Ayşe BALDEMİR KILIÇ, Cevahir ALTINKAYNAK, Nalan ÖZDEMİR, Vedat YILMAZ, İsmail ÖÇSOY

Kudret Narı (Momordica charantia Descourt.) Meyvesinden Saflaştırılan Peroksidaz Enzimi Kullanılarak Hibrit Nano Çiçekler Sentezlenmesi ve Direct Blue 1 Gideriminde Kullanılabilirlikleri

Farklı bitkisel kaynaklardan saflaştırılan peroksidaz enzimleri verimli bir şekilde endüstriyel atıklarda yer alanboyar maddelerin gideriminde kullanılmaktadır. Ucuz ve kolay ulaşılabilir bitkisel bir kaynak olan kudret narı(Momordica charantia) meyvesi önemli bir peroksidaz kaynağıdır. Bu nedenle çalışmada kudret narı meyvesininham ve olgun halinden amonyum sülfat çöktürme yöntemi ile saflaştırılan peroksidaz enzimi kullanılarak çiçekşekilli hibrit nano yapı elde edilmesi ve enzimatik aktivitelerinin karşılaştırmalı olarak ölçülerek direct blue 1 boyagideriminde kullanılabilirlikleri hedeflenmiştir. Elde edilen verilere göre ham kudret narı meyvesinden yapılan%50 oranında protein çöktürmesi sonucu toplam protein miktarı 0,485 mg/mL bulunurken peroksidaz aktivitesi2360,9 EU/mg olarak tespit edilmiştir. Bunun yanında olgun kudret narı meyvesinden yapılan %60 oranındaprotein çöktürmesi sonucu ise toplam protein miktarı 0,232 mg/mL iken serbest peroksidaz aktivitesi 7719,30EU/mg olarak tespit edilmiştir. Farklı büyüme safhalarında yer alan meyvelerden optimum koşullardasaflaştırılmış peroksidaz enzimleri enzim-inorganik hibrit nano çiçek sentez yöntemi ile immobilize edildiğindeserbest formlarına göre daha yüksek enzimatik aktivite sergilemişlerdir. En yüksek peroksidaz aktivitesi olgunmeyvede ve hibrit nano çiçek formunda (19661,6 EU/mg) görülmüştür. Ayrıca çalışmada tekstil endüstrisindeyaygın olarak kullanılan Direct Blue 1 boyasının giderimi için hibrit nano çiçeklerin serbest peroksidaz enzimleriile karşılaştırmalı olarak kullanılabilirlikleri araştırılmıştır. Özellikle ham meyve peroksidazı kullanılaraksentezlenen hibrit nano çiçek formunun daha fazla boya giderimi yaptığı tespit edilmiştir.

Synthesis of Hybrid Nanoflowers Using The Purified Bitter Gourd (Momordica charantia Descourt.) Peroxidase and its Usability Of Direct Blue 1 Decolorization

Peroxidase enzymes are purified from different plant sources are used efficiently for the removal of dyes in industrial wastes. The fruit of bitter gourd (Momordica charantia), an inexpensive and easily accessible vegetable, is an important source of peroxidase. For this reason, the aim of this study was to investigate hybrid nanoflower by using purified peroxidase enzyme from green and ripe form of bitter gourd via ammonium sulphate precipitation method, and to compare their enzymatic activities by direct blue 1 dye removal. According to the data, total protein content was found to be 0.485 mg/mL and peroxidase activity was found to be 2360.9 EU/mg as a result of 50% protein precipitation made from green bitter gourd. However; total protein amount was 0.232 mg/mL and freeperoxidase activity was determined as 7719.30 EU/mg as a result of 60% protein precipitation made from ripe bitter gourd. Peroxidase enzymes which were purified from bitter gourd in different growth stages under optimum conditions showed higher enzymatic activity compared to free forms when immobilized via enzyme-inorganic hybrid nanoflower synthesis method. The highest peroxidase activity was seen in mature fruit and hybrid nanoflower form (19661, 6 EU/mg). In addition, the usability of hybrid nanoflowers was investigated compared to the free purified peroxidase for removal of Direct Blue 1 dye widely used in textile industry. It was determined that hybrid nanoflower form synthesized especially by using ripe bitter gourd peroxidase had more dye removal.

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