Onur DURA, Adnan TÜLEK, İbrahim SÖNMEZ, Fatma Dolunay ERDOĞUŞ, Ayşe YEŞİLAYER, İlker KEPENEKCİ

Lantana camara L. (Lamiales: Verbenaceae)'nın sulu ekstraktı kullanılarak hazırlanan gümüş nanopartikül (AgNPs) uygulamalarının Buğday gal nematodu [Anguina tritici Thorne, 1949 (Nematoda: Anguinidae)]’na etkileri

Buğdayı enfekte eden Buğday gal nematodu [Anguina tritici Thorne, 1949 (Nematoda: Anguinidae)] buğday tarımı yapılan yerlerde en yaygın olan ve ekonomik zarara neden olan bitki paraziti nematod (BPN) türlerinden birisidir. A. tritici tohum kaynaklı bir nematod türü olup kuru koşullarda depolanmış tohumlarda uzun yıllar canlılığını sürdürebilmektedir. BPN’lere karşı mücadelede bazı bitki ve bitkisel ürünler alternatif nematod yönetimi bakımından kanıtlanmış etkiye sahiptir. Bu çalışmada in vitro koşullarda (Petri kapları deneyi) A. tritici’ye karşı çevre dostu potansiyel nematisit olarak Lantana camara L. (Lamiales: Verbenaceae)’nın sulu ekstraktı kullanılarak hazırlanan gümüş nanopartikülleri (AgNPs)’nin etkisi değerlendirilmiştir. L. camara’nın sulu ekstraktı kullanılarak gümüş nanopartikülleri 3 farklı konsantrasyonda [42 ppm (0.25 mM), 84 ppm (0.5 mM) ve 168 ppm (1 mM)] kullanılmıştır. Deneme 4 karakter [42 ppm, 84 ppm, 168 ppm uygulamalar ve pozitif kontrol K (+)] ve 4 tekerrürlü olarak kurulmuş, çalışmalar Atatürk Bahçe Kültürleri Merkez Araştırma Enstitüsü (Yalova, Türkiye)’de yürütülmüştür. A. tritici popülasyonu 4 tekerrürlü olacak şekilde steril petrilere transfer edilmiş ve Petri kapları 28±2 °C’de tutulmuştur. A. tritici bireyleri ince iğne ile dokunulduğunda hareket etmediği takdirde ölü olarak kabul edilmiştir. Bütün konsantrasyonlar farklı oranlarda nematisidal etki göstermiştir. L. camara’nın sulu ekstraktı kullanılarak hazırlanan gümüş nanopartiküllerinin etkisi 168 ppm (1 mM) konsantrasyonda 72 saat sürede A. tritici bireylerinde %97 ölüme sebep olmuştur.

Anahtar Kelimeler:

Lantana camara, nematod, gümüş nanopartikülleri, Anguina tritici

Effects of silver nanoparticle (AgNPs) applications prepared using Lantana camara L. (Lamiales: Verbenaceae)'s aqueous extract on Wheat gal nematode [Anguina tritici Thorne, 1949 (Nematoda: Anguinidae)]

Wheat seed gall nematode [Anguina tritici Thorne, 1949 (Nematoda: Anguinidae)] is one of the plant-parasitic nematodes (PPNs) causing economic yield losses in wheat-growing areas. A. tritici is a seed born nematode and can survive in stored seed for many years under dry conditions. Some plants and botanical products were proved to be effective to PPNs control in terms of alternative nematode management tactics. In this study, silver nanoparticles prepared using Lantana camara L. (Lamiales: Verbenaceae) aqueous extract (AgNPs) as an eco-friendly control of nematodes were evaluated against A. tritici in in vitro conditions (Petri dishes experiment). Silver nanoparticles using L. camara aqueous extract were used in 3 different concentrations [42 ppm (0.25 mM), 84 ppm (0.5 mM) and 168 ppm (1 mM)] were used. The experiments were conducted as randomized block parcel design with four replicates and four characters [42 ppm, 84 ppm, 168 ppm applications, and positive control C (+)] in Atatürk Central Horticultural Research Institute (Yalova, Turkey). A. tritici individuals were transferred into sterilized Petri dishes in four replicates and kept at 28±2 °C. A. tritici individuals were considered dead if there were no motion when touched with a fine needle. All the concentrations showed different of antinematodal activity. Silver nanoparticles at concentrations of 168 ppm (1 mM) prepared using L. camara aqueous extract caused 97% A. tritici mortality in 72 hours.

Keywords:

nematode, silver nanoparticles, Lantana camara, Anguina tritici,

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