Aziz BULBUL, Recep ASLAN, H. Ahmet CELİK, Gulcan AVCİ, Fatih FİDAN

Ovariektomize ratlarda ovaryum steroidlerinin oksidatif stres üzerine etkisi

Amaç: Ovariektomi yapılmış ratlarda östrojen ve progesteron hormonlarının beraber veya ayrı uygulanmasının lipid peroksidasyon, antioksidatif aktivite (AOA) ile β-karoten, vitamin A ve C düzeyine olan etkisinin belirlenmesi amaçlandı. Gereç ve Yöntem: Araştırma overioktemi yapılmış ve her biri 10 rattan oluşan kontrol ve 3 deneme grubunda yürütüldü. Kontrol grubuna susam yağı, progesteron grubuna progesteron 2 mg/rat/gün, östrojen grubuna 17β-östradiol 10 mg/rat/gün, östrojen + progesteron grubuna 17β-östradiol 10 mg/rat/gün + progestagen 2 mg/rat/gün olarak 10 gün uygulandı. En son uygulamadan 2 saat sonra genel anestezi altındaki ratlardan alınan kan örneklerinde malondialdehit (MDA), AOA, β-karoten, vitamin A ve C düzeyleri ölçüldü. Bulgular: Tam kan MDA düzeylerinin kontrol gruba göre östrojen grubunda azaldığı, ancak diğer deneme gruplarında fark bulunmadığı tespit edildi. Plazma AOA düzeyinin östrojen ve östrojen + progesteron gruplarında kontrol ve progesteron gruplarına göre yüksek (p

Anahtar Kelimeler:

Lipid peroksidasyon, östrojen, progesteron, vitamin A, vitamin C

Effects of ovarian steroids on oxidative stress in overiectomized rats

Aim: Determining the effects of combined or separate estrogen and progesterone administration on lipid peroxidation, antioxidative activity (AOA), β-carotene, vitamin A and C levels in overiectomized rats. Materials and Methods: The study was conducted on 1 control and 3 trial groups each consisted of 10 overiectomized rats. Control group received sesame oil while progesterone group received 2 mg/rat/day progesterone, estrogen group 10 mg/rat/day 17β-estradiol and estrogen + progesteron group 10 mg/rat/day 17β-estradiol+ 2 mg/rat/day progesterone for 10 days. Blood samples of rats were collected under general anesthesia after 2 hours following last administration and checked for malondialdehyde (MDA), AOA, β-carotene, vitamin A, and C levels.Results: Whole blood MDA levels in estrogen group were decreased as compared to control group while remained same in others. Plasma AOA levels of estrogen and estrogen + progesterone groups were higher (p<0.05) than control and progesterone groups. Plasma β-carotene levels were decreased in all trial groups when compared to control. Vitamin C levels of all groups were higher as compared to control group and were highest in estrogen + progesterone group.Conclusion: It can be concluded that estrogen decreases lipid peroxidation and increases antioxidant capacity besides effects positively the levels of antioxidant vitamins, particularly showing that mentioned level of estrogen act as an antioxidant.

Keywords:

