Siyah ve Yeşil Çay İle Üretilen Kombucha Çaylarının Antimikrobiyal ve Antioksidatif Özellikleri
Bu çalışmada iki farklı çay ile üretilen Kambucha çaylarının fermantasyon süresi sonundaki antikrobiyal ve antioksidatifözelliklerinin belirlenmesi amaçlanmıştır.18 günlük fermantasyon süresi sonunda siyah çay ile üretilen çayların Serbest radikalgiderme aktivitesinin (% 62.4±0.8) ve Toplam fenolik madde miktarlarının (7.8±1.3 GAE, mM) yeşil çay ile üretilenlere kıyasla daha yüksek olduğu belirlenmiştir. 8 farklı gıda kaynaklı patojen bakteri üzerinde yapılan antimikrobiyal aktivite testi sonucunda en fazla etkinin yine siyah çay ile üretilen Kambucha çaylarında Staphylococcus aureus (24 mm zon çapı) üzerinde olduğu, bunu Escherichia coli’nin (20 mm zon çapı) izlediği tespit edildi. Aynı bakteriler üzerinde siyah çay kullanılarak üretilen çeşidin MIC değerleri ise sırasıyla 24 ve 48 μg/mL olarak belirlendi.
Antimicrobial and Antioxidative Properties of Kombucha Teas Produced with Black and Green Tea
In this study, it is aimed to determine the anticrobial and antioxidative properties of the Kambucha teas produced by two different teas at the end of the fermentation period. At the end of the fermentation period of 18 days, it was determined that the free radical removal activity (% 62.4 ± 0.8) and total phenolic content (7.8 ± 1.3 GAE, mM) of teas produced with black tea were higher than those produced with green tea. The antimicrobial activity test on 8 different foodborne pathogenic bacteria was found to have the highest effect on the Staphylococcus aureus (24 mm zone diameter) in the Kambucha teas produced by black tea, followed by the Escherichia coli (20 mm zone diameter). MIC values of the same bacteria were determined as 24 and 48 μg / mL, respectively.
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- Anonim, 2018. Eucast, European Commitee on
Antimicrobial Susceptibilty Testing,
http://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files
/Breakpoint_tables/v_8.0_Breakpoint_Tables.pdf
- Battikh H., Bakhrouf A. and Ammar E. 2012. Antimicrobial
effect of Kombucha analogue. LWT - Food Science and
Technology. 47, 71-77.
- Bronner W.E. and Beecher G.R. 1981. Method for
determining the content of catechins in tea infusions by high
performance liquid chromatography. Journal of
Chromatography. 6805, 137–142.
- By Aamer A.A., Abdul-Hafeez M.M. and Sayed S.M. 2015.
Minimum inhibitory and bactericidal concentrations (MIC &
MBC) of Honey and bee propolis against multidrug resistant
(mdr) Staphylococcus spp. isolated from bovine clinical mastitis.
Global Journal of Science Frontier Research: D Agriculture and
Veterinary. 15, 2 Version 1.0 .
- Chikezie I.O., 2017. Determination of minimum inhibitory
concentration (MIC) and minimum bactericidal concentration
(MBC) using a novel dilution tube method. African Journal of
Microbiology Research. 11(23), 977-980.
- Chu S.C. and Chen C. 2006. Effects of origins and
fermentation time on the antioxidant activities of Kombucha.
Food Chemistry. 98, 502–507.
- Cruz-Gálvez A.M., Castro-Rosas J., Rodríguez-Marín M.L.,
Cadena-Ramírez A., Tellez-Jurado A., Tovar-Jiménez X.,
Chavez-Urbiola E.A., Abreu-Corona A. and Gómez-Aldapa C.A.
2018. Antimicrobial activity and physicochemical
characterization of a potato starch-based film containing
acetonic and methanolic extracts of Hibiscus sabdariffa for use
in sausage. LWT Food Science and Tecnology. 93, 300-305.
https://doi.org/10.1016/j.lwt.2018.02.064.
- Dipti P., Yogesh B., Kain A.K., Pauline T., Anju B., Sairam,
M., Singh B., Mongia S.S., Kumar G.I. and Selvamurthy W.
2003. Lead induced oxidative stress: beneficial effects of
Kombucha tea. Biomedical and Environmental Sciences. 16,
276–282.
