Bazı Domates Hatlarının Demir Noksanlığına Dayanıklılıklarının Belirlenmesi

Bu çalışmanın amacı, bazı domates hatlarının demir noksanlığına dayanıklılıklarının belirlenmesidir. Bu amaçla 445 g kuvars kumu ortamında, 12×3 (hat × demir dozu) şeklinde faktöriyel deneme yürütülmüştür. 12 farklı Tarbio (TB) domates hattına Fe-EDDHA formunda uygulanan demir dozları: Noksan (0,2 µM Fe), yeterli (45 µM Fe), yeterli (100 µM Fe)’dir. Denemede her muamele 3 tekerrürlü yapılmıştır. pH’sı 6,0’a ayarlı bitki besin çözeltisine yukarıda bildirilen demir konsantrasyonlarında Fe-EDDHA ilave edilmiştir. Bu şekilde farklı konsantrasyonlarda demir içeren besin çözeltisi deneme süresince günlük 50 mL olacak şekilde uygulanmıştır. Sera şartlarında deneme 50 gün sürdürülmüştür. Demir noksanlığı şartlarında birinci ana grupta TB-01, TB-10, TB-22 ve TB-65 nolu hatlar; ikinci ana grupta ise TB-14, TB-18, TB-28, TB-31, TB-35, TB-40, TB-90 ve TB-122 nolu hatlar yer almıştır. Birbirinden en uzak hatlar TB-01 ve TB-14 numaralı hatlar olup, bu hatlar karşılaştırıldığında TB-14 numaralı hattın demir noksalığı şartlarında kuru madde miktarı, klorofil-a, klorofil-b, toplam klorofil, karotenoid kapsamları ve yaprakta ferrik redüktaz aktivitesine ilişkin değerlerin TB-01 numaralı hatta göre daha yüksek olduğu tespit edilmiştir. Buna rağmen, demir noksanlığı şartlarında birbirine en uzak hatlar olmakla birlikte, TB-01 nolu hattın aktif demir kapsamının, kökte ferrik redüktaz aktivitesinin ve kök katyon değişim kapasitesinin TB-14 numaralı hatta göre daha yüksek olduğu görülmüştür. Demir noksanlığı şartlarında yetiştirilen domates hatlarından TB-18 ve TB-28 numaralı hatların birbirine en yakın hatlar oldukları da tespit edilmiştir.

Anahtar Kelimeler:

Domates hatları, Demir noksanlığı, Demir beslenme indeksleri, Toplam klorofil, Aktif demir, Ferrik redüktaz aktivitesi

Determining the Resistance of Some Tomato Lines to Iron Deficiency

The purpose of this study is to determine the resistance of some tomato lines to iron deficiency. For this purpose, a factorial trial was conducted as 12 × 3 (line × iron dose) in 445 g quartz sand media. Iron doses applied to 12 different Tarbio (TB) tomato lines in the form of Fe-EDDHA are: Deficient (0.2 µM Fe), sufficient (45 µM Fe), sufficient (100 µM Fe). In the experimet, each treatment was done in 3 replications. Fe EDDHA was added to the pH adjusted to 6.0 plant nutrient solution at the iron concentrations reported above. In this way, a nutrient solution containing iron in different concentrations was applied as 50 mL per day during the trial. The experiment continued for 50 days under greenhouse conditions. While lines TB-01, TB-10, TB-22 and TB-65 take place in the first main group under iron deficiency conditions; the second main group included lines TB-14, TB-18, TB-28, TB-31, TB-35, TB-40, TB-90 and TB-122. It has been determined that the lines farthest from each other are lines TB-01 and TB-14. When these lines were compared with each other, it was determined that the values of dry matter content, chlorophyll-a, chlorophyll-b, total chlorophyll, carotenoid contents and ferric reductase activity in the leaf under iron deficiency conditions of the line numbered TB-14 were higher than the line number TB-01. On the other hand, active iron content, root ferric reductase activity and root cation exchange capacity of tomato line numbered TB-01 were found to be higher than line TB-14. It was also determined that lines TB-18 and TB-28 of tomato lines grown under iron deficiency conditions were the closest lines to each other.

Keywords:

Tomato lines, Iron deficiency, Iron nutritional indexes, Total chlorophyll, Active iron, Ferric reductase activity,

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