Lipid peroxidation, estrogen, progesterone, vitamin A, vitamin C,

PDF

___

Ayers S, Abplanalp W, Liu JH, Subbiah MTR, 1998. Mechanisms involved in the protective effects of estradiol-17ß on lipid peroxidation and DNA damage. Am J Physiol, 274, 1002-1008.
Bednarek-Tupikowska G, Tupikowski K, Bidzińska B, Bohdanowicz-Pawlak A, Antonowicz-Juchniewicz J, Kosowska B, Milewicz A, 2004. Serum lipid peroxides and total antioxidant status in postmenopausal women on hormone replacement therapy. Gynecol Endocrinol, 19, 57-63.
Bhavnani BR, 2003. Estrogens and menopause: pharmacology of conjugated equine estrogens and their potential role in the prevention of neurodegenerative diseases such as Alzheimer’s. J Steroid Biochem, 85, 473-482.
Borras C, Sastre J, Garcia-Sala D, Lloret A, Pallardo FV, Vina J, 2003. Mitochondria from females exhibit higher antioxidant gene expression and lower oxidative damage than males. Free Radic Biol Med, 34, 546-552.
Bulbul A, Celik HA, Sireli M, Avci G, Civelek T, 2008. Blood nitric oxide and ovarian streoids levels during the cycle stages in Brown Swiss cows. Ankara Univ Vet Fak Derg, 55, 155-159.
Bulbul A, Yagci A, Altunbas K, Sevimli A, Celik HA, Karadeniz A, Akdag A, 2007. The role of nitric oxide in the effects of ovarian steroids on spontaneous myometrial contractility in rats. Theriogenology, 68, 1156-1168.
Celik HA, Avci G, Aydin I, Bulbul A, Bulbul T, 2009. Effect of β-carotene on ovarium functions and ovsynch success in repeat breeder cows. Kafkas Univ Vet Fak Derg, 15, 1-8.
Delibasi T, Kockar C, Celik A, Kockar O, 2006. Antioxidant effects of hormone replacement therapy in postmenopausal women. Swiss Med Wkly, 5, 510-514.
Dlugosz A, Roszkowska A, Zimmer M, 2009. Oestradiol protects against the harmful effects of fluoride more by ıncreasing thiol group levels than scavenging hydroxyl radicals. Basic Clin Pharmacol, 105, 366-373.
Draper HH, Hardley M, 1990, Malondialdehyde determination as index of lipid peroxidation. Methods Enzymol, 186, 421-431.
Ha BJ, Lee SH, Kim HJ, Lee JY, 2006. The role of Salicornia herbacea in ovariectomy-induced oxidative stress. Biol Pharm Bull, 29, 1305-1309.
Haliloglu S, Baspinar N, Serpek B, Erdem H, Bulut Z, 2002. Vitamin A and b-carotene levels in plasma, corpus luteum and follicular fluid of cyclic and pregnant cattle. Reprod Dom Anim, 37, 96-99.
Halliwell B, 1997. Antioxidants and human disease: A general introduction. Nutr Reviews, 55, 44-52.
Katz D, Mazor D, Dvilansky A, Meyerstein N, 1996. Effect of radiation on red cell membrane and intra cellular oxidativedefense system. Free Radic Res, 24, 199-204.
Kinlay S, Selwyn AP, Delagrange D, Creager MA, Libby P, Ganz P, 1996. Biological mechanisms for the clinical success of lipid-lowering in coronary artery disease and the use of surrogate end-points. Curr Opin Lipid, 7, 389-397.
Koracevic D, Koracevic G, Djordjevic V, Andrejevic S, Cosic V, 2001. Method for the measurement of antioxidant activity in human fluids. J Clin Pathol, 54, 356-361.
Kway A, 1978. A simple colorimetric method for ascorbic acid determination in blood plasma. Clin Chim Acta, 86, 153-157.
Luck MR, Jeyaseelan I, Scholes RA, 1995. Ascorbic acid and fertility. Biol Reprod, 52, 262-266.
Lutoslawska G, Tkaczyk J, Panczenko-Kresowska B, Hubner-Wozniak E, Skierska E, Gajewski AK, 2003. Plasma TBARS, blood GSH concentrations, and erythrocyte antioxidant enzyme activities in regularly menstruating women with ovulatory and anovulatory menstrual cycles. Clin Chim Acta, 331, 159-163.
Maenpaa PH, Pirhonen A, Koskinen E, 1988. Vitamin A, E and D nutrition in mares and foals during the winter seasons; effects of feeding two different vitamin-mineral concentrate. J Anim Sci, 66, 1424-1429.
Makover A, Soprano DR, Wyatt ML, Goodman DS, 1989. An in situ-hybridization study of the localization of retinolbinding protein and transthyretin messenger RNAs during fetal development in the rat. Differentiation, 40, 17-25.
Michos C, Kiortsis DN, Evangelou A, Karkabounas S, 2006. Antioxidant protection during the menstrual cycle: the effects of estradiol on ascorbic-dehydroascorbic acid plasma levels and total antioxidant plasma status in eumenorrhoic women during the menstrual cycle. Acta Obstet Gynecol Scand, 85, 960-965.
Mukai K, Daifuku K, Yokoyama S, Nakano M, 1990. Stoppedflow investigation of antioxidant activity of estrogens in solution. Biochim Biophys Acta, 1035, 348-352.
Ozgonul M, Oge A, Sezer ED, Bayraktar F, Sozmen EY, 2003. The effects of estrogen and raloxifene treatment on antioxidant enzymes in brain and liver of ovarectomized female rats. Endocr Res, 29, 183-189.
Rifici VA, Khachadurian AK, 1992. The inhibition of lowdensity lipoprotein oxidation by 17-beta estradiol. Metabolism, 41, 1110-1114.
Ruiz-Larrea MB, Martin C, Martinez R, Navarro R, Lacort M, Miller NJ, 2000. Antioxidant activities of estrogen against aqueous and lipophilic radicals; differences between phenol and catechol estrogens. Chem Phys Lip, 105, 179-188
Sack MN, Rader DJ, Cannon RO, 1994. Oestrogen and inhibition of oxidation of low-density lipoproteins in postmenopausal women. Lancet, 343, 269-270.
Schweigert FJ, Lutterbach A, Rambeck WA, Zucker H, 1986. Vitamin A and β-carotene concentrations in bovine follicular fluid in relationship to follicle size. J Vet Med A, 33, 360-364
Soprano DR, Soprano KJ, Goodman DS, 1986. Retinol-binding protein and thransthyretin mRNA levels in visceral yolk sac and liver during fetal development in the rat. Proc Natl Acad Sci, 83,7330-7334.
Strehlow K, Rotter S, Wassmann S, Adam O, Grohe C, Laufs K, Bohm M, Nickenig G, 2003. Modulation of antioxidant enzyme expression and function by estrogen. Circ Res, 93, 170-177.
Subbiah MTR, Kessel B, Agrawal M, Rajan R, Abplanalp W, Rymaszewski Z, 1993. Antioxidant potantial of specific estrogens on lipid peroxidation. J Clin Endocrinol Metab, 77, 1095-1997.
Suzuki J, Katoh NA, 1990. Simple and cheap methods for measuring serum vitamin-A in cattle using only a spectrophotometer. Nippon Juigaku Zasshi, 52, 1281-1283.
Wassmann K, Wassmann S, Nickenig G, 2005. Progesterone antagonizes the vasoprotective effect of estrogen on antioxidant enzyme expression and function. Circ Res, 97, 1046-54.
Wefers H, Sies H, 1988. The protection by ascorbate and glutatione against lipid peroxidation is dependent on vitamin E. Eur J Biochem, 174, 353–357.
Whitman MM, Harnish DC, Soprano KJ, Soprano DR, 1990. Retinol-binding protein mRNA is induced by estrogen in the kidney but not in the liver. J Lipid Res, 31, 1483- 1490.
Yagi K, Komura S, 1986. Inhibitory effect of female hormones on lipid peroxidation. Biochem Int, 13, 1051-1055.
Yuce A, Aksakal M, 2005. Ratlarda homosisteinin oksidanantioksidan sistem ve koroner damarlarda oluşurduğu değişiklikler üzerine melatoninin etkisi. FÜ Saglık Bil Derg, 20, 51-59.