Erodium hoefftianum (Geraniaceae)’un Doku Kültürü Yöntemleriyle Üretiminin Araştırılması

Bu yeni yaklaşım, doğrudan organogenesis yoluyla Erodium hoefftianum'un üretim yöntemini belirlemek için tasarlanmıştır. Eksplant olarak Murashige ve Skoog (MS) besin ortamında çimlenen tohumlardan elde edilen yan meristemler kullanılmıştır. Daha sonra, ayrı ayrı 1.0/0.1 mg/L zeatin (ZEA)/indol-3- bütirik asit (IBA), 1.0/0.1 mg/L kinetin (KIN)/IBA, 1.0/0.1 mg/L N6-[2-isopentenil]adenin (2iP)/IBA, 1.0/0.1 mg/L 6- Benziladenin (6-BA)/IBA, 1.0/0.1 mg/L thidiazuron (TDZ)/IBA ve bitki büyüme düzenleyicisi olmadan desteklenen MS besin ortamı etkin sürgün çoğalması için araştırılmıştır. 1.0/0.1 mg/L 6-BA/IBA kombinasyonu sürgün uzunluğu hariç tüm durumlarda en uygun bitki büyüme düzenleyicileri olarak bulundu. Zeatin/IBA kombinasyonu 37.28 mm ile sürgün uzunluğu için en ideal olarak belirlendi. Köklenme kabiliyeti ayrıca IBA, indol-3-bütirik asit (IAA) ve 1-naftalen asetik asit (NAA) (0,5 mg/L) veya oksinsiz (kontrol) ile MS kullanılarak da incelenmiştir. Kontrol grubu, %100 köklenme yüzdesi ile tüm durumlarda en yüksek değerlere sahiptir. Turba: perlit (2:1) ortamlarına nakledilen köklü bitkicikler daha sonra iklim odası koşulları altında iklimlendirildi ve botanik bahçesi koşullarına transfer edildi.

Anahtar Kelimeler:

iklimlendirme, Erodium hoefftianum, ex situ, in vitro, mikroçoğaltma, PGR

Erodium hoefftianum (Geraniaceae)’un Doku Kültürü Yöntemleriyle Üretiminin Araştırılması

This current and novel approach was designed to occur the production method of Erodium hoefftianum via direct organogenesis. Lateral meristems obtained from seeds germinated in Murashige and Skoog (MS) basal medium were preferred as explants. Then, MS medium supplemented with individually 1.0/0.1 mg/L zeatin (ZEA)/indole-3- butyric acid (IBA), 1.0/0.1 mg/L kinetin (KIN)/IBA, 1.0/0.1 mg/L N6-[2-isopentenil]adenine (2iP)/IBA, 1.0/0.1 mg/L 6- Benzyladenine (6-BA)/IBA, 1.0/0.1 mg/L thidiazuron (TDZ)/IBA and without plant growth regulators (PGRs) were investigated for the effective shoot multiplication. 1.0/0.1 mg/L 6-BA/ IBA combination was found to be the most appropriate PGRs in all cases except shoot length. Zeatin/IBA combination was determined to be most ideal for shoot length with 37.28 mm. The rooting capability was also studied by using MS with IBA, indole-3- butyric acid (IAA), and 1-naphthaleneacetic acid, (NAA) (0.5 mg/L) or without auxin (control). The control group has the highest values in all cases with the rooting percentage at 100%. Well rooted and healty plantlets were transplanted into rich peat: perlite (2:1) substrates and subsequently acclimatized carefully under climate room conditions and transfer to the botanical garden conditions.

Keywords:

acclimatization, Erodium hoefftianum, ex situ, in vitro, micropropagation, PGR,

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