Pedicularis comosa L. var. acmodonta Boiss. türünün antioksidan ve antikandidal etkilerinin belirlenmesi

Özet Bu çalışmanın amacı, Pedicularis comosa var. acmodonta (Orobanchaceae) türünün antioksidan ve antikandidal aktivitesini değerlendirmektir. Türkiye'de yetişen P. comosa var. acmodonta ekstreleri üzerinde bir spektrofotometrik test kullanılarak, bitki ekstrelerinin antioksidan aktivitesi, 1,1-difenilpiril hidrazil (DPPH·) içeren bir metanol solüsyonundaki mor rengin açılması ölçülerek değerlendirilmiştir. Ayrıca CLSI M27-A2 yöntemi kullanılarak yapılan antikandidal etki testlerinin sonuçları da bu çalışmada yer almaktadır. P. comosa'nın %70 etanol ekstresi en yüksek antioksidan aktiviteyi (0.143 mg/mL) göstermiştir. Beş Candida türü üzerinde yapılan antikandidal aktivite testi bulgularına göre P. comosa'nın %70 etanol ekstresinin C. utilis'i 31.25 µg/mL (MIC) dozunda inhibe ettiği belirlenmiştir.

Anahtar Kelimeler:

antikandidal, antioksidan, Pedicularis, Orobanchaceae.

Antioxidant and anticandidal effects of Pedicularis comosa L. var. acmodonta Boiss.

Abstract The aim of this study is to measure the antioxidant and anticandidal efficiency of Pedicularis comosa var. acmodonta (Orobanchaceae). Using a spectrophotometric test on extracts from P.comosa var. acmodonta grown in Turkey, the antioxidant efficiency of the plant extracts was estimated by measuring the violet coloration in a methanol solution containing 1,1-diphenylpyryl hydrazil (DPPH·). In addition, the results of the anticandidal effect tests using the CLSI M27-A2 method are also included in this study. The 70% ethanol extract of P. comosa showed the highest antioxidant activity (0.143 mg/mL). According to the anticandidal activity test findings on five Candida species, it was determined that 70% ethanol extract of P. comosa inhibited C. utilis at a dose of 31.25 µg/mL (MIC).

Keywords:

anticandidal, antioxidant, Pedicularis, Orobanchaceae.,

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