek amaçlandı. Gereç ve Yöntem: Otuz altı Yeni Zelanda albino tavşanı rastgele p-HP yağı (test, n = 18) ve p-zeytin yağı (kontrol, n = 18) gruplarına ayrıldı. Her bir grup, cerrahi öncesi topikal olarak HP veya zeytinyağı ile ön işleme tabi tutuldu. Dişeti biyopsileri alınarak re-epitelizasyon (RE) ve granülasyon dokusu olgunlaşması (GTM) açısından analiz edildi. Klinik görünüm cerrahi sonrası 3., 7. ve 14. günlerde eritem, epitel birleşimi ve genel yara görünümü açısından skorlandı. VEGF ve FGF-2 seviyeleri immünohistokimyasal olarak değerlendirildi. Doku katalaz (CAT) ve malondialdehit (MDA) seviyeleri ELISA yöntemi ile ölçüldü.Bulgular: Epitelyal birleşme (7. günde) ve genel yara görünüm skorlarının (7. ve 14. günlerde) p-HP yağı grubunda, p-zeytinyağı grubundakinden daha yüksek olduğu tespit edildi (p <0.05). Diğer taraftan, RE skorları gruplar arasında farklılık göstermedi (p> 0.05). Pozitif olarak boyanmış FGF-2 hücrelerinin sayısı test grubunda kontrol grubundan daha yüksekti (p <0.05), fakat gruplar arasında pozitif boyanmış VEGF damar sayısı açısından anlamlı bir farklılık yoktu. p-HP yağı tedavisi, p-zeytinyağı uygulamasına göre doku CAT seviyelerini arttırdı ve MDA seviyelerini düşürdü (p <0.05). Sonuçlar: Bu çalışmadan elde edilen sonuçlar, topikal p-HP yağı tedavisinin, içeriğindeki zeytinyağına göre antioksidan etkilere sahip olduğunu ve ikincil yara iyileşmesini hızlandırmak için kullanılabileceğini göstermiştir.

Anahtar Kelimeler:

Katalaz, hypericum perforatum yağı, malondialdehit, zeytinyağı, yara iyileşmesi

Antioxidant Characteristic of Prophylactic Hypericum Perforatum Oil Administration in a Rabbit Model of Palatal Mucosal Injury

Objective: This study was aimed to determine the antioxidant and soft tissue wound healing properties of prophylactic (p) application of topical Hypericum perforatum (HP) oil in rabbits. Materials and Methods: Thirty-six New Zealand albino rabbits were randomly classified as p-HP oil (test, n = 18) and p-olive oil (control, n = 18) groups. Each groups of rabbits were topically pretreated with either HP or olive oil before the surgery. Gingival biopsies were excised and analyzed for re-epithelialization (RE) and granulation tissue maturation (GTM) and clinical appearance were scored for erythema, epithelial confluence and general wound appearance on days 3, 7 and 14 after surgery. Levels of VEGF and FGF-2 were assessed immunohistochemically. Tissue catalase (CAT) and malondialdehyde (MDA) levels were measured using ELISA. Results: Higher epithelial confluence (on the 7th day) and general wound appearance scores (on the 7th and 14th days) were determined in p-HP oil than that of p-olive oil group (p<0.05). On the other hand RE scores did not differ between groups (p>0.05). The number of positively stained FGF-2 cells were higher in the test than that of control (p<0.05) but there were no significant differences between the groups for the number of positively stained VEGF vessels. p-HP oil treatment increased the tissue CAT levels and reduced the MDA levels compared to p-olive oil (p<0.05). Conclusions: The results obtained from this study indicated that topical p-HP oil treatment has antioxidant effects and may be used to accelerate the secondary wound healing compared to its base, olive oil.

Keywords:

catalase, hypericum perforatum oil, malondialdehyde, olive oil, wound healing,

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