Leslie A. MACLAREN, Todd R BİLBY, Frank MİCHEL, Aydin GUZELOGLU, Charles R. STAPLES, William W. THATCHER.

İnek somatotropini uygulaması veya omega-3 zengini balık yağı (FO) ile beslenen ineklerde peroxisome proliferator-activated reseptörlerinin (PPAR) ekspresyonunu etkiler

Amaç: Çalışmanın amaçları, holştayn ırkı ineklerin rasyonuna 90 gün boyunca zenginleştirilmiş balık yağı formunda uzun zincirli omega-3 çoklu doymamış balık yağı katılmasının veya ovulasyon günü ve 11 gün sonrasında iki kere inek somatotropin enjeksiyonunun endometriumunda PPAR ekspresyonu ve aktivasyonunu araştırmaktır. Gereç ve Yöntem: Laktasyonda olmayan inekler siklik, siklik-bST, gebe veya gebe-bST olmak üzere 4 gruba ayrıldı. Laktasyondaki inekler ise siklik, siklik-bST, gebe, gebe-bST, siklik-FO veya siklik-FO-bST olmak üzere 6 gruba ayrıldı.Bulgular: Northern ve Western blot analizleri PPARα ve PPARδ’nın ovulasyon sonrası 17. günde endometriumda ekspre edildiğini belirlendi ancak PPARγ tespit edilemedi. bST uygulaması sadece gebelerde PPARδ mRNA ekspresyonunu arttırdı, bu etki embriyo tarafından düzenlenmiş olabilir. Laktasyondaki gebe ineklerin endometriumundaki bST’ya bağlı PPARα mRNA miktarındaki artış bST etkisinin gebelik durumuna bağlı olduğunu vurgulamaktadır. Rasyona balık yağı eklenmesi PPARδ mRNA miktarını azaltırken, PPARα mRNA miktarı üzerine bir etkisi olmadı. PPARδ proteini luminal epitelde, glandular epitelde, subepitel stromada ve az miktarda da adluminal stromada belirlendi. Anti-PPARδ reaksiyonu bST uygulaması ve balık yağı beslemesine bağlı olarak gebe ineklerde azaldı. Öneri: bST uygulaması ve balık yağı beslemesi endometrial PPARα ve PPARδ ekspresyonun laktasyondaki sütçü ineklerde etkilemektedir.

Anahtar Kelimeler:

İnek, endometrium, PPAR, bST, balık yağı

Supplementation of dairy cows with bovine somatotropin or omega-3 rich fish oil affects the endometrial expression of peroxisome proliferator-activated receptors

Aim: The study objectives were to determine whether dietary supplementation with long chain omega-3 polyunsaturated fatty acids in the form of enriched fish oil (FO) for 90 days or treatment with bovine somatotropin (bST) at the time of ovulation and 11 days post-ovulation influenced PPAR expression and activation in bovine endometrium in Holstein cows.Materials and Methods: Non-lactating cows were assigned to one of four treatments: cyclic, cyclic-bST, pregnant or pregnant-bST. Lactating cows were assigned to one of six treatments: cyclic, cyclic-bST, pregnant, pregnant-bST, cyclic-FO or cyclic-FO-bST.Results: Northern and Western blot analyses indicated that PPARα and PPARδ, but not PPARγ, are expressed in endometrium from all cows at day 17 post-ovulation. Treatment with bST is associated with increased PPARδ mRNA abundance in pregnant but not cyclic cows, suggesting that the effect may be mediated by the embryo. Increased abundances of PPARα mRNA are observed in response to bST during pregnancy in lactating cows but not in non-lactating cows, highlighting the importance of lactation status in determining bST response. Fish oil supplementation is associated with reduced PPARδ mRNA abundance, but did not affect steady-state PPARα mRNA abundance. PPARδ protein is expressed in the luminal epithelium, glandular epithelium, subepithelial stroma and to a lesser extent in the adluminal stroma. Anti-PPARδ reactivity is reduced in response to bST and fish oil treatments in pregnant cows.Conclusion: bST and fish oil treatments affect endometrial PPARα and PPARδ expression in lactating dairy cows.

Keywords:

Cow, endometrium, PPAR, bST, fish oil,

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