Farklı çimlenme sürelerinde adzuki fasulyesi (Vigna angularis) filizlerinin antioksidan aktivite ve biyokimyasal parametreleri üzerine çözücü konsantrasyonlarının etkisi

Bu çalışmada, farklı çimlenme sürelerinde (0, 24, 48, 72, 96 ve 120 saat) elde edilen adzuki fasulyesi (Vigna angularis) filizlerinin, su ve çeşitli organik çözücülerde (aseton, n-heksan ve etanol) ve farklı konsantrasyonlarda (% 50, 70 ve 90) ekstrakte edilerek DPPH radikal süpürme gücü, metal-iyon şelatlama aktivitesi, gama aminobütirik asit ve fitik asit düzeyleri, toplam fenolik madde içeriği belirlenmiştir. Ekstraksiyon verimi %11.47 (H3) ila %28.55 (E1) arasında değişmiştir. En yüksek DPPH radikal süpürme kapasitesi, 120 saat boyunca E2 konsantrasyonunda (2.978 µmol/g DW) belirlendi (P<0.05). E3 (1.744 mg EDTA eşdeğeri/100 g) ve A3 (1.145 mg EDTA eşdeğeri/100 g), 48 saatlik çimlenmeden sonra en yüksek metal şelatlama aktivitesini gösterdi. Bu aktivite, çimlenme periyodunda 48 saatten 120 saate düştü (P<0.05). Farklı çözücü konsantrasyonları karşılaştırıldığında, 0, 24 ve 48 saatlik analizlerde gama aminobütirik asit ve fitik asit içeriklerinde önemli bir değişiklik (P>0.05) olmadı. En yüksek gama aminobütirik asit içeriği, 120. saatte A1 (67.29 mg/100 g DW) ve H1 (69.17 mg/100 g DW) konsantrasyonlarında tespit edildi (P<0.05). 48 saatte tüm solvent konsantrasyonlarında toplam fenol içeriğinde önemli bir değişiklik bulunmadı (P>0.05). 120 saat sonunda, toplam fenolik bileşenler su ekstraksiyonunda en düşük seviyelerde ve E2'de en yüksek konsantrasyonlarda belirlendi (P<0.05). Bu sonuçlar, çimlenme süresinin artmasıyla birlikte biyoaktif bileşenlerin aktivitelerindeki artışa, anti-besinsel faktörlerin azalmasına, su ve sulu organik çözücülerdeki konsantrasyona bağlı olarak adzuki fasulyesi tohumlarının belirlenen parametrelerde daha etkili olabileceğini göstermiştir.

Anahtar Kelimeler:

Çimlenme süresi, adzuki fasulyesi, çözücü konsantrasyonları, filizlenme, antioksidan aktivite, fitik asit, GABA

Effect of solvent concentrations on antioxidant activity and biochemical parameters of adzuki bean (Vigna angularis) sprouts at different germination times

In this study were determined DPPH radical scavenging power, metal-ion chelating activity, gamma aminobutyric acid and phytic acid levels, total phenolic substance content, extraction yield in water and various organic solvents (acetone, n-hexane and ethanol), concentrations (50, 70 and 90%) and germination times (0, 24, 48, 72, 96 and 120 h) of adzuki bean (Vigna angularis) sprouts. The extraction yield ranged from 11.47% (H3) to 28.55% (E1). The highest DPPH radical scavenging capacity was determined at E2 concentration (2.978 µmol/g DW) for 120 h (P<0.05). E3 (1.744 mg EDTA equivalent/100 g) and A3 (1.145 mg EDTA equivalent/100 g) showed the highest metal chelating activity after 48h of germination. This activity decreased in the germination period from 48 h to 120 h (P<0.05). When different solvent concentrations were compared no significant change (P>0.05) in gamma aminobutyric acid and phytic acid contents at 0, 24 and 48 h analyses. The highest gamma aminobutyric acid content was detected to A1 (67.29 mg/100 g DW) and H1 (69.17 mg/100 g DW) concentrations at 120 h (P<0.05). No significant changes were found in total phenol content in all solvent concentrations in 48 h (P>0.05). At the end of 120 h, total phenolic components were determined in the lowest levels in W and the highest concentrations in E2 (P<0.05). These results showed that adzuki bean seeds may be more effective in these parameters, depending on the increase in the activities of bioactive components and the decrease in anti-nutritional factors, and the concentration in water and aqueous organic solvents with the increase of germination time.

Keywords:

Germination time, adzuki bean, solvent concentrations, sprout, antioxidant activity, phytic acid, GABA,

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