Mehmet Kürşad KARADAĞ, Hakan Hadi KADIOĞLU

NİGELLA SATİVA EKSTRESİNİN CAS: 490-91-5 DENEYSEL SUBARAKNOİD KANAMAYA BAĞLI VASKÜLER DEĞİŞİKLİKLER ÜZERİNE OLAN ETKİSİNİN IŞIK MİKROSKOBİK VE STEREOLOJİK İNCELENMESİ

Amaç: Bu çalışmada Nigella Sativa Ekstresinin CAS:490-91-5, ratlarda oluşturulan deneysel subaraknoid kanama modelinde vazospazm üzerine etkileri incelendi. Gereç ve Yöntem: 60 adet erkek Wistar cinsi albino rat, altı gruba ayrıldı. Grup 1 (kontrol), grup 2 (sham), grup 3 (subaraknoid kanama sonrası birinci saatte dekapitasyon), grup 4 (subaraknoid kanama sonrası 48.saatte dekapitasyon), grup 5 (subaraknoid kanama öncesi thymoquinone verilip, 6 saat sonrasında subaraknoid kanama oluşturulup bundan 1 saat sonra dekapitasyon) ve grup 6 (subaraknoid kanama sonrası birinci, 24. ve 48.saatlerde thymoquinone verilip sonrasında dekapitasyon) olarak tanzim edildi. Atatürk Üniversitesi Tıp Fakültesi Biyokimya Laboratuvarı’nda dimetil sülfoksit yardımı ile dilüe edilerek hazırlanan thymoquinone, 5mg/kg dozunda intraperitoneal yolla verildi. İşlem sırasında 8 adet rat kaybı oldu. Kalan 52 adet ratın dekapitasyon işlemi sonrasında baziler arterlerin lümen alanları ve duvar kalınlıkları stereolojik yöntemle histopatolojik olarak incelendi. Bulgular: Subaraknoid kanama öncesi tek doz verilen (grup5) ve subaraknoid kanama sonrası 3 gün thymoquinone verilen (grup6) grupların her ikisi de hiç thymoquinone verilmeyen (grup3) gruba göre baziller arter lümen alanlarında genişleme istatistiksel olarak anlamlıdır. Arter duvar kalınlıkları thymoquinone verilen (grup6 ve grup5) gruplar ile thymoquinone verilmeyenler (grup3, grup4) arasındaki fark istatistiksel olarak anlamlı bulunmuştur. Bu sonuç bize ilaç verilme ve verilmeme durumu arasında duvar kalınlığı açısından farklılık olduğunu ortaya koymuş, ilaç verilen gruplarda duvar kalınlığı azalmıştır. Sonuç: Bu çalışmada elde edilen bulgular, thymoquinone’nun ratlarda subaraknoid kanama sonrası vazospazmı önleyici olabileceğini göstermektedir

Anahtar Kelimeler:

Nigella Sativa Ekstresi CAS:490-91-5, stereoloji, subaraknoid kanama, vazospazm

THE STEREOLOGICAL AND LIGHT MICROSCOPY INVESTIGATION OF THE EFFECT OF NIGELLA SATIVA EXTRACT CAS: 490-91-5 ON VASCULAR CHANGES IN EXPERIMENTAL SUBARACHNOID HEMORRHAGE MODEL

Objective: In this study, the effect of Nigella Sativa Extract CAS: 490-91-5 The effects on vasospasm in rats was investigated in an experimental subarachnoid hemorrhage model. Material and Method: 60 male Wistar albino rats were divided into 6 groups as follows; Group 1 (control), group 2 (sham), group 3 (decapitation, one hour after subarachnoid hemorrhage), group 4 (decapitation, 48 hours after subarachnoid hemorrhage), group 5 (6 hours prior to subarachnoid hemorrhage thymoquinone injection and one hour later after subarachnoid hemorrhage decapitation), group 6 (thymoquinone injection 1, 24 and 48 hours later subarachnoid hemorrhage and decapitation after the last injection). Thymoquinone that was prepared in the Atatürk University Biochemistry Laboratory via dilatation of dimethyl sulfoxide was injected intraperitoneally with the dose of 5mg/kg to the rats. 8 rats died during the procedure. The basilar artery lumen size and wall thickness of the remaining 52 rats were investigated with a stereological histopathologically approach after decapitation. Results: The widening of basilar artery lumen of group 5 (one dose of thymoquinone) and group 6 (3 doses of thymoquinone after subarachnoid hemorrhage) is statistically significantly increased than group 3 (no thymoquinone injection). The difference of artery wall thicknesses between thymoquinone injected groups (5 and 6) and non-thymoquinone injected groups (3-4) are also statistically significant. This result indicates that the thymoquinone effectively makes a significant difference to the wall thickness, thymoquinone injected groups have decreased wall thickness. Conclusion: The result of this study shows that thyquinone scans prevents vasospasm related to subarachnoid hemorrhage. Further studies that use this agent in clinical trials are needed to show the clinical effects of thymoquinone.

Keywords:

Nigella Sativa Extract CAS: 490-91-5, stereology, subarachnoid hemorrhage, vasospasms,

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