Elif YAVUZ, Güven ÇEVİK, Burcu ÇEVRELİ, Emel SERDAROĞLU KAŞIKÇI

579290

Effect of boric acid and quercetin combination on oxidative stress/ cognitive function in parkinson model

Parkinson's disease (PD) is a one of the important neurodegenerative disorders that affect dopaminergic neurons in the brain. Quercetin is one of the most potent antioxidants among all flavonoids, with direct radical scavenging activities and induction of antioxidative enzymes. Studies have shown that boric acid is essential for the activity of brain functions. We also used 3-month-old Wistar-albino male rats in our study, the experimental groups were divided into 8 groups (n=7) and Glutathione (GSH) and Malondialdehyde (MDA) levels were determined manually in brain tissue samples. Also, Total Antioxidant Status (TAS) was determined using a commercial kit. Locomotor Activity and Object Recognition tests were applied to evaluate the cognitive functions of the rats. GraphPad Prism 9 version was used for statistical analysis. Values were statistically analyzed by one-way ANOVA, differences between groups were determined using Uncorrected Fisher's LSD tests. p < 0.05 was considered statistically significant. In our study, we think that BA will positively affect the oxidant-antioxidant balance by preventing the pathogenesis of PD.
Anahtar Kelimeler:

Parkinson, , Rotenone, , Boric acid, , Quercetin, , Oxidative stress

Parkinson modelinde borik asit ve kuersetin kombinasyonu’nun oksidatif stres/ bilişsel fonksiyon üzerine etkisi

Parkinson hastalığı (PH), beyinde dopaminerjik nöronlara etki eden önemli bir nörodejeneratif bozukluktur. Kuersetin, doğrudan radikal temizleme aktiviteleri ve antioksidatif enzimlerin indüksiyonu ile tüm flavonoidler arasında en güçlü antioksidanlardan biridir. Yapılan çalışmalarda Borik asidin kalsiyum, vitamin D ve magnezyum ile etkileşime girerek organlar için yararlı etkilere sahip olduğu gösterilmiştir. Çalışmamızda 3 aylık Wistar-albino erkek sıçan kullandık, deney grupları 8 gruba (n=7) ayrıldı ve beyin doku örneklerinde Glutatyon (GSH) ve Malondialdehit (MDA) seviyeleri manuel olarak belirlendi. Aynı zamanda Total Antioksidan Durumu (TAS) ticari kit kullanılarak tayin edildi. Sıçanların bilişsel işlevlerini değerlendirmek için Lokomotor Aktivite ve Nesne Tanıma testleri uygulandı. İstatistiksel analizlerde GraphPad Prism 9 versiyonu kullanıldı. Değerler, tek yönlü ANOVA ile istatistiksel olarak analiz edildi, gruplar arasındaki farklılıklar Uncorrected Fisher's LSD testleri kullanılarak belirlendi. p < 0,05 istatistiksel olarak anlamlı kabul edildi. Buna göre çalışmamızın sonucunda Kuersetin ve Borik asidin antioksidan kapasite üzerine olumlu etkiler yaparak TAS seviyelerinde etkili olduğu gösterilmiştir. Çalışmamızda BA'nın PH patogenezini önleyerek oksidan-antioksidan dengeyi olumlu yönde etkileyeceği görüşündeyiz. Parkinson's disease (PD) is a one of the important neurodegenerative disorders that affect dopaminergic neurons in the brain. Quercetin is one of the most potent antioxidants among all flavonoids, with direct radical scavenging activities and induction of antioxidative enzymes. Studies have shown that boric acid is essential for the activity of brain functions. We also used 3-month-old Wistar-albino male rats in our study, the experimental groups were divided into 8 groups (n=7) and Glutathione (GSH) and Malondialdehyde (MDA) levels were determined manually in brain tissue samples. Also, Total Antioxidant Status (TAS) was determined using a commercial kit. Locomotor Activity and Object Recognition tests were applied to evaluate the cognitive functions of the rats. GraphPad Prism 9 version was used for statistical analysis. Values were statistically analyzed by one-way ANOVA, differences between groups were determined using Uncorrected Fisher's LSD tests. p < 0.05 was considered statistically significant. In our study, we think that BA will positively affect the oxidant-antioxidant balance by preventing the pathogenesis of PD.
Keywords:

Parkinson, , Rotenon, , Borik asit, , Kuersetin, , Oksidatif stres,

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Bor Dergisi-Cover
  • ISSN: 2149-9020
  • Yayın Aralığı: Yılda 4 Sayı
  • Başlangıç: 2016
  • Yayıncı: TENMAK Bor Araştırma Enstitüsü
Arşiv
Sayıdaki Diğer Makaleler

Effect of boric acid and quercetin combination on oxidative stress/ cognitive function in parkinson model

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