Aydın Şükrü BENGÜ, Mehmet Sait İZGİ, Mahire BAYRAMOGLU AKKOYUN, H. Turan AKKOYUN

Sıçan Böbrek Dokusunda Bakırın Neden Olduğu Oksidatif Stres ve DNA Hasarlarına Karşı Astaksantinin Etkisi

Sıçan böbrek dokusunda bakır (Cu+2) ile oluşturulan oksidatif stres ve DNA hasarlarına karşı astaksantin'in(AST) koruyucu özelliği araştırıldı. 28 adet yetişkin wistar albino cinsi sıçan dört gruba ayrıldı. Kontrol grubu:%0.9 NaCl intraperitoneal (i.p.) , Bakır grubu 3 mg kg-1(i.p.) Cu+2, Astaksantin grubu 100 mg kg-1 AST (oral),Bakır+Astaksantin grubu: 3 mg kg-1(i.p.) +100 mg kg-1 AST (oral) uygulandı. 3. günün sonunda sıçanlarınböbrek dokuları alındı. Böbrek homojenatlarında önemli antioksidan enzimlerden SOD, CAT, GPx aktivitelerive 8-hidroksi-2' -deoksiguanozin (8-OHdG) düzeyi değerlendirildi. SOD aktivitesinin bakır uygulanan gruptave bakır+AST uygulanan grupta kontrole oranla azaldığı(p˂0.05), gözlemlendi. GPx enzim aktivitesi isebakır(p˂0.001) uygulanan ve bakır+AST(p˂0.01) uygulanan gruplarda kontrole göre azaldığı belirlendi. CATaktivitesi, kontrol grubuna kıyasla bakır uygulanan grupta düşmenin(p

Effect of astaxanthin against oxidative stress and DNA damage caused by copper in rat kidney tissue

The protective properties of astaxanthin (AST) against copper (Cu+2) oxidative stress and DNA damage in rat kidney tissue were investigated. 28 adult wistar albino rats were divided into four groups. Control group: 0.9% NaCl intraperitoneal (ip), Copper group: 3 mg kg-1 (ip) Cu + 2, Astaxanthin group: 100 mg kg-1 AST (oral), Copper + Astaxanthin group: 3 mg kg-1 (ip) + 100 mg kg-1 AST (oral) was administered. At the end of day 3, kidney tissues of the rats were taken. SOD, CAT, GPx activities and 8-hydroxy-2 '-deoxyguanosine (8-OHdG) levels which are important antioxidant enzymes in kidney homogenates were evaluated. SOD activity was reduce observed in the copper treated group and copper + AST group compared to control (p

