Deniz YENİ, Mustafa GÜNDOĞAN, Fatih AVDATEK

Kısa Süreli Saklanan Koç Spermasına İlave Edilen Sığır Serum Albumininin (BSA) Farklı Dozlarının Sperma Kalitesi ve DNA Hasarı Üzerine Etkisi

Bu çalışmanın amacı kısa süreli (5 °C)’de saklanan Merinos koç spermasında 0, 24, 48 ve 72. saatlerde sığır serumalbümininin (BSA) sperma kalitesi ve DNA hasarı üzerindeki etkilerini belirlemektir. Ejekülatlar beş baş Merinos ırkı koçtansuni vajen ile toplandı ve değerlendirmeler 37 °C'de yapıldı. Tüm çalışma altı kez tekrar edildi. Toplanan koç sperması örnekleridörde bölündü ve Tris temelli sulandırıcı ile sulandırılan spermaya (0, 2, 4 ve 6 mg/ml) dozlarında BSA eklendi ve motilite,anormal spermatozoon oranı, plazma membran bütünlüğü ve canlılık, akrozom bütünlüğü ve DNA hasarı (COMET testi)belirlendi. Çalışma gruplarından 4 mg/ml BSA içeren grup, 72 saatlik depolamaya kadar, kontrol ile karşılaştırıldığında dahayüksek motilite yüzdeleri, plazma membran bütünlüğü ve canlılık gösterdi (P

The Effect of Different Concentrations of Bovine Serum Albumin on Sperm Quality and DNA Damage in Short Term Stored Ram Semen

In the present research the aim was emphasized to find out the effects of bovine serum albumin (BSA) on quality of sperm and DNA damage at 0, 24 th, 48 th and 72 th hours in Merino ram semen atshort term preservation (5°C). Ejaculates were obtained from five Merino Rams which were collected by artificial vagina and all the samples were pooled at 37°C for detection. All the procedures were applied six times concurrently. Different concentrations (0, 2, 4 and 6 mg/ml) of BSA with Tris-based extender was used for dilution on semen samples. Sperm motility, abnormality, plasma membrane integrity, acrosome integrity and % DNA in tail (COMET assay) content were measured and analyzed. The extender with 4 mg/ml of BSA had both higher motility percentages and plasma membrane integrity, when compare with the control, up to 72 hours (P

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