Kitosan Bazlı Yüzeylerin Mezenşimal Kök Hücrelerin Nöronal ve Glial Yönde Farklılaşmalarına Etkileri

Doku mühendisliği, sinir sisteminin rejenerasyonu konusunda ümit vadeden yeni tekniklerden biridir.  Biyouyumlu ve biyobozunur doku iskelelerinin kök hücreler için taşıyıcı olarak kullanıldığı bu yöntemde, seçilen doku iskelesinin kimyasal ve fiziksel özellikleri, üzerine ekilen hücrelerin davranışlarını yönlendirerek tedavi sürecine katkıda bulunabilmektedir. Bu çalışmada biyouyumluluğu çok farklı dokular için gösterilmiş bir biyopolimer olan kitosan ile sülfat ve fosfat gruplarıyla fonksiyonelleştirilmiş türevlerinin sinir doku mühendisliği çalışmalarında kullanılma amacıyla sıçan kemik iliği kökenli mezenşimal kök hücrelerin (MKH) nöronal ve glial yönde farklılaşmalarına etkileri incelenmiştir. Hücre kültürü çalışmaları için sıçan kemik iliğinden MKH'ler izole edilmiş, osteojenik ve kondrojenik hücrelere farklılaşmaları sağlanarak hücrelerin farklılaşma potansiyelleri kalitatif yöntemlerle gösterilmiştir. Yüzey kimyasına hassas olduğu bilinen MKH’lerin kitosan, kitosan fosfat ve kitosan sülfat yüzeyler üzerinde tutunma ve proliferasyon özellikleri incelenmiştir. Kitosan ve kitosan fosfat yüzeylerde hücre tutunması düşükken, sülfat fonksiyonel grubunun eklenmesinin hücre tutunmasını anlamlı şekilde arttırdığı belirlenmiştir. MKH’ler kitosan sülfat yüzeyler üzerinde hem nöronal hem de glial yönde farklılaştırılabilmişlerdir. Bu çalışma, sinir doku rejenerasyonu ve doku mühendisliği amacıyla kitosan sülfat yüzey kimyasının kullanılabileceğini göstermektedir.

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Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi-Cover
  • ISSN: 2147-9607
  • Yayın Aralığı: Yılda 4 Sayı
  • Başlangıç: 2014
  • Yayıncı: Manisa Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü
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