Trakya Bölgesi'nde hasat edilmiş kanola (Brassica napus L.) tohumlarında tohum kökenli fungal etmenlerin tespiti

Bu çalışmada 2013 yılında Trakya Bölgesi'nde bulunan Edirne, Kırklareli ve Tekirdağ illerinden toplanan kanola tohumu örneklerinde fungal etmenlerin tespiti ve patojenisitelerinin belirlenmesi amaçlanmıştır. Çalışmada ayrıca yüksek derecede patojen bulunan fungus türlerinin kültürel ve morfolojik özellikleri tanımlanmıştır. Çalışma sonucunda Trakya Bölgesi'ndeki illere ait tohum örneklerinde yapılan incelemelerde tohumların en yüksek oranda Alternaria cinsi içindeki alternata türleri grubundan A. alternata ile bulaşık olduğu, bunu infectoria türleri grubundan A. ethzedia ve A. infectoria'nın izlediği belirlenmiştir. Tohum örneklerinde tespit edilen diğer funguslar ise Arthrinium arundinis, Cladosporium sp., Curvularia sp., Fusarium sp., ve Phomopsis sp. olarak tanımlanmış olup, bunlar arasında en yaygın türün A. arundinis olduğu görülmüştür. Fungal etmenlerle yapılan patojenisite testleri sonucunda, A. alternata izolatları %52.2- %76.0, A. ethzedia izolatları %31.5-%82, A. infectoria izolatları %24.7-%70.5 ve A. arundinis izolatları %56.20-%69.20 arasında değişen oranlarda hastalık şiddeti oluşturmuşlardır.

Anahtar Kelimeler:

Kanola (Brasicca napus L.), tohum kökenli funguslar, patojenisite

Determination of seed-borne fungal pathogens on harvested canola (Brassica napus L.) seeds from Thrace Region

The aim of this study was to determine fungal pathogens on seed samples of canola collected in 2013 from the fields located on Edirne, Tekirdağ, Kırklareli provinces of Thrace region, and their pathogenicity. Additionally, the cultural and morphological characteristics of fungal species, which were highly pathogenic, were determined. As a result of the study, it was found that seed samples collected from Thrace region of Turkey were contaminated with A. alternata in the alternata species groups of Alternaria genus at the highest rate, A. ethzedia and A. infectoria in the infectoria species groups of the same genus followed it. Other species detected in canola seed samples were Arthrinium arundinis, Cladosporium sp., Curvularia sp., Fusarium sp., Phomopsis sp. and the most common species of these was A. arundinis. In the result of pathogenicity tests, A. alternata isolates caused disease severity between 52.2-76% on seedling; the isolates of A. ethzedia had virulence capacity ranged from 31.5 to 82%. This range was between 24.7-70.5% for A. infectoria and between 56.20-69.20% for A. arundinis.

Keywords:

Canola (Brassica napus L.), seed-borne fungi, pathogenicity,

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