- Dutta D. and Gachhui R. 2006. Novel nitrogen-fixing
Acetobacter nitrogenifigens spp nov., isolated from Kombucha
tea. Int. J. Syst. Evol. Microbiol. 56, 1899-1903.
- El-Mahmood M.A., 2009. Antibacterial efficacy of stem
bark extracts of Mangifera indica against some bacteria
associated with respiratory tract infections. Sci Res Essays.
4(10), 1031-1037.
- El-Salam S.S.A., 2012. 16S rRNA gene sequence detection
of acetic acid bacteria isolated from tea Kombucha. New York
Sci. J. 5, 55-61.
- Frank G., 1995. Kombucha, healty beverage and natural
remedy from the far east, Great Britain Published, Wilhelm
Ennsthaler, Steyr.
- Jayabalan R., Malba_sa R.V., Lon_car E.S., Vitas J.S. and
Sathishkumar M. 2014. A review on Kombucha tea -
microbiology, composition, fermentation, beneficial effects,
toxicity, and tea fungus. Compr. Rev. Food Sci. Food Saf. 13,
538 - 550.
- Jayabalan R., Malbaša R. and Sathishkumar M. 2016.
Kombucha. reference module in food sciences. Elsevier 1–8.
- Kim E.S., Liang Y.R., Jin J., Sun Q.F., Lu J.L., Du Y.Y. and
Lin D.C. 2007. Impact of heating on chemical compositions of
green tea liquor. Food Chemistry. 103, 1263-1267.
- Marsh A.J., O'Sullivan O., Hill C., Ross R.P. and Cotter P.D.
2014. Sequence-based analysis of the bacterial and fungal
compositions of multiple Kombucha (tea fungus) samples. Food
Microbiol. 38, 171-178.
- Jayabalan R.S., Marimuthu S. and Swaminathan K. 2007.
Changes in content of organic acids and tea polyphenols during
kombucha tea fermentation, Food Chemistry 102, 392–398.
- Reva ON, Zaets I.E., Ovcharenko L.P., Kukharenko O.E.,
Shpylova S.P., Podolich O.V., de Vera J.-P. and Kozyrovska N.O.
2015. Metabarcoding of the Kombucha microbial community
grown in different microenvironments. Amb. Express 5, 35.
- Roussin M.R., 1996. Analyses of Kombucha ferments:
report on growers. Salt Lake City, Utah: Information Resources,
LC.
- Sreeramulu G., Zhu Y. and Knol K. 2001. Characterization
of antimicrobial activity in Kombucha fermentation. Acta
Biotechnologica. 21,49- 56.
- Sreeramulu G., Zhu Y. and Knol W., 2000. Kombucha
fermentation and its antimicrobial activity. J. Agric. Food Chem.
48, 2589 - 2594.
- Stoner D.G. and Mukhtar H., 1995. Polyphenols as cancer
chemopreventive agents. Journal of Cellular Biochemistry. 22,
169–180.
- Stoner D.G. and Mukhtar H., 1995. Polyphenols as cancer
chemopreventive agents. Journal of Cellular Biochemistry. 22,
169–180.
- Su Y.L., Leung L.K., Huang Y. and Chen Z., 2003. Stability
of tea theaflavins and catechins. Food Chemistry. 83, 189–195.
- Trovatti E., Serafim L.S., Freire C.S.R., Silvestre A.J.D. and
Neto C.P. 2011. Gluconacetobacter sacchari: an efficient
bacterial cellulose cell-factory. Carbohydr. Polym. 86, 1417 -
1420.
- Vitasa J.S., Cvetanovića A.D., Maškovićb P.Z., ŠvarcGajića
J.V. and Malbašaa R.V. 2018. Chemical composition and
biological activity of novel types of Kombucha beverages with
yarrow. Journal of Functional Foods. 44, 95–102.
- Yang S.C., Maliakal P. and Meng X. 2002. Inhibition of
carcinogenesis by tea. Annual Review of Pharmacology and
Toxicology. 42, 25–54.
- Yang S.C., Prabhu S. and Landau J. 2001. Prevention of
carcinogenesis by tea polyphenols. Drug Metabolism Reviews.
33(3), 237–253.