Nalan YILMAZ SARIÖZLÜ, Meral YILMAZ CANKILIÇ, Mehmet CANDAN

Bazı epifitik ve terrikol liken ekstraktlarının antifungal aktivitesinin araştırılması

İki terrikol Cetraria aculeata, Cladonia foliacea ve dört epifitik Parmelia sulcata, Pseudevernia furfuracea var. furfuracea, Ramalina farinacea ve Tornabea scutellifera likenin aseton, metanol ve kloroform ekstraktlarının Aspergillus fumigatus, Aspergillus parasiticus, Fusarium moniliforme, Fusarium solani ve Alternaria brassicicola fungal patojenlerine karşı antifungal aktivitesi incelenmiştir. Liken ekstraktlarının minimum inhibisyon konsantrasyonu MİK değerlerinin belirlenmesinde mikrobroth dilüsyon yöntemi kullanılmıştır. Liken ekstraktlarının MİK değerlerinin, test edilen funguslar için 156.25 ila 1250 µg/ml aralığında değiştiği belirlenmiştir. Genel olarak, liken ekstraktlarının tamamı güçlü antifungal aktivite göstermiştir. F. moniliforme ve A. brassicicola liken ekstraktlarına karşı en duyarlı fungal türler olarak belirlenirken, A. fumigatus en dirençli patojen fungus olarak bulunmuştur

Anahtar Kelimeler:

antifungal aktivite, liken ekstraktları, patojenik funguslar

Investigation of the antifungal activity of some epiphytic and terricolous lichen extracts

Antifungal activity of two terricolous Cetraria aculeata, Cladonia foliacea and four epiphytic Parmelia sulcata, Pseudevernia furfuracea var. furfuracea, Ramalina farinacea and Tornabea scutellifera lichens were investigated by using acetone, methanol and chloroform extracts against five fungal pathogens: Aspergillus fumigatus, Aspergillus parasiticus, Fusarium moniliforme, Fusarium solani and Alternaria brassicicola. The microbroth dilution method was used for minimal inhibitory concentration MIC values of the lichen extracts. The MIC values of the lichen extracts were determined ranging from 156.25 to1250 μg/ml for tested fungi. Generally, all of the lichen extracts showed the strong antifungal activity. F. moniliforme and A. brassicicola were the most sensitive fungal species against the lichen extracts, whereas A. fumigatus was the most tolerant pathogenic fungus

Keywords:

antifungal activity, lichen extracts, pathogenic fungi,

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