2-(karbazol-3’-il)-5-formilpiridin: Floresans Biyotiyol Sensör Özelliğinin İncelenmesi

Bu çalışmada 2-(karbazol-3’-il)-5-formilpiridin molekülü floresans prob olarak biyotiyol (sistein, homosistein ve glutatyon) moleküllerinin tayin ve tespitinde kullanıldı. Probun bu biyotiyollere karşı duyarlılık ve seçiciliği UV-vis ve PL spektrometreleri ile incelendi. Prob, 1:9 DMSO:HEPES Tampon çözeltisi (0.1 M, pH:7.4) içerisinde biyotiyoller arasından sisteine karşı 60 eşdeğerde emisyon artışı ile seçicilik gösterdi. Ayrıca prob ile biyotiyoller arasında oluşan yeni yapı 1H NMR ve MS spektrometreleri ile de takip edilerek teyit edildi.

Anahtar Kelimeler:

Aldehit Grup, Biyotiyol, Fiziksel Özellikler, Karbazol, Sensör

2-(carbazole-3'-yl)-5-formylpyridine: Investigation of the Fluorescence Biothiol Sensor Property

In this study, 2-(carbazole-3'-yl)-5-formylpyridine molecule was used as a fluorescence probe for the determination and detection of biothiol (cysteine, homocysteine and glutathione) molecules. The sensitivity and selectivity of the probe to these biothiols were examined with UV-vis and PL spectrometers. The probe showed selectivity among biothiols in 1:9 DMSO:HEPES solution (0.1 M, pH:7.4) with an emission increase of 60 equivalents against cysteine. In addition, the new structure formed between the probe and the biothiols was confirmed by monitoring with 1H NMR and MS spectrometers.

Keywords:

Aldehyde Group, Biothiol, Physical Properties, Carbazole, Sensor,

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