İki farklı tip tahribatsız portatif klorofil metre kullanarak Artvin’de yayılış gösteren sapsız meşede (Quercus, Fagaceae) klorofil tahmini

Bu çalışmada, SPAD-502Plus ve CCM-300 adlı optik klorofil içerik ölçer cihazlarından elde edilenklorofil içerik değerleri ile %80 aseton ile klorofil ekstraksiyon metodundan elde edilen klorofil içerikdeğerleri arasındaki ilişkinin saptanması amaçlanmıştır. Bu çalışma, sapsız meşenin, Quercus petraea(Mattuschka) Liebl. subsp. iberica (Steven ex Bieb.) Krassiln. alt türü üzerinde gerçekleştirilmiştir.Rakımı 889 m olan önceden belirlenmiş bir alandan benzer özelliklere sahip 11 ağaç seçilmiş, sağlıklıtoplam 44 yaprak üzerinde ölçümler gerçekleştirilmiştir. Yapraklara ait SPAD, CFR (klorofil floresans)değerleri ve %80 aseton ile klorofil ekstraksiyonu devamında yapılan spektrofotometrik okumalarsonucunda elde edilen bulgular ayrı ayrı değerlendirilmiştir. Ölçümler sonucunda, her üç ölçüm tipi(SPAD, CFR ve %80 aseton metodu) sonuçlarının kuvvetli ilişki sergilediği tespit edilmiştir. Elde edilenSPAD ve CFR değerlerinin %80 aseton ile ekstraksiyon metodundan elde edilen verileredönüştürülmesi amacıyla regresyon analizleri ile çeşitli eşitlikler oluşturulmuştur. Bu eşitliklerkullanılarak, SPAD ve CFR değerlerinin, kimyasal metodla tespit edilmiş klorofil içerik değerlerinedönüşümü mümkün olabilmektedir. Bu çalışmada, bu meşe türü için, birim alan başına yapraktoplam klorofil konsantrasyonu, SPAD (R2=0.9367) ve CFR (R2=0.8702) okumaları arasındaki ilişkinin,taze ağırlık başına yaprak toplam klorofil konsantrasyonuna göre (SPAD (R2=0.8264) ve CFR(R2=0.7847)) daha yüksek olduğu tespit edilmiştir. Bu sonuçlara göre, bu optik cihazları kullanarak,tercihen birim alan başına (mg cm-2), yaprak toplam klorofil içeriği çok kısa sürede ve kolaylıklayapraklara zarar vermeden belirlenebilir.

Estimation of chlorophyll in sessile oak (Quercus, Fagaceae) naturally distributed in Artvin province using two different types of non-destructive portable chlorophyll meter

In this study, it was aimed to determine the relationship between the chlorophyll content values obtained from SPAD-502Plus and CCM-300 optical chlorophyll content meters and the chlorophyll content values obtained from the chlorophyll extraction method using 80% acetone. This study was carried out on a subspecies of the sessile oak (Quercus petraea (Mattuschka) Liebl. subsp. iberica (Steven ex Bieb.) Krassiln.). 11 trees with similar characteristics were selected from a predefined area with an altitude of 889 m and measurements were made on a total of 44 healthy leaves. The leaf SPAD values, CFR (chlorophyll fluorescence) values and the results obtained from the spectrophotometric readings of the chlorophyll extraction with 80% acetone were evaluated separately. As a result of the measurements, it was determined that the results of all three measurement types (SPAD, CFR and 80% acetone method) showed a strong correlation. Various equations were formed by regression analyses in order to convert the obtained SPAD and CFR values to the values obtained from the 80% acetone method. Using these equations, it is possible to convert SPAD and CFR values into chlorophyll content values determined by chemical method. In this study, for this type of oak, the relationship between the leaf total chlorophyll concentration per unit area and SPAD (R2=0.9367) and CFR (R2=0.8702) readings was found higher than the leaf total chlorophyll concentration per fresh weight (SPAD (R2=0.8264) and CFR (R2=0.7847)). According to these results, by using these optical devices, preferably per unit area (mg cm-2), the leaf total chlorophyll content can be determined in a very short time and easily without damaging the leaves

