Assessment of the Antimicrobial Effectiveness of Hypericum Perforatum L. and Secondary Metabolites against Pathogenic Oral Bacteria

Amaç: Bu çalışmanın amacı, Hypericum perforatum L.’nin ve içindeki sekonder metabolitlerin, diş çürüğü ve periodontal hastalıklardan sorumlu mikroorganizmalar üzerine antimikrobiyal etkisini araştırarak, bu bitkinin ve sekonder metabolitlerinin insan gingival fibroblast hücreleri üzerine sitotoksisitelerini in vitro olarak incelemektir. Materyal ve Metot: Çalışmamızda Hypericum perforatum L. bitki ekstratı ve 6 ayrı sekonder metabolit (hyperforin, hypericin, hyperoside, quercetin, quercitrin, rutin) kullanıldı. Bu ürünlerin patojenik oral mikroorganizmalara [S. mutans, S. mitis, S. sanguinis, S. salivarius, S. sobrinus, L. acidophilus, P. gingivalis, A. actinomycetemcomitans, C. albicans] karşı antimikrobiyal aktivitesi disk difüzyon yöntemi ile incelenirken, MIC ve MBC değerleri de belirlendi. Tüm test materyallerinin oral dokular üzerine sitotoksisitesi, insan gingival fibroblast hücreleri kullanılarak MTT analizi ile değerlendirildi. Bulgular: Hypericum perforatum L. ekstratı ve sekonder metabolitlerinin çalışmamızda test edilen tüm oral patojenlere etkisi istatistiki olarak CHX’den düşük bulunmuştur. Bununla birlikte, periodontal hastalıkla ilişkili gram-negatif mikroorganizmalara ve C. albicans’a karşı CHX’ne yakın değerde inhibisyon zonu oluşturduğu görülen quercitrin’in, gingival fibroblast hücreleri üzerine CHX gibi şiddetli sitotoksisite göstermediği tespit edilmiştir. Sonuç: Bu çalışmada, bitki ekstratlarının saf bileşikler olmaması nedeniyle bu yönde yapılacak çalışmaların sadece bitkileri değil sekonder metabolitleri de incelemesi, yüksek antimikrobiyal etkinliğe sahip, düşük sitotoksik etkili yeni antimikrobiyal ve antifungal bitki ekstratları ve sekonder metabolitlerin bulunmasını mümkün kılacağı sonucuna varılmıştır.

Keywords:

Diş çürüğü, doğal antimikrobiyal ajanlar, hyperforin, hypericum peroratum L. hyperoside, quercitrin,

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