Bazı Stres Faktörlerinin Aerobik Oksalat Bakterinin Kültüre Edilebilirliği ve Canlılığı Üzerine Etkileri

Çevresel faktörlerden sıcaklık, pH, besin, osmolarite ve antibiyotik stresinin Gram-negatif, çubuk şekilli aerobik oksalat bakterileri; Cupriavidus oxaliticus Ox1, Oxalicibacterium flavum TA17T, Xanthobacter sp. NS14 ve Cupriavidus necator NS02' nin kültüre edilebilirliği ve canlılığı üzerindeki etkisi belirlenmiştir. Hemen hemen 4 oksalat bakterisinin 30 °C koloni sayımları çok yüksek olmasına rağmen 40 ve 45 °C' de, en iyi büyüme gösterebilen suş TA17T' dir. pH değeri arttıkça bütün bakteri suşlarının koloni sayısının dereceli olarak kontrole göre (pH:7) azaldığı görülmüştür. pH 8'de en fazla koloni sayısı Ox1 suşunda gözlemlenmiştir. Besin stresi denemelerinde en iyi bakteriyal büyüme NS02 ve NS14 suşları için %0.6 potasyum oksalat (PO) ilaveli ortamda tespit edilmiştir. 4 organizmanın farklı konsantrasyonlardaki tuza (NaCI) karşı töleransları değişmekle beraber; NS14 suşunun %2 NaCl oranındaki ortamda koloni sayısı kontrole göre yüksektir. Organizmaların ampisilin antibiyotiğinin farklı konsantrasyonlarına karşı verdikleri cevap benzer olup; 2 µg/mL ve üzerindeki miktarlarında hiçbir suş üreme gösterememiştir. İndikatör olarak 2,3-5 trifenil tetrazolium klorit (TTC) kullanılarak yapılan canlılık belirleme testleri sonuçları ile koloni sayım sonuçları arasında bir korelasyon saptanamamıştır. Kullanılan oksalat bakterilerin uygulanan çevresel stres faktörlerine karşı verdikleri cevaplar farklılık göstermektedir. Çalışmada, özellikle yüksek pH ve sıcaklıkta büyüme gösterebilen oksalat bakterilerinin endüstriyel alanlarda kullanılabileceği öngörülmektedir.

Effects of Some Stress Factors on Culturability and Viability of Aerobic Oxalate Bacteria

The effects of environmental factors such as temperature, pH, nutrition, osmolarity and antibiotic stress for the determination of culturability and viability on gram negative rod shaped aerobic oxalate bacteria; Cupriviadus oxaliticus Ox1, Oxalicibacterium flavum TA17T, Xanthobacter sp. NS14 and Cupriavidus necator NS02 was studied. Colony counts at 30 °C for almost 4 oxalate bacteria were very high whereas optimum growth at 40 and 45 °C was seen for strain TA17T. Increasing the pH values were decreased the colony counts of all strains gradually, comparison with control (pH:7). The most colony count at pH 8 was observed with the strain Ox1. The nutrition stress experiments showed that the most bacterial growth was determined in the medium supplemented with 0.6% potassium oxalate (PO) for the strains NS02 and NS14. Tolerances of 4 organism at different concentrations of salt (NaCI) comparison with control were variable but colony counts of the strain NS14 was high at the 2% NaCI. Organisms reacts almost the same as the reaction against different ampicillin concentrations but at the 2 µg/mL and above no growth was observed for any strain. Comparison the colony count with viability test results that determined with the indicator 2,3-5 triphenly tetrazolium chloride (TTC); any correlations were not found. The used oxalate bacteria differentiate the reactions to applied environmental factors. It is foresighted that the oxalate bacteria capable growth especially at high pH and temperature can be used in industrial areas.

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