Dilek Öztürk, Hülya Türütoğlu, Özlem Şahan

Bir veteriner fakültesinin kliniklerinde metisiline dirençli Staphylococcus aureus (MRSA) kolonizasyonu

Amaç: Metisiline dirençli S. aureus (MRSA), birçok antibiyotiğe direnci nedeniyle, insan ve veteriner hekimliğinde enfeksiyonların tedavisinde güçlüklere neden olan önemli bir zoonotik ajandır. Bu çalışmada, bir veteriner fakültesinin kliniklerinde çalışan veteriner hekimler, personel ve öğrencilerin el ve burun mukozaları ile klinik ortamından alınan sıvap örneklerinde MRSA kolonizasyonu araştırıldı. Gereç ve Yöntem: Bu amaçla, 16 veteriner hekim, 2 klinik personeli ve 17 öğrencinin herbirinden alınan dört (iki el ve burun mukozası) ve klinikte el ile temasının sık olduğu düşünülen 41 farklı çevresel yüzeyden sıvap örnekleri toplandı. Bulgular: Yüz on sekiz S. aureus izolatının 75’i (%63.6; 34 veteriner hekim, 8 personel, 24 öğrenci, 9 çevresel yüzey) disk difüzyon testi ile metisiline fenotipik olarak dirençli bulundu. On dört veteriner hekim ve 7 öğrenciden alınan örneklerin en az birinde metisiline fenotipik dirençli S. aureus taşıyıcılığı belirlenirken, iki personelden alınan örneklerin tümünde metisiline fenotipik dirençli S. aureus tespit edildi. Veteriner hekimler (13 izolat), personel (1 izolat) ve öğrencilerden (10 izolat) izole edilen 24 (%20.3) S. aureus izolatında, metisiline direnci kodlayan mecA geni pozitif bulunurken, çevresel yüzeylerden izole edilemedi. Öneri: MRSA kolonizasyonunun klinikte çalışan insanlar ve öğrencilerde yüksek olduğu, insanlar ve hayvanlar arasındaki geçiş riski nedeniyle sanitasyon önlemlerinin, özellikle de personel hijyeninin gerekli olduğu kanısına varıldı.

Anahtar Kelimeler:

Kolonizasyon, MRSA, Staphylococcus aureus, veteriner klinikleri

Methicillin resistant Staphylococcus aureus (MRSA) colonization at clinics of a Faculty of Veterinary Medicine

Aim: Methicillin resistant Staphylococcus aureus (MRSA) is an important zoonotic agent causing difficulties in treatment of the infections in human and veterinary medicine, due to multiple antibiotic resistances. In this study, the colonization of MRSA was investigated by the swabbing samples from the skin and nasal mucosa of veterinarians, personnel and students and from the environment at clinics of a Faculty of Veterinary Medicine. Materials and Methods: For this purpose, samples were obtained from 16 veterinarians, 2 clinic personnel and 17 students that each was sampled 4 times and from 41 different environmental surfaces thought to be common sites of hand contact. Results: Out of 118 S. aureus isolates, 75 (63.6%; 34 veterinarians, 8 personnel, 24 students and 9 environmental surfaces) were found phenotypically resistant to methicillin by a disk diffusion test. All samples taking from two personnel were colonized with phenotypic resistant S. aureus, while the 14 veterinarians and 7 students sampled were carried the phenotypic resistant S. aureus at least to one of samples. Although 24 (20.3%) S. aureus isolated from veterinarians (13 isolates), personnel (1 isolate) and students (10 isolates) were determined to be positive for mecA gene encoded resistance to methicillin, mecA-positive isolates can not be isolated from environmental surfaces at clinics. Conclusions: It has been determined that MRSA colonization has high in working people and students at the clinic, thus standard sanitation measures, particularly personnel hygiene, are required because of the risk of transmission between humans and animals.

Keywords:

Colonization, MRSA, Staphylococcus aureus, veterinary clinics,

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