Pınar ÇIRAGİL, Ergül Belge KURUTAŞ, Ömer Faruk KÖKOĞLU, Murat ARAL

Oxidative Stress in Patients with Chronic Hepatitis B and C

Objective: Hepatitis B and hepatitis C virus infections are extensively seen throughout the world. Disturbances in the antioxidant system and oxidative stress may play a role in the pathogenesis of chronic liver diseases. The aim of this study was to evaluate the oxidant/antioxidant status in the serum of patients with chronic hepatitis B (CHB) and C (CHC). Material and Methods: Twenty patients with CHB, 20 with CHC and 30 healthy volunteers were included in this study. Catalase (CAT), superoxide dismutase (SOD) activities and malondialdehyde (MDA) levels were measured in all patients and control group specthrophotometrically. Results: While CAT and SOD activities were significantly lower in patients with CHC, these enzymes were significantly higher in patients with CHB compared to controls. However, MDA levels were increased in each patient group as compared to the control group. Also, antioxidant enzyme activities were found to be significantly lower and MDA levels were higher in patients with CHC, compared to patients with CHB. Conclusion: We considered that insufficiency of antioxidant barrier may cause oxidative stress in patients with CHB and CHC, so antioxidant treatment should be useful for these patients.

Keywords:

HBV, HCV, antioxidant enzymes, malondialdehyde,

PDF

___

Robinson WS. Hepatitis B virus and Hepatitis D virus. In: Mandel GL, Bennett JE, Dolin R, editors. Principles and Practice of Infec- tious Diseases. 5th ed. New York: Churchill Livingston; 2000. pp. 1652-84.
Thomas DL, Lemon SM. Hepatitis C. In: Mandel GL, Bennett JE, Dolin R, editors. Principles and Practice of Infectious Diseases. 5th ed. New York: Churchill Livingston; 2000. pp. 1736-59.
Sies H. Strategies of antioxidant defences. Eur J Biochem 1993;215:213-9. [CrossRef]
Rosser BG, Gores GJ. Liver cell necrosis: cellular mechanisms and clinical implications. Special reports and reviews. Gastroenterol- ogy 1995;108:252-75. [CrossRef]
Broide E, Klinowski E, Koukoulis G, Hadzic N, Portmann B, Baker A, et al. Superoxide dismutase activity in children with chronic liver diseases. J Hepathol 2000;32:188-92. [CrossRef]
Irshad M, Chaudhuri PS, Joshi YK. Superoxide dismutase and to- tal antioxidant levels in various forms of liver diseases. Hepatol- ogy Research 2002;23:178-84. [CrossRef]
Chrobot AM, Szaflarska-Szczepanik A, Drewa G. Antioxidant de- fence in children with chronic viral hepatitis B and C. Med Sci Monit 2000;6:713-8.
Demirdag K, Yilmaz S, Ozdarendeli A, Ozden M, Kalkan A, Kilic SS. Levels of plasma malondialdehyde and erythrocyte antioxi- dant enzyme activities in patients with chronic hepatitis B. Hepa- togastroenterology 2003;50:766-70. [CrossRef]
Boya P, de la Peña A, Beloqui O, Larrea E, Conchillo M, Castelruiz Y, et al. Antioxidant status and glutathione metabolism in periph- eral blood mononuclear cells from patients with chronic hepatitis C. J Hepatol 1999;31:808-14. [CrossRef]
De Maria N, Colantoni A, Fagiuoli S, Liu G-J, Rogers BK, Fari- nati F, et al. Association between reactive oxygen species and diseaseactivity in chronic hepatitis C. Free Radic Biol Med 1996;21:291-5. [CrossRef]
Paradis V, Mathurin P, Kollinger M, Imbert-Bismut F, Charlotte F,Piton A, et al. In situ detection of lipid peroxidation in chronic hepatitis C:correlation with pathological features. J Clin Pathol 1997;50:401-6. [CrossRef]
Romero MJ, Bosch-Morell F, Romero B, Rodrigo JM, Sera MA, Romero FJ. Serum Malondialdehyde: Possible use for the clinical management of chronic hepatitis C patients. Free Radic Biol Med 1998;25:993-7. [CrossRef]
Fridovich I. Superoxide radical: an endogenous toxicant. Annu Rev Pharmacol Toxicol 1983;23:239-57. [CrossRef]
Beutler E. Red: Cell Metabolism. Grune and Stratton Company, New York 1975;261-5. [CrossRef]
Ohkawa H, Ohishi N, Tagi K. Assay for lipid peroxides in animal tis- sues by thiobarbituric acid reaction. Anal Biochem 1979;95:351-8. [CrossRef]
Lowry O, Rosebrough NJ, Farr AL, Randall RJ. Protein mea- surement with the folin phenol reagent. J Biochem Chem 1951;193:265-75.
Nagoev BS, Abidov MT, Ivanova MR. LPO and free radical oxida- tion parameters in patients with acute viral hepatitis. Bull Exp Biol Med 2002;134:557-8. [CrossRef]