Harun ALBAYRAK, Emre ÖZAN, Hatice Mahur TURAN, Yavuz Selim MEMİŞ, Abdullah CAVUNT

Efficiency of three pprv vaccines commercially available in Turkey

Koyun ve keçilerde ticari üç farklı küçük ruminant vebası virus (PPRV) aşısının etkinliği antikor cevabına dayalı birmetot olan competitive enzyme-linked immunosorbent assay (c-ELISA) ile kontrol edildi ve sonuçlar virus nötralizasyon testi(VNT) ile doğrulandı. Söz konusu çalışma, Haziran 2010 ile Ocak 2011 tarihleri arasında yapıldı. Her iki cinsiyeti içeren 150koyun ve 50 keçiden oluşan çalışma grubu, Grup 1, 2 ve 3 olmak üzere üç gruba ayrıldı. Her gruba farklı bir aşı uygulamasıyapıldı. Serum örnekleri aşılama öncesi (0. gün) ve aşılama sonrasında (18. gün ve 8. ay) olmak üzere toplam üç kez alındı.Araştırma sonuçlarına göre, keçilerde PPRV’ye karşı antikor cevabı şekillenirken, koyunların çoğunda antikor cevabınınınşekillenmediği tespit edildi.

Türkiye`de ticari olarak kullanılan üç farklı pprv aşısının etkinliği

The efficiencies of three different commercially available Peste des petits ruminants virus (PPRV) vaccines wereevaluated on the basis of the antibody response surveyed by competitive enzyme-linked immunosorbent assay (c-ELISA)and the results were confirmed by virus neutralization test (VNT) in sheep and goats. The study was conducted during theperiod from June 2010 to January 2011 to determine the antibody response against three different commercially availablehomologous PPR (Nigeria 75/1) vaccines. A total of 150 sheep and 50 goats of both genders were divided into three groups,as Group 1, 2 and 3. Each group was inoculated one out of three vaccines. A total of three samples of were collected threetimes from all the groups at pre- (day zero) and post-vaccination (18thdays and 8thmonths). The results indicate thattreated goats developed antibodies after the vaccination, while the most of sheep did not have any antibodies for PPRV.

PDF

___

Albayrak H., Alkan F., 2009. PPR virus infection on sheep in blacksea region of Turkey: Epidemiol ogy and diagnosis by RT-PCR and virus isola tion. Vet. Res. Commun., 33, 241-249.
Anderson J., Mckay JA., Butcher RN., 1991. The use of monoclonal antibodies in competitive ELISA for the detection of anitbodies to rinderpest and peste des petits ruminants viruses. In ‟The seromonitoring of rinderpest throughout Af rica—Phase one”, Ed., MH Jeggo, Proceedings of the final research coordination meeting of the FAO/IAEA/SIDA/OUA/IBAR/PARC coordi nated research programme, Bingerville, Ivory Coast, IAEA-TECDOC, 623, 43–53.
Anonymous, 2009. Manual, chapter 2.7.11 in the manual of diagnostic tests and vaccines for ter restrial animals. Peste des petits ruminants. http://www.oie.int/eng/normes/MMANUAL/2 008/pdf/2.07.11_PPR.pdf.[Acces:20.05.2010]
Baron MD., Parida S., Oura CAL., 2011. Peste des petits ruminants: a suitable case for eradica tion? Vet. Rec., 169, 16-21, doi: 10.1136/ vr.d3947.
Barrett T., Banyard AC., Diallo A., 2005. Molecular Biology of the Morbilliviruses. Virus plagues of large and small ruminants, Elsevier, Amster dam.
Chandran D., Bhaktavatsala RK., Vijayan SP., Sugumar P., Rani GS., Kumar PS., Rajendra LS., Srinivasan V., 2010. MVA recombinants ex pressing the fusion and hemagglutinin genes of PPRV protects goats against virulent challenge. Indian J. Microbiol., 50, 266-274.
Diallo A., 1988. Rinderpest and peste des petits ruminants; constant threats to animal farming in many developing countries. Impact Sci. Soci ety, 150, 179–192.
Diallo A., Minet C., Goff CL., Berhe G., Albina E., Libeau G., Barrett, T., 2007. The threat of peste des petits ruminants: progress in vaccine development for disease control. Vaccine, 25, 5591-5597.
Gür S., Albayrak H., 2010. Seroprevalance of peste des petits ruminants (PPR) in goitered gazelle (Gazella subgutturosa subgutturosa) in Turkey. J. Wildlife Dis., 46, 673-677.
Intizar M., Ahmad MD., Anjum AA., Hanif A., 2009. Comparative efficacy of peste des petits rumi nants (PPR) vaccines available in Pakistan in sheep and goats. Pak. Vet. J., 29, 202-205.
Khan HA., Siddique M., Arshad MJ., Khan QM., Rehman SU., 2007. Sero-prevalence of peste des petits ruminants (PPR) virus in sheep and goats in Punjab province of Pakistan. Pak. Vet. J., 27, 109-112.
Kwiatek O., Minet C., Grillet C., Hurard C., Carlsson E., Karimov B., Albina E., Diallo A., Libeau G., 2007. Peste des petits ruminants (PPR) out- break in Tajikistan, J. Comp. Pathol., 136, 111- 119.
Mahapatra M., Parida S., Baron MD., Barrett T., 2006. Matrix protein and glycoproteins F and H of Peste-des-petits-ruminants virus function better as a homologous complex. J. Gen. Virol., 87, 2021-2029.
Ozmen O., Kale M., Haligur M., Yavru, S., 2009. Pathological, serological, and virological find ings in sheep infected simultaneously with bluetongue, peste des petits ruminants, and sheeppox viruses. Trop. Anim. Health Pro., 41, 951-958.
Sarkar J., Sreenivasa BP., Singh RP., Dhar P., Bandyopadhyay SK., 2003. Comparative effi cacy of various chemical stabilizers on the thermostability of a live-attenuated peste des petits ruminants (PPR). Vaccine, 21, 4728- 4735.
Siddique MP., Rahman MB., Chowdhury SMZH., Kafi MA., Alam MS., 2006. Determination of effi cacy of thermostable ppr live homologous vac cine incubated at room temperature for 14 days. Bangladesh J. Vet. Med., 4, 43-46.
Singh RP., Sreenivasa BP., Dhar P., Shah LC., Bandyopadhyay, SK., 2004. Development of a monoclonal antibody based competitive-ELISA for detection and titration of antibodies to peste des petits ruminants (PPR) virus. Vet. Microbiol., 98, 3-15.
Worrwall EE., Litamoi JK., Seck B., Ayelet M., Xerovac G., 2001. An ultra rapid method for the dehydration and preservation of live at tenuated rinderpest and peste des petits rumi nants vaccines. Vaccine, 19, 834-839.