Murat Sevik, Oguzhan Avci

Atık küçük ruminant fötuslarında Bovine Herpes Virus-1’in moleküler olarak araştırılması

Bu çalışmada atık küçük ruminant fötus dokularında, Bovine Herpes Virus-1 (BoHV-1) varlığının PCR tekniği kullanılarak belirlenmesi amaçlandı. Bu amaçla İç Anadolu Bölgesi’ndeki farklı illerden (Konya, Aksaray ve Niğde), BoHV-1 seropozitif sığırlar ile bir arada yaşayan, her biri farklı sürülerdeki küçük ruminantlara ait atıklardan (43 koyun ve 23 keçi) iç organ (akciğer, karaciğer ve dalak, toplam n=198) örnekleri toplandı. Glikoprotein D (gD) genine spesifik polimeraz zincir reaksiyonu (PZR) kullanılarak, BoHV-1 varlığı araştırıldı. Doku örneklerinin hiçbirinde BoHV-1 varlığı tespit edilemedi. Elde edilen sonuçlara dayanarak, İç Anadolu Bölgesi’nde BoHV-1’in küçük ruminant abort vakalarında rol oynamadığı ifade edilebilir.

Anahtar Kelimeler:

Bovine Herpesvirus 1, küçük ruminant, abort, PCR

Molecular investigation of Bovine Herpesvirus Type 1 from aborted small ruminant foetuses

The aim of this study was carried out to determine the presence of Bovine Herpes Virus-1 (BoHV-1) in aborted small ruminant foetus’ tissues by using Polymerase Chain Reaction (PCR) technique. For this purpose, aborted tissue samples (liver, lung, and spleen, totally n=198) were collected from small ruminants (43 sheep and 23 goats), each from different flocks and lived together with BoHV-1 seropositive cattle, in Central Anatolia region (Konya, Aksaray, and Niğde provinces) of Turkey. Glycoprotein D (gD) gene based PCR was used for the detection of BoHV-1. All tissue samples were detected as negative for BoHV-1 DNA. According to results, it can be expressed that BoHV-1 is not play a role in abortion cases in small ruminants in Central Anatolia Region.

Keywords:

Bovine Herpesvirus 1, small ruminant, abortion, PCR,

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