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[1] Kabak Y.B., Gülbahar M.Y. 2013. Sıçanlarda deneysel bakır zehirlenmesinde karaciğer ve böbrek dokularında apoptozisin belirlenmesi. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 60: 39-45.
[2] Turgut S., Ercan M., Turgut G., Zencir M., Genç O. 2000. Yüksek bakır ile çinkonun böbrek ve kalp üzerine etkileri. Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi,7 (3): 35-42.
[3] Akarsu T. 2013. Tokat ili Bölgesi Eser Elementleri (selenyum, çinko, bakır) Referans Aralıkları. Selçuk Üniversitesi, Sağlık Bilimleri Entitüsü, Yüksek Lisans Tezi, 21s, Konya.
[4] Güneş Ö.N. 2014. Hellp Sendromlu Hastalarda Serum Çinko Ve Bakır Düzeyleri. Atatürk Üniversitesi, Sağlık Bilimleri Enstitüsü, Yüksek Lisans Tezi, 41s, Erzurum.
[5] Cervantes-Cervantes M.P. Calderón-Salinas J.V., Albores A., Muñoz-Sánchez J.L. 2005. Copper Increases the Damage to DNA and Proteins Caused by Reactive Oxygen Species. Biological Trace Element Research, 103 (3): 229-248.
[6] Turkez H., Geyikoglu F., Yousef M.I. 2013. Beneficial Effect of Astaxanthin on 2, 3, 7, 8- tetrachlorodi benzo-p-dioxin-induced Liver Injury in Rats. Toxicology and Industrial Health, 29 (7): 591-599.
[7] Ekpe L., Inaku K., Ekpe V. 2018.Antioxidant Effects of Astaxanthin in Various Diseases—a Review. Journal of Molecular Pathophysiology, 7 (1): 1-6.
[8] Gao D., Li W. 2018. Research Progress of Astaxanthin on Contrast Agent Induced Acute Kidney Injury. Journal Cardiol and Cardiovasc Sciences, 2 (3): 6-9.
[9] Akca G., Eren H., Tumkaya L., Mercantepe T., Horsanali M.O., Deveci E., Yilmaz A. 2018. The Protective effect of Astaxanthin against Cisplatin-induced Nephrotoxicity in Rats. Biomedicine&Pharmacotherapy, 100: 575-582.
[10] Akkoyun H.T., Bengü A.Ş., Ulucan A., Akkoyun Bayramoglu M., Ekin S., Temel Y., Çiftci M. 2018. Effect of Astaxanthin on Rat Brains Against Oxidative Stress Induced By Cadmium:Biochemical. Histopathological Evaluation, 8 (4): 33-39.
[11] Zhou L., Gao M., Xiao Z., Zhang J., Li X., Wang A. 2015. ProtectiveEffect of Astaxanthin Against Multiple Organ Injury in a Rat Model of Sepsis. Journal of Surgical Research, 195 (2): 559-567.
[12] Irato P., Santon A., Ossi E., Albergoni V. 2001. Interactions between Metals in Rat Liver and Kidney: Localization of Metallothionein. The Histochemical Journal, 33 (2): 79-86.
[13] Ohno M., Darwish W.S., Miki W., Ikenaka Y., Ishizuka M. 2011. Astaxanthin can Alter CYP1A-dependent Activities via Two Different Mechanisms: Induction of Protein Expression and Inhibition of NADPH P450 Reductase Dependent Electron Transfer. Food and ChemicalToxicology, (6): 1285-1291.
[14] Sun Yi., Oberley L.W., Ying Li. 1988. A Simple Method for Clinical Assay of Superoxide Dismutase. Clinical Chemistry, 3413: 497-500.
[15] Donald E.P., Valentina W.N. 1967. Studies on the Quantitative Characterization of Erythrocyte Glutathione Peroxidase. Journal of Laboratory and Clinical Medicine, (70): 158-169.
[16] Aebi H. 1984. Catalase in Vitro Assay Methods. Methods Enzymology, 105: 121-126.
[17] Bradford M.M. 1976. AR rapid and Sensitive Method for the Quantation of Microgram Quantaties of Protein Utiliging the Principle of Protein-Dye. Binding Analytical Biochemistry,72: 248.
[18] Özyurt H., Çevik Ö., Özgen Z., Özden A.S., Çadırcı S., Elmas M.A., Şener G. 2014. Quercetin protects radiation-induced DNA damage and apoptosis in kidney and bladder tissues of rats. Free radical research, 48 (10): 1247-1255.
[19] Gonzalez-Hunt C.P., Wadhwa M., Sanders L.H. 2018. DNA Damage by Oxidative Stress: Measurement Strategies for Two Genomes. Current Opinion in Toxicology, 7: 87-94.