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Ali S, Khan MA, Kim WC (2018) Pseudomonas veronii KJ mitigates flood stress-associated damage in Sesamum indicum L. Appl Biol Chem 61: 575-585 https://doi.org/10.1007/s13765-018-0392-2
Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiology 24: 1-15
Bielinis E, Jozwiak W, Robakowski P (2015) Modelling of the relationship between the SPAD values and photosynthetic pigments content in Quercus petraea and Prunus serotina leaves. Dendrobiology 73: 125-134
Curran PJ, Dungan JL, Gholz HL (1990) Exploring the relationship between reflectance red edge and chlorophyll content in slash pine. Tree Physiology 7: 33–48
Filella I, Serrano L, Serra J, Peñuelas J (1995) Evaluating wheat nitrogen status with canopy reflectance indices and discriminant analysis. Crop Science 35: 1400–1405
Fujimura S, Shi P, Iwama K, Zhang X, Gopal J, Jitsuyama Y (2009) Comparison of growth and grain yield of spring wheat in Lhasa, the Tibetan Plateau, with those in Sapporo, Japan. Plant Prod Sci 12: 116-123
Gamon JA, Surfus JS (1999) Assessing leaf pigment content and activity with a reflectometer. New Phytologist 143: 105–117
Gitelson AA, Buschmann C, Lichtenthaler HK (1999) The Chlorophyll Fluorescence Ratio F735/F700 as an Accurate Measure of Chlorophyll Content in Plants. Remote Sens Enviro 69: 296-302
Larcher W (1995) Physiological Plant Ecology, 3rd edn. Berlin, Germany: Springer.
Lichtenthaler HK (1987b) Chlorophyll and carotenoids, the pigments of photosynthetic biomembranes. Methods Enzymol 148: 350– 382
Marchetti CF, Skrabisova M, Galuszka P, Novak O, Causin HF (2018) Blue light suppression alters cytokinin homeostasis in wheat leaves senescing under shading stress. Plant Physiology and Biochemistry 130: 647-657 https://doi.org/10.1016/j.plaphy.2018.08.005
Markwell J, Osterman JC, Mitchell JL (1995) Calibration of the Minolta SPAD-502 leaf chlorophyll meter. Photosynthesis Research 46: 467–472
Monje OA, Bugbee B (1992) Inherent limitations of nondestructive chlorophyll meters: a comparison of two types of meters. Hortscience 27: 69–71
Moran JA, Mitchell AK, Goodmanson G, Stockburger KA (2000) Differentiation among effects of nitrogen fertilization treatments on conifer seedlings by foliar reflectance: a comparison of methods. Tree Physiology 20: 1113–1120
Peñuelas J, Filella I (1998) Visible and near-infrared reflectance techniques for diagnosing plant physiological status. Trends in Plant Science 3: 151–156
Percival GC, Keary IP, Noviss K (2008) The potential of a chlorophyll content SPAD meter to quantify nutrient stress in foliar tissue of sycamore (Acer pseudoplatanus), english oak (Quercus robur), and european beech (Fagus sylvatica). Arboriculture & Urban Forestry 34: 89–100
Pinkard EA, Patel V, Mohammed C (2006) Chlorophyll and nitrogen determination for plantation-grown Eucalyptus nitens and E. globules using a non-destructive meter. Forest Ecol 223: 211–217
Repo T, Launiainen S, Lehto T, Sutinen S, Ruhanen H, Heiskanen J, Laurén A, Silvennoinen R, Vapaavuori E, Finér L (2016) The responses of Scots pine seedlings to waterlogging during the growing season. Canadian Journal of Forest Research 46: 1439– 1450 https://doi.org/10.1139/cjfr-2015-0447
Richardson AD, Duigan SP, Berlyn GP (2002) An evaluation of noninvasive methods to estimate foliar chlorophyll content. New Phytologist 153: 185–194
Sardoel AS, Rahbarian P, Shahdadneghad M (2014) Evaluation chlorophyll contents assessment on three indoor ornomental plants with plant growth regulators. European Journal of Experimental Biology 4: 306-310