With the industrial revolution and the continuation of the production of motor vehicles in the historical process, motor vehicles became a part of daily life. This rapid change in transportation has increased the social and economic mobility in the cities and caused the city population to increase and increase rapidly. As cities continue to grow rapidly with the population, the journey in cities has increased their distance and duration. These developments in transportation have helped populate cities with more than millions. Therefore, the demand for transportation has increased in high-population cities. The high number of demands in urban transportation and mobility in transportation paved the way for intensive vehicle traffic. Traffic obstructions in the city affect daily life badly. Solutions were tried to be produced for transportation problems. Every solution brought was slow and subsequently resulted in increased demand.Transportation problems caused by fast and unplanned urbanization negatively affect the quality of life in cities. The bicycle, which is actively used in various countries of the world, is considered as a means of transportation on its own. Unfortunately, the use of bicycles could not be integrated into urban transport, since the importance of cycling in our country is not given sufficient importance and the required infrastructure is not created. Especially in our big cities, transportation planning based on motor vehicles has limited the transportation of pedestrians and bicycles and it has become impossible to reach from one point to another by pedestrian or bicycle in big cities. However, bicycle, which is a comfortable and convenient means of transportation for short trips, is the vehicle that should be preferred in terms of environment, health and traffic. Bicycle is one of the most environmentally friendly modes of transportation. Compared to motor vehicles, bicycle-based manpower is a means of transportation that does not contain any material that can cause noise and air pollution. Preferring to use bicycles in business, school, or shopping trips in daily life provides important benefits not only for the environment but also for human health. The use of bicycles, which allows people to do physical activity, causes problems such as cardiovascular diseases and obesity to decrease. Considering all these, the importance and necessity of bicycle transportation are clearly seen.It is desired to increase the use of bicycles as a solution to the traffic problem, which is one of the biggest problems of today. To make the bicycle roads more accessible and safe, many bicycle road applications are made in our country and the world. Although the use of bicycles in our country is not very common in large cities, it is more common in medium and small-scale cities. Security can be shown as the reason for this. As well as the safety of the bicycle roads, the level of comfort these roads provide to the rider is also very important. Bicycle routes are needed to use bicycles in a big city like Istanbul. Every bike path has features that it should have. It is very important to apply and maintain these rules, which must be followed when designing bicycle paths.In this study, the cycle path route between Bakırköy and Yenikapı has been investigated. A literature review was conducted in the study. With the literature review, information was obtained about the design of bicycle paths by considering the regulations and standards. As a result, the design criteria of the cycle path were determined according to the standards in the regulation. Also, it has been determined which parameters the bicycle users pay attention to in choosing the route. The examinations were based on standards and regulations. The inspections made along the route are shown in the study with photos. The research detailed with photographs was evaluated. Solutions and suggestions regarding the factors affecting the use of bicycles are presented.