[20] Sanda A., Gal A., Pintea A., Bedecean I., Arion A., Baba A.I. 2008. Influence of Astaxanthin Administration on Hepatic Oxidative Stress Markers in Rats Injected with Methylnitrosurea. Bulletin UASVM, 65: 1.
[21] Gałażyn-Sidorczuk M., Brzóska M.M., Jurczuk M., Moniuszko-Jakoniuk J. 2009. Oxidative Damage to Proteins and DNA in Rats Exposed to Cadmium and/or Ethanol. ChemicoBiological Interactions, 180 (1): 31-38.
[22] Salmon T.B., Evert B.A., Song B., Doetsch P.W. 2004. Biological Consequences of Oxidative Stress-Induced DNA Damage in Saccharomycescerevisiae. Nucleic Acids Research, 32 (12): 3712-3723.
[23] Du W., Rani R., Sipple J., Schick J., Myers K.C., Mehta P., Pang Q. 2012. The FA Pathway Counteracts Oxidative Stress Through Selective Protection of Antioxidant Defense Gene Promoters. Blood, 119 (18): 4142-4151.
[24] Patlolla A.K., Barnes C., Yedjou C., Velma V.R., Tchounwou P.B. 2009. Oxidative Stress, DNA Damage, and Antioxidant Enzyme Activity Induced by Hexavalent Chromium in Sprague‐DawleyRats. Environmental Toxicology: An International Journal, 24 (1): 66-73.
[25] Akkoyun H.T., Bengu A.S., Ulucan A , Bayramoglu-Akkoyun M., Arihan O. 2018. Protective Effect Of Ellagic Acid Against Carbon Tetrachloride (CCl4) Induced Oxidative Brain Injury In Rats. Fresenius Environmental Bulletin, 27 (5): 3148-3155.
[26] Otitoju O., Onwurah I.N., Otitoju G.T., Ugwu C.E. 2008. Oxidative Stress and Superoxide Dismutase Activity in Brain of Rats Fed with Diet Containing Permethrin. Biokemistri, 20 (2): 93-98.
[27] Elguindy N.M., Yacout G.A., El Azab E.F. 2018. Amelioration of DNA-Induced Oxidative Stress in Rat Kidney and Brain by the Essentialoil of Elettaria Cardamomum. Beni-Suef University Journal of Basic and Applied Sciences, 7 (3): 299-305.
[28] Augusti P.R., Conterato G.M.M., Somacal S., Sobieski R., Spohr P.R., Torres J.V., Emanuelli T. 2008. Effect of Astaxanthin on Kidney Function Impairment and Oxidative Stress Induced by Mercuric Chloride in Rats. Food and Chemical Toxicology, 46 (1): 212-219.
[29] Jing C., Wenhua L., Nana L., Yaren Y., Zheng D. 2015. Effect of Astaxanthin Intervention on Contrast-Induced Acute Kidney Injury in Experimental Rats. Chinese Journal of Nephrology, 31 (8): 604-609.
[30] Akkoyun H.T. 2012. Fötal Dönemde Nikotine Maruz Kalan Sıçanlarda Oluşan Böbrek Hasarının Engellenmesinde Ellagic Asitin Koruyucu Etkilerinin İncelenmesi. Atatürk Üniversitesi Sağlık Bilimleri Enstitüsü, Doktora Tezi, 66s, Erzurum.
[31] Gao D., Wang H., Xu Y., Zheng D., Zhang Q., Li W. 2019. Protective Effect of Astaxanthin Against Contrast-Induced Acute Kidney Injury via SIRT1-p53 Pathway in Rats. International Urology and Nephrology, 51 (2): 351-358.
[32] Wang X., Zhao H., Shao Y., Wang P., Wei Y., Zhang W., Zhang Z. 2014. Nephroprotective Effect of Astaxanthin Against Trivalent Inorganic Arsenic-Induced Renal Injury in Wistar Rrats. Nutrition Research and Practice, 8 (1): 46-53.
[33] Kasai H., Nishimura S. 1993. Formation of 8-hydroxyguanine by Oxidative DNA Damage, its Repairandits Mutagenic Effects. In de Obe,G. (ed.) Advances in Mutagenesis Research. Springer-Verlag, Berlin, Germany, 4: 31-45.
[34] Umemura T., Sai K., Takagi A., Hasegawa R., Kurokawa Y. 1990. Formation of 8 hydroxydeoxyguaosine (8-OH-dG) in Rat Kidney DNA After Intraperitoneal Administration of Ferric Nitrilotriacetate (Fe-NTA). Carcinogenesis, 11: 345-347.
[35] Cheng K.C., Cahill D.S., Kasai H., Nishimura S., Loeb L.A. 1992. 8-Hydroxyguanine, an Abundant form of Oxidative DNA Damage, Causes G→T and A→C Substitutions. Journal of Biological Chemistry, 267: 166-172.